{"title":"Enzyme \u0026 Related Buffer for mRNA","description":"\u003cp\u003e\u003cstrong\u003eEssential Enzyme-Buffer Systems in mRNA Molecular Biology\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWithin the realm of mRNA research, specific enzymatic manipulations are often employed alongside tailored buffer systems to ensure efficient and reliable experimentation.\u003c\/p\u003e","products":[{"product_id":"poly-a-polymerase","title":"Poly(A) Polymerase","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n.bio-chart {\n    border: solid #3361ac 2px;\n    text-align: center;\n    margin-bottom: 15px;\n    border-radius: 12px;\n    box-shadow: 0px 0px 5px rgba(22, 47, 101, 0.5);\n}\n.bio-chart-title {\n    background-color: #162f65;\n    color: white;\n    display: flex;\n    padding: 5px 0;\n    border-radius: 9px 9px 0 0;\n}\n.bio-chart-row:nth-child(odd) {\n    background-color: #ebf6ff;\n    display: flex;\n    padding: 5px 0;\n}\n.bio-chart-row:nth-child(even) {\n    background-color: white;\n    display: flex;\n    padding: 5px 0;\n}\n.bio-chart-row:last-child {\n    border-radius: 0 0 12px 12px;\n}\n.chart-col-8 {\n    width: 66.67%;\n}\n.chart-col-4 {\n    width: 33.33%;\n}\n.chart-component {\n    color: #3361ac;\n    font-weight: bold;\n}\n.chart-parameter {\n    color: #162f65;\n}\n.operation-recommend-icon {\n    margin-top: 3px;\n}\n.operation-alert {\n    background-color: #ffecd9;\n    border-radius: 8px;\n    padding: 10px;\n    margin: 0 5px;\n    display: flex;\n    gap: 10px;\n    color: #c62828;\n    box-shadow: 0 0 5px rgba(22, 47, 101, 0.2);\n    margin-bottom: 20px;\n}\n.operation-alert-icon {\n    margin-top: 3px;\n}\n\u003c\/style\u003e\n\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003ePoly(A) Polymerase uses ATP as a substrate for template-independent addition of adenosine monophosphate to the 3'-hydroxyl termini of RNA molecules.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e: Recombinant \u003cem\u003eE.coli\u003c\/em\u003e\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 5U\/μL\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit is defined as the amount of enzyme that will incorporate 1nmol of AMP into RNA in a 20μL volume in 10 minutes at 37°C.\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 20mM Tris-HCl (pH7.5@25℃), 1mM EDTA, 300mM NaCl, 1mM DTT, 0.1%(v\/v) Triton X-100,50%(v\/v) glycerol.\u003cbr\u003e\u003cstrong\u003eCompanion Product\u003c\/strong\u003e: \u003ca href=\"https:\/\/shop.hongene.com\/products\/10x-poly-a-polymerase-reaction-buffer\"\u003e10X poly(A) Polymerase Reaction Buffer\u003c\/a\u003e, Cat#ON-127, 500mM Tris-HCl, (pH7.9@25℃), 2.5M NaCl, 100mM MgCl\u003csub\u003e2\u003c\/sub\u003e.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eProtocol\u003c\/strong\u003e: The standard reaction will produce ～150bp long poly(A)-tail on up to 10μg of capped or uncapped RNA from IVT reactions and co-transcriptional capping reactions need to be purified before adding to the poly(A)-tailing reaction.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eCombine the following reagents:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"bio-chart\"\u003e\n\u003cdiv class=\"bio-chart-title\"\u003e\n\u003cdiv class=\"chart-col-8\"\u003eComponent\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4\"\u003eAmount\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003eRNA\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e1-10μg\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003e10X poly(A) Polymerase Reaction Buffer\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e2μL\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003eATP (10mM)\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e2μL\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003ePoly(A) Polymerase\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e20 units\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003e(Optional) RNase Inhibitor\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e25μL\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003eNuclease-free H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003eto 20μL\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003col start=\"2\"\u003e\n\u003cli\u003eIncubate reaction at 37°C for 60 minutes.\u003c\/li\u003e\n\u003cli\u003eStop reaction by adding EDTA to final concentration of \u0026gt;11mM.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"operation-alert\"\u003e\n\u003cdiv class=\"operation-alert-icon\"\u003e\u003csvg viewbox=\"0 0 16 16\" fill=\"currentColor\" height=\"16\" width=\"16\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"\u003e\n      \u003cpath d=\"M8 15A7 7 0 1 1 8 1a7 7 0 0 1 0 14m0 1A8 8 0 1 0 8 0a8 8 0 0 0 0 16\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M7.002 11a1 1 0 1 1 2 0 1 1 0 0 1-2 0M7.1 4.995a.905.905 0 1 1 1.8 0l-.35 3.507a.552.552 0 0 1-1.1 0z\"\u003e\u003c\/path\u003e\n    \u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv\u003eBefore poly(A)-tail reaction, heat-denaturation of the RNA may improve the efficiency of adding poly(A)-tail.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e: -20℃\u003cbr\u003e\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e: Free of endonuclease, exonuclease and RNase activities.\u003cbr\u003e\u003cstrong\u003ePhysical Purity\u003c\/strong\u003e: ＞95% by SDS-PAGE.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003cp\u003e\u003ccite\u003e1. Bernstein P and Ross J (1989) Poly(A), poly(A) binding protein and the regulation of mRNA stability. Trends Biochem Sci 14: 373–377.\u003c\/cite\u003e\u003cbr\u003e\u003ccite\u003e2. Gallie DR (1991) The cap and poly(A) tail function synergistically to regulate mRNA translational efficiency. Genes Dev 5: 2108–2116.\u003c\/cite\u003e\u003cbr\u003e\u003ccite\u003e3. Krug, M.S. and Berger, S.L. (1987) Methods Enzymol. 152, 262.\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ 1kU \/ centrifuge tube","offer_id":44541425909960,"sku":"ON-126-KU001-P","price":225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_cf554ec8-96cb-43c6-bea1-b1550270cc8e.jpg?v=1748588757"},{"product_id":"alkaline-phosphatase-tab5","title":"Alkaline Phosphatase, TAB5","description":"\u003cstyle\u003e\n.bio-chart {\n    border: solid #3361ac 2px;\n    text-align: center;\n}\n.bio-chart-title {\n    background-color: #162f65;\n    color: white;\n    display: flex;\n    padding: 3px 0px;\n}\n.bio-chart-content-1 {\n    background-color: #ebf6ff;\n    display: flex;\n    align-items: center;\n    padding: 3px 0px;\n}\n.bio-chart-content-2 {\n    background-color: white;\n    display: flex;\n    align-items: center;\n    padding: 3px 0px;\n}\n.chart-col-8 {\n    width: 66.67%;\n}\n.chart-col-4 {\n    width: 33.33%;\n}\n.chart-component {\n    color: #3361ac;\n    font-weight: bold;\n}\n.chart-parameter {\n    color: #162f65;\n}\n.chart-col-6 {\n    width: 50%;\n}\n.chart-col-3 {\n    width: 25%;\n}\n\u003c\/style\u003e\n\n\u003ch3\u003eProduct Information\u003c\/h3\u003e\n \u003cp\u003e\u003cstrong\u003eProduct description\u003c\/strong\u003e: Alkaline Phosphatase, TAB5 nonspecifically catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. It also hydrolyses NTPs and dNTPs. It is completely inactivated by heating at 80℃ for 2 minutes.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSource\u003c\/strong\u003e: Recombinant E.coli\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 2.5U\/μL\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit is defined as the amount of enzyme that will dephosphorylate 1μg of pUC19 vector DNA cut with a restriction enzyme generating 5' recessed ends in 30 minutes at 37℃. Dephosphorylation is defined as ＞ 95% inhibition of recircularization in a self-ligation reaction and is measured by transformation into DH5α.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 10mM Tris-HCl , 1mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 0.01mM ZnCl\u003csub\u003e2\u003c\/sub\u003e, 50%(v\/v) glycerol, pH7.4, 25℃\u003cbr\u003e10X Phosphatase Reaction Buffer: 500mM Bis-Tris-Propane-HCl , 10mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 1mM ZnCl\u003csub\u003e2\u003c\/sub\u003e, pH6, 25℃\u003cbr\u003e10X IVT Reaction Buffer without Mg\u003csup\u003e2+\u003c\/sup\u003e, Cat#ON-41, 400mM Tris PH7.9, 100mM DTT, 20mM Spermidine\u003c\/p\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003cbr\u003e\n \u003cp\u003e\u003cstrong\u003eProtocol:\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\n\u003cstrong\u003eDNA as substance:\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eCombine the follows on ice:\u003c\/li\u003e\n\u003c\/ol\u003e\n  \u003cdiv class=\"bio-chart\"\u003e\n    \u003cdiv class=\"bio-chart-title\"\u003e\n      \u003cdiv class=\"chart-col-8\"\u003eComponent\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4\"\u003eAmount\u003c\/div\u003e\n    \u003c\/div\u003e\n    \u003cdiv class=\"bio-chart-content-1\"\u003e\n      \u003cdiv class=\"chart-col-8 chart-component\"\u003e5'-Phosphate-DNA\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4 chart-parameter\"\u003e1μg\u003c\/div\u003e\n    \u003c\/div\u003e\n    \u003cdiv class=\"bio-chart-content-2\"\u003e\n      \u003cdiv class=\"chart-col-8 chart-component\"\u003e10X Phosphatase Reaction Buffer\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4 chart-parameter\"\u003e2μL\u003c\/div\u003e\n    \u003c\/div\u003e\n    \u003cdiv class=\"bio-chart-content-1\"\u003e\n      \u003cdiv class=\"chart-col-8 chart-component\"\u003eAlkaline Phosphatase, TAB5\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4 chart-parameter\"\u003e2.5-5 units\u003c\/div\u003e\n    \u003c\/div\u003e\n    \u003cdiv class=\"bio-chart-content-2\"\u003e\n      \u003cdiv class=\"chart-col-8 chart-component\"\u003eNuclease-free H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4 chart-parameter\"\u003eto 20μL\u003c\/div\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\u003cbr\u003e\n\u003col start=\"2\"\u003e\n\u003cli\u003eIncubate at 37℃, 30min\u003c\/li\u003e\n\u003cli\u003e(optional) Heat inactivity enzyme by incubating at 80℃, 2min.\u003c\/li\u003e\n\u003c\/ol\u003e\n \u003cp\u003e\u003cstrong\u003eRNA as substance:\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eHeat Denature the RNA:\u003c\/li\u003e\n\u003c\/ol\u003e\n  \u003cdiv class=\"bio-chart\"\u003e\n    \u003cdiv class=\"bio-chart-title\"\u003e\n      \u003cdiv class=\"chart-col-8\"\u003eComponent\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4\"\u003eAmount\u003c\/div\u003e\n    \u003c\/div\u003e\n    \u003cdiv class=\"bio-chart-content-1\"\u003e\n      \u003cdiv class=\"chart-col-8 chart-component\"\u003e5'-Phosphate-RNA\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4 chart-parameter\"\u003e1μg\u003c\/div\u003e\n    \u003c\/div\u003e\n    \u003cdiv class=\"bio-chart-content-2\"\u003e\n      \u003cdiv class=\"chart-col-8 chart-component\"\u003eNuclease-free H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4 chart-parameter\"\u003eto 15μL\u003c\/div\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\u003cbr\u003e\n\u003col start=\"2\"\u003e\n\u003cli\u003eIncubate at 65℃, 10~15min, transfer the tube immediately to ice.\u003c\/li\u003e\n\u003cli\u003eOn ice, combine the following reaction components in the order given:\u003c\/li\u003e\n\u003c\/ol\u003e\n  \u003cdiv class=\"bio-chart\"\u003e\n    \u003cdiv class=\"bio-chart-title\"\u003e\n      \u003cdiv class=\"chart-col-8\"\u003eComponent\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4\"\u003eAmount\u003c\/div\u003e\n    \u003c\/div\u003e\n    \u003cdiv class=\"bio-chart-content-1\"\u003e\n      \u003cdiv class=\"chart-col-8 chart-component\"\u003eHeat denatured RNA from step 1 (above)\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4 chart-parameter\"\u003e15μL\u003c\/div\u003e\n    \u003c\/div\u003e\n    \u003cdiv class=\"bio-chart-content-2\"\u003e\n      \u003cdiv class=\"chart-col-8 chart-component\"\u003e10X Phosphatase Reaction Buffer\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4 chart-parameter\"\u003e2μL\u003c\/div\u003e\n    \u003c\/div\u003e\n    \u003cdiv class=\"bio-chart-content-1\"\u003e\n      \u003cdiv class=\"chart-col-8 chart-component\"\u003eAlkaline Phosphatase, TAB5\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4 chart-parameter\"\u003e2.5-5 units\u003c\/div\u003e\n    \u003c\/div\u003e\n    \u003cdiv class=\"bio-chart-content-2\"\u003e\n      \u003cdiv class=\"chart-col-8 chart-component\"\u003eRNase Inhibitor (Recommend)\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4 chart-parameter\"\u003e20 units\u003c\/div\u003e\n    \u003c\/div\u003e\n    \u003cdiv class=\"bio-chart-content-1\"\u003e\n      \u003cdiv class=\"chart-col-8 chart-component\"\u003eTotal Reaction Volume\u003c\/div\u003e\n      \u003cdiv class=\"chart-col-4 chart-parameter\"\u003e20μL\u003c\/div\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\u003cbr\u003e\n\u003col start=\"4\"\u003e\n\u003cli\u003eIncubate at 37℃, 30min.\u003c\/li\u003e\n\u003cli\u003eStop the reaction using any one of the following methods:\u003cbr\u003ea) immediate storage at –20℃ or –70℃.\u003cbr\u003eb) Add of EDTA to a final concentration of \u0026gt; 2mM.\u003cbr\u003ec) Phenol\/chloroform extract then precipitate using salt\/alcohol.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n \u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e: -20℃\u003cbr\u003e\u003cbr\u003e\n\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e: Free of endonuclease, exonuclease, nickase and RNase activities.\u003cbr\u003e\u003cbr\u003e\n\u003cstrong\u003ePhysical Purity\u003c\/strong\u003e: ＞90% by SDS-PAGE.\u003c\/p\u003e\n\u003chr style=\"background: linear-gradient(to right, white , #162F65); border: 0; height: 1px;\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n \u003cp\u003e\u003ccite\u003e1. Maria Rina, Charalambos Pozidis, et al. Eur. J. Biochem. 267, 1230-1238(2000).\u003c\/cite\u003e\u003c\/p\u003e\n \u003cp\u003e\u003ccite\u003e2. Ellen Wang, Dimitris Koutsioulis, et al. J. Mol. Biol. 366, 1318-1331(2007).\u003c\/cite\u003e\u003c\/p\u003e\n \u003cp\u003e\u003ccite\u003e3. Ping Li, Jon Beckwith, et al. Proc. Natl. Acad. Sci. USA.85,7685-7689(1988).\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ 1kU \/ centrifuge tube","offer_id":44541426073800,"sku":"ON-179-KU001-P","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_338d28a6-e687-4284-8616-e4da8333e31f.jpg?v=1748588757"},{"product_id":"rnase-r","title":"RNase R","description":"\u003cstyle\u003e\n    .rte-operation-img {\n    padding: 10px;\n    margin: 5px 5px 25px;\n    border-radius: 12px;\n    box-shadow: 0 0 5px rgba(22, 47, 101, 0.3);\n    display: flex; \n    justify-content: center;\n    overflow: hidden;\n}\n    .rte-operation-img img {\n        width: auto;\n        max-height: 500px;\n    }\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n.operation-alert {\n    background-color: #ffecd9;\n    border-radius: 8px;\n    padding: 10px;\n    margin: 0 5px;\n    display: flex;\n    gap: 10px;\n    color: #c62828;\n    box-shadow: 0 0 5px rgba(22, 47, 101, 0.3);\n    margin-bottom: 20px;\n}\n.operation-alert-icon {\n    margin-top: 3px;\n}\n\u003c\/style\u003e\n\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003eRibonuclease R (RNase R) from E. coli, is a magnesium-dependent 3' to 5' exoribonuclease that digests essentially all linear RNAs but does not digest lariat or circular RNA structures, or double-stranded RNA with 3'-overhangs shorter than 7 nucleotides. RNase R digests RNA from the 3' to 5' direction, releasing 5'-monophosphate nucleotides to produce dinucleotide and trinucleotide end products.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResource\u003c\/strong\u003e: Recombinant \u003cem\u003eE.coli\u003c\/em\u003e\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 20U\/μL\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit converts 1μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37℃.\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 50mM Tris-HCl, 0.1mM EDTA, 100mM NaCl, 1mM DTT, 0.1%(v\/v)Triton X-100, 50%(v\/v) glycerol, pH7.5@25℃.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003cdiv class=\"rte-operation-img\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Cyclic_RNA_enzymatic_synthesis-EN.jpg.png?v=1741326056\"\u003e\u003c\/div\u003e\n\u003cp\u003eT4 RNA ligase 1, T4 RNA ligase 2, RNaseR on enzymatic synthesis of circular RNA:\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIVT reaction to obtain linear RNA\u003c\/strong\u003e: it is worth noting that for this step of IVT reaction, a certain proportion of GMP needs to be doped to obtain GMP-containing linear RNA;\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRNA cyclisation\u003c\/strong\u003e: circular RNA is produced by joining the two ends within the RNA molecule by T4 RNA ligase 1 (T4 Rnl1) or T4 RNA ligase 2 (T4 Rnl2) catalysing the joining of 5'-phosphate and 3'-hydroxyl groups at the RNA terminus;\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRemoval of linear RNA\u003c\/strong\u003e: RNaseR is able to remove all linear RNA molecules from the reaction solution, resulting in linear RNA-free circular RNA.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e: Stored at -20±5°C.\u003c\/p\u003e\n\u003cdiv class=\"operation-alert\"\u003e\n\u003cdiv class=\"operation-alert-icon\"\u003e\u003csvg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"16\" height=\"16\" fill=\"currentColor\" viewbox=\"0 0 16 16\"\u003e\n      \u003cpath d=\"M8 15A7 7 0 1 1 8 1a7 7 0 0 1 0 14m0 1A8 8 0 1 0 8 0a8 8 0 0 0 0 16\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M7.002 11a1 1 0 1 1 2 0 1 1 0 0 1-2 0M7.1 4.995a.905.905 0 1 1 1.8 0l-.35 3.507a.552.552 0 0 1-1.1 0z\"\u003e\u003c\/path\u003e\n    \u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eDo not store in a frost-free freezer.\u003c\/strong\u003e Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eFunctional Assay: Passes the internal function testing.\u003c\/li\u003e\n\u003cli\u003eContaminant Assay: Free of detectable Endonuclease and Exonuclease.\u003c\/li\u003e\n\u003cli\u003eEndotoxin Assay: Pass.\u003c\/li\u003e\n\u003cli\u003ePhysical Purity: \u0026gt;95% by SDS-PAGE with Coomassie® blue staining.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ 20kU \/ centrifuge tube","offer_id":44541426565320,"sku":"ON-509-C020-KU020-P","price":3400.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_8bc65099-6d35-4b9b-8ec1-c1a9f52d435c.jpg?v=1748588756"},{"product_id":"mrna-cap-2-o-methyltransferase","title":"mRNA Cap-2'-O-Methyltransferase","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n.rte-companion-products {\n    display: flex;\n    gap: 10px;\n    margin-bottom: 25px;\n}\n.rte-companion-link {\n    padding: 8px;\n    margin: 5px;\n    width: 50%;\n    max-width: 450px;\n    border-radius: 8px;\n    box-shadow: 0 0 5px #162f6580;\n    text-decoration: none !important;\n    transition: transform 0.3s;\n}\n.rte-companion-link:hover {\n    transform: translateY(-5px);\n}\n.rte-companion-product {\n    display: flex;\n    gap: 10px;\n}\n.companion-product-img {\n    width: 46%;\n    min-width: 150px;\n}\n.companion-product-content {\n    width: 50%;\n}\n.rte-product-catalog, .rte-product-volumn {\n    font-size: 14px;\n    display: block;\n    margin-top: 5px;\n    color: #162f65;\n}\n.rte-product-title {\n    color: #3361ac;\n    font-weight: 700;\n    display: block;\n    margin-bottom: 5px;\n}\n@media (max-width: 768px) {\n.rte-companion-products {\n    flex-direction: column;\n}\n.rte-companion-link {\n    width: 95%;\n}\n}\n\u003c\/style\u003e\n\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003emRNA Cap-2'-O-Methyltransferase is a recombinant protein from Vaccinia virus. The enzyme can add a methyl group at the 2'-O position of the first nucleotide adjacent to the Cap structure at the 5' end of the RNA. The enzyme utilizes SAM as a methyl donor to methylate capped RNA (Cap 0) resulting in a cap-1 structure. The Cap-1 structure has been reported to enhance mRNA translation efficiency and hence may help improve expression in mRNA transfection and microinjection experiments. mRNA cap-2'-O-Methyltransferase specifically requires RNA with an m7GpppN Cap as substrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e: Recombinant \u003cem\u003eE.coli\u003c\/em\u003e\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 50U\/μL\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit is defined as the amount of enzyme required to methylate 10pmols of 80 nt Capped RNA transcript in 1 hour at 37℃.\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 20mM Tris-HCl (pH8.0), 0.1mM EDTA, 100mM NaCl, 1mM DTT, 0.1%(v\/v) Triton X-100, 50%(v\/v) glycerol.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompanion Products:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"rte-companion-products\"\u003e\n\u003ca class=\"rte-companion-link\" href=\"https:\/\/shop.hongene.com\/products\/10x-capping-buffer\" title=\"jump to ON-073\"\u003e\n\u003cdiv class=\"rte-companion-product\"\u003e\n\u003cdiv class=\"companion-product-img\"\u003e\u003csvg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" x=\"0px\" y=\"0px\" viewbox=\"0 0 600 600\" style=\"enable-background: new 0 0 600 600; 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margin-bottom: 5px;\"\u003einside with:\u003c\/div\u003e\n\u003cdiv\u003e- 500mM Tris-HCl, pH8.0\u003cbr\u003e- 50mM KCl\u003cbr\u003e- 10mM MgCl\u003csub\u003e2\u003c\/sub\u003e\u003cbr\u003e- 10mM DTT;\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\u003ca class=\"rte-companion-link\" href=\"https:\/\/shop.hongene.com\/products\/s-adenosylmethionine-sam\" title=\"jump to ON-074\"\u003e\n\u003cdiv class=\"rte-companion-product\"\u003e\n\u003cdiv class=\"companion-product-img\"\u003e\u003csvg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" x=\"0px\" y=\"0px\" viewbox=\"0 0 600 600\" style=\"enable-background: new 0 0 600 600; border-radius: 8px; border: solid 1px #162f65;\" xml:space=\"preserve\"\u003e\n        \u003cstyle type=\"text\/css\"\u003e\n\t.st0{fill:#BFBFBF;}\n\t.st1{fill:none;stroke:#bfbfbf;stroke-width:21.1099;stroke-miterlimit:10;}\n\t.st2{fill:none;stroke:#bfbfbf;stroke-width:24.3757;stroke-miterlimit:10;}\n\u003c\/style\u003e\n        \u003cg\u003e\n          \u003cpath class=\"st0\" d=\"M282.4,390.4c-9.79,0-17.75,7.96-17.75,17.75c0,9.79,7.96,17.76,17.75,17.76c9.8,0,17.76-7.97,17.76-17.76\n\t\tC300.17,398.37,292.21,390.4,282.4,390.4z M282.4,418.81c-3.8,0-7.32-2.03-9.22-5.33c-1.9-3.3-1.9-7.36,0-10.65\n\t\tc1.91-3.3,5.43-5.33,9.23-5.33c5.89,0.01,10.65,4.78,10.65,10.66C293.07,414.05,288.3,418.81,282.4,418.81z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st0\" d=\"M300,446.01c-6.59,0-11.94,5.36-11.94,11.94c0,6.59,5.36,11.95,11.94,11.95c6.59,0,11.95-5.36,11.95-11.95\n\t\tC311.94,451.37,306.59,446.01,300,446.01z M300,465.12c-2.56,0-4.92-1.37-6.2-3.59c-1.28-2.22-1.28-4.95,0-7.17\n\t\tc1.29-2.22,3.65-3.59,6.21-3.59c3.96,0.01,7.17,3.22,7.17,7.17C307.17,461.91,303.96,465.12,300,465.12z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st0\" d=\"M311.34,314.76c-14.11,0-25.58,11.47-25.58,25.58c0,14.11,11.47,25.6,25.58,25.6c14.12,0,25.6-11.49,25.6-25.6\n\t\tC336.94,326.23,325.47,314.76,311.34,314.76z M311.34,355.7c-5.48,0-10.55-2.93-13.29-7.68c-2.74-4.75-2.74-10.6,0-15.35\n\t\tc2.75-4.75,7.82-7.68,13.3-7.68c8.49,0.01,15.35,6.89,15.35,15.37C326.71,348.83,319.83,355.7,311.34,355.7z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st1\" d=\"M357.17,132.88v312.25c0,0.3,0,0.58-0.02,0.88c-0.46,31.17-25.88,56.29-57.16,56.29s-56.7-25.12-57.16-56.29\n\t\tc-0.02-0.3-0.02-0.58-0.02-0.88V132.88H357.17z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st0\" d=\"M299.99,445.13c0,0.3,0,0.58,0.02,0.88h-0.02V445.13z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st0\" d=\"M300.01,445.13v0.88h-0.02C300.01,445.71,300.01,445.43,300.01,445.13z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st2\" d=\"M366.41,132.88H233.59c-9.68,0-17.59-7.92-17.59-17.59v0c0-9.68,7.92-17.59,17.59-17.59h132.82\n\t\tc9.68,0,17.59,7.92,17.59,17.59v0C384,124.96,376.08,132.88,366.41,132.88z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st1\" d=\"M242.84,257.19c0,0,79.13,35.97,114.33-40.66\"\u003e\u003c\/path\u003e\n        \u003c\/g\u003e\n      \u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv class=\"companion-product-content\"\u003e\n\u003cdiv class=\"rte-product-catalog\"\u003eCat#ON-074\u003c\/div\u003e\n\u003cdiv class=\"rte-product-title\"\u003eS-adenosylmethionine (SAM),32mM\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\n\u003c\/div\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan style=\"background-color: #ffecd9; border-radius: 4px; color: #c62828; padding: 0 5px;\"\u003e\u003csvg style=\"margin: 0 5px -2px 0;\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"16\" height=\"16\" fill=\"currentColor\" viewbox=\"0 0 16 16\"\u003e\n      \u003cpath d=\"M8 15A7 7 0 1 1 8 1a7 7 0 0 1 0 14m0 1A8 8 0 1 0 8 0a8 8 0 0 0 0 16\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M7.002 11a1 1 0 1 1 2 0 1 1 0 0 1-2 0M7.1 4.995a.905.905 0 1 1 1.8 0l-.35 3.507a.552.552 0 0 1-1.1 0z\"\u003e\u003c\/path\u003e\n      \u003c\/svg\u003eDo not resuspend the RNA in an EDTA-containing solution.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eTo enhance the stability of RNA in solution, RNase inhibitor is desired, which can be add to a final concertation of 1U\/ul at the time of reaction set-up.\u003c\/li\u003e\n\u003cli\u003eSAM is unstable at pH 7-8, 37℃ and should be mixed fresh for each reaction series. We recommend determining how many reactions will be performed and diluting an aliquot of the 32mM stock to 2mM.\u003c\/li\u003e\n\u003cli\u003eHeating the solution of RNA prior to incubation with Vaccinia capping enzyme, to remove secondary structure on the 5' end of the transcripts. For highly structured 5' ends, increase the RNA heat-denaturation conditions used. For example, 65℃ for 20 min, 75℃ for 10 min, 85℃ for 5 min, etc…\u003c\/li\u003e\n\u003cli\u003eVaccinia Capping Enzyme and 2'-O-methyltransferase work together to produce the Cap 1 structure.\u003c\/li\u003e\n\u003cli\u003eCapping reaction incubation time can be Increased up to 3 hours at 37℃ for a high capping efficiency for transcripts with known structured 5' ends.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e: -20℃\u003cbr\u003e\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e: Free of Endonuclease, Exonuclease, and RNase activities.\u003cbr\u003e\u003cstrong\u003ePhysical Purity\u003c\/strong\u003e: ＞95% by SDS-PAGE.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003cp\u003e\u003ccite\u003e1. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 250, No. 24, Issue of December 25, pp. 9322-9329,1975.\u003c\/cite\u003e\u003cbr\u003e\u003ccite\u003e2. Kuge, H. et al., (1998) Nucl. Acids Res. 26, 3208.\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ Non-GMP \/ 5kU \/ centrifuge tube","offer_id":44541427417288,"sku":"ON-014-KU005-P","price":75.0,"currency_code":"USD","in_stock":true},{"title":"M (Molecular Biology) \/ GMP \/ 5kU \/ centrifuge tube","offer_id":44755972817096,"sku":"ON-014-G","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_a5395b90-056d-4efa-9873-f83ddc9d0265.jpg?v=1748588754"},{"product_id":"rnase-iii-e-coli","title":"RNase III, E.coli","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n    .rte-companion-products {\n    display: flex;\n    flex-wrap: wrap;\n    gap: 10px;\n    margin-bottom: 25px;\n}\n.rte-companion-link {\n    padding: 8px;\n    margin: 5px;\n    width: 45%;\n    border-radius: 8px;\n    box-shadow: 0 0 5px #162f6580;\n    text-decoration: none !important;\n    transition: transform 0.3s;\n}\n.rte-companion-link:hover {\n    transform: translateY(-5px);\n}\n.rte-companion-product {\n    display: flex;\n    gap: 10px;\n}\n.companion-product-img {\n    width: 46%;\n    min-width: 150px;\n}\n.companion-product-content {\n    width: 50%;\n}\n.rte-product-catalog, .rte-product-volumn {\n    font-size: 14px;\n    display: block;\n    margin-top: 5px;\n    color: #162f65;\n}\n.rte-product-title {\n    color: #3361ac;\n    font-weight: 700;\n    display: block;\n    margin-bottom: 5px;\n}\n@media (max-width: 768px) {\n.rte-companion-products {\n    flex-direction: column;\n}\n.rte-companion-link {\n    width: 75%;\n}\n}\n    @media (max-width: 576px) {\n.rte-companion-link {\n    width: 95%;\n}\n}\n.bio-chart {\n    border: solid #3361ac 2px;\n    text-align: center;\n    margin-bottom: 15px;\n    border-radius: 12px;\n    box-shadow: 0px 0px 5px rgba(22, 47, 101, 0.5);\n}\n.bio-chart-title {\n    background-color: #162f65;\n    color: white;\n    display: flex;\n    padding: 5px 0;\n    border-radius: 9px 9px 0 0;\n}\n.bio-chart-row:nth-child(odd) {\n    background-color: #ebf6ff;\n    display: flex;\n    padding: 5px 0;\n}\n.bio-chart-row:nth-child(even) {\n    background-color: white;\n    display: flex;\n    padding: 5px 0;\n}\n.bio-chart-row:last-child {\n    border-radius: 0 0 12px 12px;\n}\n.chart-col-8 {\n    width: 66.67%;\n}\n.chart-col-4 {\n    width: 33.33%;\n}\n.chart-component {\n    color: #3361ac;\n    font-weight: bold;\n}\n.chart-parameter {\n    color: #162f65;\n}\n    .operation-recommend {\n    background-color: #c1d4e7;\n    border-radius: 8px;\n    padding: 10px;\n    margin: 0 5px;\n    display: flex;\n    gap: 10px;\n    color: #162f65;\n    box-shadow: 0 0 5px rgba(22, 47, 101, 0.2);\n    margin-bottom: 12px;\n}\n.operation-recommend-icon {\n    margin-top: 3px;\n}\n    .operation-alert {\n    background-color: #ffecd9;\n    border-radius: 8px;\n    padding: 10px;\n    margin: 0 5px;\n    display: flex;\n    gap: 10px;\n    color: #c62828;\n    box-shadow: 0 0 5px rgba(22, 47, 101, 0.2);\n    margin-bottom: 20px;\n}\n.operation-alert-icon {\n    margin-top: 3px;\n}\n\u003c\/style\u003e\n\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003eRNase III, E.coli is a homodimeric, divalent metal ion dependent nuclease. RNase III cleaves long double-stranded RNA (dsRNA) into short 12-30 base dsRNAs containing a 5'-PO\u003csub\u003e4\u003c\/sub\u003e, 3'-OH, and a dinucleotide 3' overhang.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e: Recombinant \u003cem\u003eE.coli\u003c\/em\u003e\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 0.5U\/μL\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit is the amount of enzyme required to digest 1μg of dsRNA to siRNA in 20 minutes at 37℃ in a total reaction volume of 50μL.\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 500mM NaCl, 20mM Tris-HCl (pH 8.0, 25℃), 0.5mM EDTA, 0.5mM DTT, 50% Glyercol.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompanion Buffer:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"rte-companion-products\"\u003e\n\u003ca title=\"jump to ON-078\" href=\"https:\/\/shop.hongene.com\/products\/10x-rnase-iii-reaction-buffer\" class=\"rte-companion-link\"\u003e\n\u003cdiv class=\"rte-companion-product\"\u003e\n\u003cdiv class=\"companion-product-img\"\u003e\u003csvg xml:space=\"preserve\" style=\"enable-background: new 0 0 600 600; 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margin-bottom: 5px;\"\u003einside with:\u003c\/div\u003e\n\u003cdiv\u003e- 500mM Tris-HCl (pH7.5, 25℃)\u003cbr\u003e- 500mM NaCl\u003cbr\u003e- 10mM DTT\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\u003ca title=\"jump to ON-079\" href=\"https:\/\/shop.hongene.com\/products\/10x-mncl2\" class=\"rte-companion-link\"\u003e\n\u003cdiv class=\"rte-companion-product\"\u003e\n\u003cdiv class=\"companion-product-img\"\u003e\u003csvg xml:space=\"preserve\" style=\"enable-background: new 0 0 600 600; border-radius: 8px; border: solid 1px #162f65;\" viewbox=\"0 0 600 600\" y=\"0px\" x=\"0px\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" version=\"1.1\"\u003e\n        \u003cstyle type=\"text\/css\"\u003e\n\t.st0{fill:#BFBFBF;}\n\t.st1{fill:none;stroke:#bfbfbf;stroke-width:21.1099;stroke-miterlimit:10;}\n\t.st2{fill:none;stroke:#bfbfbf;stroke-width:24.3757;stroke-miterlimit:10;}\n\u003c\/style\u003e\n        \u003cg\u003e\n          \u003cpath d=\"M282.4,390.4c-9.79,0-17.75,7.96-17.75,17.75c0,9.79,7.96,17.76,17.75,17.76c9.8,0,17.76-7.97,17.76-17.76\n\t\tC300.17,398.37,292.21,390.4,282.4,390.4z M282.4,418.81c-3.8,0-7.32-2.03-9.22-5.33c-1.9-3.3-1.9-7.36,0-10.65\n\t\tc1.91-3.3,5.43-5.33,9.23-5.33c5.89,0.01,10.65,4.78,10.65,10.66C293.07,414.05,288.3,418.81,282.4,418.81z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M300,446.01c-6.59,0-11.94,5.36-11.94,11.94c0,6.59,5.36,11.95,11.94,11.95c6.59,0,11.95-5.36,11.95-11.95\n\t\tC311.94,451.37,306.59,446.01,300,446.01z M300,465.12c-2.56,0-4.92-1.37-6.2-3.59c-1.28-2.22-1.28-4.95,0-7.17\n\t\tc1.29-2.22,3.65-3.59,6.21-3.59c3.96,0.01,7.17,3.22,7.17,7.17C307.17,461.91,303.96,465.12,300,465.12z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M311.34,314.76c-14.11,0-25.58,11.47-25.58,25.58c0,14.11,11.47,25.6,25.58,25.6c14.12,0,25.6-11.49,25.6-25.6\n\t\tC336.94,326.23,325.47,314.76,311.34,314.76z M311.34,355.7c-5.48,0-10.55-2.93-13.29-7.68c-2.74-4.75-2.74-10.6,0-15.35\n\t\tc2.75-4.75,7.82-7.68,13.3-7.68c8.49,0.01,15.35,6.89,15.35,15.37C326.71,348.83,319.83,355.7,311.34,355.7z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M357.17,132.88v312.25c0,0.3,0,0.58-0.02,0.88c-0.46,31.17-25.88,56.29-57.16,56.29s-56.7-25.12-57.16-56.29\n\t\tc-0.02-0.3-0.02-0.58-0.02-0.88V132.88H357.17z\" class=\"st1\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M299.99,445.13c0,0.3,0,0.58,0.02,0.88h-0.02V445.13z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M300.01,445.13v0.88h-0.02C300.01,445.71,300.01,445.43,300.01,445.13z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M366.41,132.88H233.59c-9.68,0-17.59-7.92-17.59-17.59v0c0-9.68,7.92-17.59,17.59-17.59h132.82\n\t\tc9.68,0,17.59,7.92,17.59,17.59v0C384,124.96,376.08,132.88,366.41,132.88z\" class=\"st2\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M242.84,257.19c0,0,79.13,35.97,114.33-40.66\" class=\"st1\"\u003e\u003c\/path\u003e\n        \u003c\/g\u003e\n      \u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv class=\"companion-product-content\"\u003e\n\u003cdiv class=\"rte-product-catalog\"\u003eCat#ON-079\u003c\/div\u003e\n\u003cdiv class=\"rte-product-title\"\u003e10X MnCl\u003csub\u003e2\u003c\/sub\u003e, ,\u003c\/div\u003e\n\u003cdiv class=\"rte-product-volumn\"\u003e\n\u003cdiv style=\"font-size: 12px; margin-bottom: 5px;\"\u003einside with:\u003c\/div\u003e\n\u003cdiv\u003e- 200mM MnCl\u003csub\u003e2\u003c\/sub\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\u003ca title=\"jump to ON-080\" href=\"https:\/\/shop.hongene.com\/products\/10x-edta\" class=\"rte-companion-link\"\u003e\n\u003cdiv class=\"rte-companion-product\"\u003e\n\u003cdiv class=\"companion-product-img\"\u003e\u003csvg xml:space=\"preserve\" style=\"enable-background: new 0 0 600 600; border-radius: 8px; border: solid 1px #162f65;\" viewbox=\"0 0 600 600\" y=\"0px\" x=\"0px\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" version=\"1.1\"\u003e\n        \u003cstyle type=\"text\/css\"\u003e\n\t.st0{fill:#BFBFBF;}\n\t.st1{fill:none;stroke:#bfbfbf;stroke-width:21.1099;stroke-miterlimit:10;}\n\t.st2{fill:none;stroke:#bfbfbf;stroke-width:24.3757;stroke-miterlimit:10;}\n\u003c\/style\u003e\n        \u003cg\u003e\n          \u003cpath d=\"M282.4,390.4c-9.79,0-17.75,7.96-17.75,17.75c0,9.79,7.96,17.76,17.75,17.76c9.8,0,17.76-7.97,17.76-17.76\n\t\tC300.17,398.37,292.21,390.4,282.4,390.4z M282.4,418.81c-3.8,0-7.32-2.03-9.22-5.33c-1.9-3.3-1.9-7.36,0-10.65\n\t\tc1.91-3.3,5.43-5.33,9.23-5.33c5.89,0.01,10.65,4.78,10.65,10.66C293.07,414.05,288.3,418.81,282.4,418.81z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M300,446.01c-6.59,0-11.94,5.36-11.94,11.94c0,6.59,5.36,11.95,11.94,11.95c6.59,0,11.95-5.36,11.95-11.95\n\t\tC311.94,451.37,306.59,446.01,300,446.01z M300,465.12c-2.56,0-4.92-1.37-6.2-3.59c-1.28-2.22-1.28-4.95,0-7.17\n\t\tc1.29-2.22,3.65-3.59,6.21-3.59c3.96,0.01,7.17,3.22,7.17,7.17C307.17,461.91,303.96,465.12,300,465.12z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M311.34,314.76c-14.11,0-25.58,11.47-25.58,25.58c0,14.11,11.47,25.6,25.58,25.6c14.12,0,25.6-11.49,25.6-25.6\n\t\tC336.94,326.23,325.47,314.76,311.34,314.76z M311.34,355.7c-5.48,0-10.55-2.93-13.29-7.68c-2.74-4.75-2.74-10.6,0-15.35\n\t\tc2.75-4.75,7.82-7.68,13.3-7.68c8.49,0.01,15.35,6.89,15.35,15.37C326.71,348.83,319.83,355.7,311.34,355.7z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M357.17,132.88v312.25c0,0.3,0,0.58-0.02,0.88c-0.46,31.17-25.88,56.29-57.16,56.29s-56.7-25.12-57.16-56.29\n\t\tc-0.02-0.3-0.02-0.58-0.02-0.88V132.88H357.17z\" class=\"st1\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M299.99,445.13c0,0.3,0,0.58,0.02,0.88h-0.02V445.13z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M300.01,445.13v0.88h-0.02C300.01,445.71,300.01,445.43,300.01,445.13z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M366.41,132.88H233.59c-9.68,0-17.59-7.92-17.59-17.59v0c0-9.68,7.92-17.59,17.59-17.59h132.82\n\t\tc9.68,0,17.59,7.92,17.59,17.59v0C384,124.96,376.08,132.88,366.41,132.88z\" class=\"st2\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M242.84,257.19c0,0,79.13,35.97,114.33-40.66\" class=\"st1\"\u003e\u003c\/path\u003e\n        \u003c\/g\u003e\n      \u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv class=\"companion-product-content\"\u003e\n\u003cdiv class=\"rte-product-catalog\"\u003eCat#ON-080\u003c\/div\u003e\n\u003cdiv class=\"rte-product-title\"\u003e10X EDTA\u003c\/div\u003e\n\u003cdiv class=\"rte-product-volumn\"\u003e\n\u003cdiv style=\"font-size: 12px; margin-bottom: 5px;\"\u003einside with:\u003c\/div\u003e\n\u003cdiv\u003e- 500mM EDTA\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\n\u003c\/div\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eRecommended protocol:\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eIn ice, combine the following, in order:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"bio-chart\"\u003e\n\u003cdiv class=\"bio-chart-title\"\u003e\n\u003cdiv class=\"chart-col-8\"\u003eComponent\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4\"\u003eAmount\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003eddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003eX μL\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003e10X RNase III Reaction Buffer\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e10μL\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003edsRNA\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003eX μL (10μg)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003eRNase III\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e10-20 units\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003e10X MnCl\u003csub\u003e2\u003c\/sub\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e10μL\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003eTotal Volume\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e100μL\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003col start=\"2\"\u003e\n\u003cli\u003eMix and incubate for 20 minutes at 37℃.\u003c\/li\u003e\n\u003cli\u003eAdd 10μL 10X EDTA to stop the reaction.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e:\u003cbr\u003e\u003cspan style=\"padding-left: 5px;\"\u003eLong term (Infrequent use): -70℃\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"padding-left: 5px;\"\u003eDaily\/Weekly use: -20℃*\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"padding-left: 5px;\"\u003eDo not store in a frost-free freezer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"operation-recommend\"\u003e\n\u003cdiv class=\"operation-recommend-icon\"\u003e\u003csvg viewbox=\"0 0 16 16\" class=\"bi bi-check-circle\" fill=\"currentColor\" height=\"16\" width=\"16\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"\u003e\n  \u003cpath d=\"M8 15A7 7 0 1 1 8 1a7 7 0 0 1 0 14m0 1A8 8 0 1 0 8 0a8 8 0 0 0 0 16\"\u003e\u003c\/path\u003e\n  \u003cpath d=\"m10.97 4.97-.02.022-3.473 4.425-2.093-2.094a.75.75 0 0 0-1.06 1.06L6.97 11.03a.75.75 0 0 0 1.079-.02l3.992-4.99a.75.75 0 0 0-1.071-1.05\"\u003e\u003c\/path\u003e\n\u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv\u003eAlways avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"operation-alert\"\u003e\n\u003cdiv class=\"operation-alert-icon\"\u003e\u003csvg viewbox=\"0 0 16 16\" fill=\"currentColor\" height=\"16\" width=\"16\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"\u003e\n      \u003cpath d=\"M8 15A7 7 0 1 1 8 1a7 7 0 0 1 0 14m0 1A8 8 0 1 0 8 0a8 8 0 0 0 0 16\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M7.002 11a1 1 0 1 1 2 0 1 1 0 0 1-2 0M7.1 4.995a.905.905 0 1 1 1.8 0l-.35 3.507a.552.552 0 0 1-1.1 0z\"\u003e\u003c\/path\u003e\n    \u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv\u003eRNase III stores at -20℃ may result in the formation of haze. If this happens, keep the tube at room temperature until tube temperature is up to 4~10℃. Then mix gently to clarify the solution. Haze should disappear. And there is negligible loss of activity.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e: Free of Endonuclease, Exonuclease, and Nickase activities. Pass functional testing\u003cbr\u003e\u003cstrong\u003ePhysical Purity\u003c\/strong\u003e: ＞90% by SDS-PAGE.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003cp\u003e\u003ccite\u003e1. Hugh D. Robertson, Robent E. Webster, and Norton D. Zinder. The Journal of Biological Chemistry. 243, 82-91(1968).\u003c\/cite\u003e\u003cbr\u003e\u003ccite\u003e2. Neelam Srivastava and Rai Ajit K Srivastava. Biochemistry and Molecular Biology International. 39, 171-180(1996).\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ 0.5kU \/ centrifuge tube","offer_id":44541427646664,"sku":"ON-024-U500-P","price":210.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_ca2c676a-bf00-424e-962d-5492c2c900a2.jpg?v=1748588751"},{"product_id":"eari","title":"EarI","description":"","brand":"Hongene","offers":[{"title":"M (Molecular Biology)","offer_id":44541440262344,"sku":"ON-430","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_feee6329-d1a8-4c8b-8d49-250fc1c790fb.jpg?v=1748588722"},{"product_id":"t7-enzyme-mix","title":"T7 Enzyme mix","description":"","brand":"Hongene","offers":[{"title":"M (Molecular Biology)","offer_id":44541441081544,"sku":"ON-211","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_2a94c6c9-a0d3-49ee-9928-beb81baf2d2a.jpg?v=1748588721"},{"product_id":"vaccinia-capping-enzyme","title":"Vaccinia Capping Enzyme","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n\u003c\/style\u003e\n\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003eVaccinia Capping enzyme is a recombinant protein from Vaccinia virus. Vaccinia Capping Enzyme can add 7-methylguanylate cap structure (Cap 0) to the 5' end of RNA. In eukaryotes, these terminal cap structures are involved in stabilization, transport, translation of mRNAs. Vaccinia Capping enzyme is composed of two subunits (D1R, D12L), and has three enzymatic activities (RNA triphosphatase, guanylyltransferase by the D1R subunit and guanine methyltransferase by the D12L subunit). All necessary for addition of a complete Cap 0 structure, m7Gppp5'N). In vitro, transcripts can be capped in the presence of reaction buffer, GTP, and the methyl donor (SAM).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e: Recombinant \u003cem\u003eE.coli\u003c\/em\u003e\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 10U\/μL\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit is defined as the amount of enzyme required to incorporate 10pmol of GTP into an 80 nt transcript in 1 hour at 37℃.\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 20mM Tris-HCl (pH 8.0), 0.1mM EDTA, 100mM NaCl, 1mM DTT, 0.1%(v\/v) Triton X-100, 50%(v\/v) glycerol.\u003cbr\u003e\u003cstrong\u003eCompanion Product\u003c\/strong\u003e: \u003ca href=\"https:\/\/shop.hongene.com\/products\/10x-capping-buffer\"\u003e10X Capping Buffer\u003c\/a\u003e, Cat#ON-073, 500mM Tris-HCl, pH 8.0, 50mM KCl, 10mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 10mM DTT; S-adenosylmethionine (SAM), 32mM, ON-074; 10mM GTP, ON-075.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan style=\"background-color: #ffecd9; border-radius: 4px; color: #c62828; padding: 0 5px;\"\u003e\u003csvg viewbox=\"0 0 16 16\" fill=\"currentColor\" height=\"16\" width=\"16\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" style=\"margin: 0 5px -2px 0;\"\u003e\n      \u003cpath d=\"M8 15A7 7 0 1 1 8 1a7 7 0 0 1 0 14m0 1A8 8 0 1 0 8 0a8 8 0 0 0 0 16\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M7.002 11a1 1 0 1 1 2 0 1 1 0 0 1-2 0M7.1 4.995a.905.905 0 1 1 1.8 0l-.35 3.507a.552.552 0 0 1-1.1 0z\"\u003e\u003c\/path\u003e\n    \u003c\/svg\u003eDo not resuspend the RNA in an EDTA-containing solution.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eTo enhance the stability of RNA in solution, RNase inhibitor is desired, which can be add to a final concertation of 1U\/μL at the time of reaction set-up.\u003c\/li\u003e\n\u003cli\u003eSAM is unstable at pH 7-8, 37℃ and should be mixed fresh for each reaction series. We recommend determining how many reactions will be performed and diluting an aliquot of the 32mM stock to 2mM.\u003c\/li\u003e\n\u003cli\u003eHeating the solution of RNA prior to incubation with Vaccinia capping enzyme, to remove secondary structure on the 5' end of the transcripts. For highly structured 5' ends, increase the RNA heat-denaturation conditions used. For example, 65℃ for 20 min, 75℃ for 10 min, 85℃ for 5 min, etc.\u003c\/li\u003e\n\u003cli\u003eVaccinia Capping Enzyme and 2'-O-methyltransferase work together to produce the Cap 1 structure.\u003c\/li\u003e\n\u003cli\u003eCapping reaction incubation time can be increased up to 3 hours at 37℃ for a high capping efficiency for transcripts with known structured 5' ends.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e: -20℃\u003cbr\u003e\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e: Free of Endonuclease, Exonuclease, and RNase activities.\u003cbr\u003e\u003cstrong\u003ePhysical Purity\u003c\/strong\u003e: ＞95% by SDS-PAGE.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003cp\u003e\u003ccite\u003e1. Otto J.P. Kyrieleis, Jonathan Chang, Marcos de la Pena, et al. Structure. 22, 452-465(2014).\u003c\/cite\u003e\u003cbr\u003e\u003ccite\u003e2. Konarska, M.M. et al., (1984) Cell 38, 731.\u003cbr\u003e3. Kuge, H. et al., (1998) Nucl. Acids Res. 26, 3208.\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ Non-GMP \/ 1kU \/ centrifuge tube","offer_id":44545973911752,"sku":"ON-028-KU001-P","price":145.0,"currency_code":"USD","in_stock":true},{"title":"M (Molecular Biology) \/ Non-GMP \/ 10kU \/ centrifuge tube","offer_id":44545973944520,"sku":"ON-028-KU010-P","price":1400.0,"currency_code":"USD","in_stock":true},{"title":"M (Molecular Biology) \/ GMP \/ 1kU \/ centrifuge tube","offer_id":44545973977288,"sku":"ON-028-G","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_b03bba3b-9499-448d-875e-afd6405f99a6.jpg?v=1748588699"},{"product_id":"bspqi","title":"BspQI","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n.product-des-sequence {\n    display: flex;\n    flex-direction: column;\n    justify-items: center;\n    font-size: 25px;\n    margin-bottom: 20px;\n}\n.des-sequence-order {\n    display: flex;\n}\n.sequence-char {\n    text-align: center;\n    width: 25px;\n}\n.sequence-dots {\n    letter-spacing: 6px;\n}\n\n@media (max-width: 450px) {\n.product-des-sequence {\n    font-size: 21px\n}\n.sequence-char {\n    width: 21px;\n}\n}\n.bio-chart {\n    border: solid #3361ac 2px;\n    text-align: center;\n    margin-bottom: 15px;\n    border-radius: 12px;\n    box-shadow: 0px 0px 5px rgba(22, 47, 101, 0.5);\n}\n.bio-chart-title {\n    background-color: #162f65;\n    color: white;\n    display: flex;\n    padding: 5px 0;\n    border-radius: 9px 9px 0 0;\n}\n.bio-chart-row:nth-child(odd) {\n    background-color: #ebf6ff;\n    display: flex;\n    padding: 5px 0;\n}\n.bio-chart-row:nth-child(even) {\n    background-color: white;\n    display: flex;\n    padding: 5px 0;\n}\n.bio-chart-row:last-child {\n    border-radius: 0 0 12px 12px;\n}\n.chart-col-8 {\n    width: 66.67%;\n}\n.chart-col-4 {\n    width: 33.33%;\n}\n.chart-component {\n    color: #3361ac;\n    font-weight: bold;\n}\n.chart-parameter {\n    color: #162f65;\n}\n\u003c\/style\u003e\n\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003eBspQI is a thermostable Type IIS restriction endonuclease (REase) with the recognition sequence 5'-GCTCTTCN1\/N4-3'\u003c\/p\u003e\n\u003cdiv class=\"product-des-sequence\"\u003e\n\u003cdiv class=\"des-sequence-order\"\u003e\n\u003cdiv class=\"sequence-char\"\u003e5'\u003c\/div\u003e\n\u003cdiv class=\"sequence-dots\"\u003e...\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eG\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eC\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eT\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eC\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eT\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eT\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eC\u003c\/div\u003e\n\u003cdiv style=\"margin: 0 5px;\"\u003e(N)\u003csub style=\"font-size: 14px; width: 14px;\"\u003e1\u003c\/sub\u003e↓\n\u003c\/div\u003e\n\u003cdiv class=\"sequence-dots\"\u003e...\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003e3'\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"des-sequence-order\"\u003e\n\u003cdiv class=\"sequence-char\"\u003e3'\u003c\/div\u003e\n\u003cdiv class=\"sequence-dots\"\u003e...\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eC\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eG\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eA\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eG\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eA\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eA\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003eG\u003c\/div\u003e\n\u003cdiv style=\"margin: 0 5px;\"\u003e(N)\u003csub style=\"font-size: 14px; width: 14px;\"\u003e4\u003c\/sub\u003e↑\n\u003c\/div\u003e\n\u003cdiv class=\"sequence-dots\"\u003e...\u003c\/div\u003e\n\u003cdiv class=\"sequence-char\"\u003e5'\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e: Recombinant E.coli\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 10U\/μL\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit is defined as the amount of enzyme required to digest 1μg of λDNA in 1X Cut Buffer following 1 hour incubation at 50°C in a total reaction volume of 50μL.\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 20mM Tris-HCl, 0.1mM EDTA, 500mM KCl, 1mM DTT, 0.1%Triton X-100, 50%(v\/v) glycerol, pH7.0, 25℃.\u003cbr\u003e\u003cstrong\u003eCompanion Product\u003c\/strong\u003e: \u003ca href=\"https:\/\/shop.hongene.com\/products\/10x-cut-buffer\"\u003e10X Cut Buffer\u003c\/a\u003e, Cat#ON-268\/ON-268-G, 500mM Tris-HCl, 1000mM NaCl, 100mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 10mM DTT, pH7.9, 25℃.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eRestriction Digest Protocol\u003c\/strong\u003e:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eOn ice, combine the following reaction components in the order given:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"bio-chart\"\u003e\n\u003cdiv class=\"bio-chart-title\"\u003e\n\u003cdiv class=\"chart-col-8\"\u003eComponent\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4\"\u003eAmount\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003eDNA\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e1μg\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003e10X Cut Buffer\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e5μL\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003eBspQI\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e10 units\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bio-chart-row\"\u003e\n\u003cdiv class=\"chart-col-8 chart-component\"\u003eTotal Reaction Volume\u003c\/div\u003e\n\u003cdiv class=\"chart-col-4 chart-parameter\"\u003e50μL\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003col start=\"2\"\u003e\n\u003cli\u003eIncubate at 50℃, 60min.\u003c\/li\u003e\n\u003cli\u003eHeat inactivity enzyme by incubating at 80℃, 2min.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Sensitivity\u003c\/strong\u003e: Not CpG methylation-sensitive, not dam methylation-sensitive, not dcm methylation-sensitive.\u003cbr\u003e\u003cstrong\u003eEnzyme stability\u003c\/strong\u003e: BspQI half-life period at 50℃ is 2 hours in 1X Cut Buffer.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e: -20℃\u003cbr\u003e\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e: Free of endonuclease, exonuclease and RNase activities.\u003cbr\u003e\u003cstrong\u003ePhysical Purity\u003c\/strong\u003e: ＞95% by SDS-PAGE.\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ Non-GMP \/ 10kU \/ centrifuge tube","offer_id":44579126608072,"sku":"ON-124-KU010-P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"M (Molecular Biology) \/ GMP \/ 10kU \/ centrifuge tube","offer_id":44579126640840,"sku":"ON-124-G","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_5ce49cfb-bba1-408c-8f4b-d7de9770ce58.jpg?v=1748588696"},{"product_id":"t7-rna-polymerase","title":"T7 RNA polymerase","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n.rte-companion-products {\n    display: flex;\n    gap: 10px;\n    margin-bottom: 25px;\n}\n.rte-companion-link {\n    padding: 8px;\n    margin: 5px;\n    width: 50%;\n    border-radius: 8px;\n    box-shadow: 0 0 5px #162f6580;\n    text-decoration: none !important;\n    transition: transform 0.3s;\n}\n.rte-companion-link:hover {\n    transform: translateY(-5px);\n}\n.rte-companion-product {\n    display: flex;\n    gap: 10px;\n}\n.companion-product-img {\n    width: 46%;\n    min-width: 150px;\n    max-width: 220px;\n}\n.companion-product-content {\n    width: 50%;\n}\n.rte-product-catalog, .rte-product-volumn {\n    font-size: 14px;\n    display: block;\n    margin-top: 5px;\n    color: #162f65;\n}\n.rte-product-title {\n    color: #3361ac;\n    font-weight: 700;\n    display: block;\n    margin-bottom: 5px;\n}\n\n.rte-points-operation li {\n    margin-bottom: 1em;\n}\n.rte-points-operation span {\n    display: block;\n    margin-bottom: 0.5em;\n}\n\n@media (max-width: 768px) {\n.rte-companion-products {\n    flex-direction: column;\n}\n.rte-companion-link {\n    width: 95%;\n}\n}\n\u003c\/style\u003e\n\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003eT7 RNA Polymerase is a monomeric bacteriophage encoded DNA directed RNA polymerase which catalyzes the formation of RNA in the 5'-3' direction. In the process of initiation of transcription T7 recognizes a specific promoter sequence, the T7 promoter. T7 consists of 883 amino acids and has a molecular weight of 99kDa.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e: Recombinant E.\u003cem\u003ecoli\u003c\/em\u003e\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 50\/200\/1000U\/μL (KU\/mL)\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of rATP into acid-insoluble product in 1 hour at 37℃.\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 20mM Tris-HCl PH 7.9, 100mM NaCl, 10mM DTT, 0.1% Triton X-100, 1mM EDTA, 50%(v\/v) Glycerol.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompanion Products:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"rte-companion-products\"\u003e\n\u003ca title=\"jump to ON-062\" href=\"https:\/\/shop.hongene.com\/products\/10x-ivt-reaction-buffer\" class=\"rte-companion-link\"\u003e\n\u003cdiv class=\"rte-companion-product\"\u003e\n\u003cdiv class=\"companion-product-img\"\u003e\u003csvg xml:space=\"preserve\" style=\"enable-background: new 0 0 600 600; border-radius: 8px; border: solid 1px #162f65;\" viewbox=\"0 0 600 600\" y=\"0px\" x=\"0px\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" version=\"1.1\"\u003e\n        \u003cstyle type=\"text\/css\"\u003e\n\t.st0{fill:#BFBFBF;}\n\t.st1{fill:none;stroke:#bfbfbf;stroke-width:21.1099;stroke-miterlimit:10;}\n\t.st2{fill:none;stroke:#bfbfbf;stroke-width:24.3757;stroke-miterlimit:10;}\n\u003c\/style\u003e\n        \u003cg\u003e\n          \u003cpath d=\"M282.4,390.4c-9.79,0-17.75,7.96-17.75,17.75c0,9.79,7.96,17.76,17.75,17.76c9.8,0,17.76-7.97,17.76-17.76\n\t\tC300.17,398.37,292.21,390.4,282.4,390.4z M282.4,418.81c-3.8,0-7.32-2.03-9.22-5.33c-1.9-3.3-1.9-7.36,0-10.65\n\t\tc1.91-3.3,5.43-5.33,9.23-5.33c5.89,0.01,10.65,4.78,10.65,10.66C293.07,414.05,288.3,418.81,282.4,418.81z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M300,446.01c-6.59,0-11.94,5.36-11.94,11.94c0,6.59,5.36,11.95,11.94,11.95c6.59,0,11.95-5.36,11.95-11.95\n\t\tC311.94,451.37,306.59,446.01,300,446.01z M300,465.12c-2.56,0-4.92-1.37-6.2-3.59c-1.28-2.22-1.28-4.95,0-7.17\n\t\tc1.29-2.22,3.65-3.59,6.21-3.59c3.96,0.01,7.17,3.22,7.17,7.17C307.17,461.91,303.96,465.12,300,465.12z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M311.34,314.76c-14.11,0-25.58,11.47-25.58,25.58c0,14.11,11.47,25.6,25.58,25.6c14.12,0,25.6-11.49,25.6-25.6\n\t\tC336.94,326.23,325.47,314.76,311.34,314.76z M311.34,355.7c-5.48,0-10.55-2.93-13.29-7.68c-2.74-4.75-2.74-10.6,0-15.35\n\t\tc2.75-4.75,7.82-7.68,13.3-7.68c8.49,0.01,15.35,6.89,15.35,15.37C326.71,348.83,319.83,355.7,311.34,355.7z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M357.17,132.88v312.25c0,0.3,0,0.58-0.02,0.88c-0.46,31.17-25.88,56.29-57.16,56.29s-56.7-25.12-57.16-56.29\n\t\tc-0.02-0.3-0.02-0.58-0.02-0.88V132.88H357.17z\" class=\"st1\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M299.99,445.13c0,0.3,0,0.58,0.02,0.88h-0.02V445.13z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M300.01,445.13v0.88h-0.02C300.01,445.71,300.01,445.43,300.01,445.13z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M366.41,132.88H233.59c-9.68,0-17.59-7.92-17.59-17.59v0c0-9.68,7.92-17.59,17.59-17.59h132.82\n\t\tc9.68,0,17.59,7.92,17.59,17.59v0C384,124.96,376.08,132.88,366.41,132.88z\" class=\"st2\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M242.84,257.19c0,0,79.13,35.97,114.33-40.66\" class=\"st1\"\u003e\u003c\/path\u003e\n        \u003c\/g\u003e\n      \u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv class=\"companion-product-content\"\u003e\n\u003cspan class=\"rte-product-catalog\"\u003eCat#ON-062\u003c\/span\u003e \u003cspan class=\"rte-product-title\"\u003e10X IVT Reaction Buffer\u003c\/span\u003e \u003cspan class=\"rte-product-volumn\"\u003e\u003cspan style=\"font-size: 12px;\"\u003einside with:\u003c\/span\u003e\u003cbr\u003e- 400mM Tris PH7.9\u003cbr\u003e- 100mM DTT\u003cbr\u003e- 20mM Spermidine\u003cbr\u003e- 60mM MgCl\u003csub\u003e2\u003c\/sub\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\u003ca title=\"jump to ON-041\" href=\"https:\/\/shop.hongene.com\/products\/10x-ivt-reaction-buffer-without-mg2\" class=\"rte-companion-link\"\u003e\n\u003cdiv class=\"rte-companion-product\"\u003e\n\u003cdiv class=\"companion-product-img\"\u003e\u003csvg xml:space=\"preserve\" style=\"enable-background: new 0 0 600 600; border-radius: 8px; border: solid 1px #162f65;\" viewbox=\"0 0 600 600\" y=\"0px\" x=\"0px\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" version=\"1.1\"\u003e\n        \u003cstyle type=\"text\/css\"\u003e\n            .st0{fill:#BFBFBF;}\n            .st1{fill:none;stroke:#bfbfbf;stroke-width:21.1099;stroke-miterlimit:10;}\n            .st2{fill:none;stroke:#bfbfbf;stroke-width:24.3757;stroke-miterlimit:10;}\n        \u003c\/style\u003e\n        \u003cg\u003e\n          \u003cpath d=\"M282.4,390.4c-9.79,0-17.75,7.96-17.75,17.75c0,9.79,7.96,17.76,17.75,17.76c9.8,0,17.76-7.97,17.76-17.76\n\t\tC300.17,398.37,292.21,390.4,282.4,390.4z M282.4,418.81c-3.8,0-7.32-2.03-9.22-5.33c-1.9-3.3-1.9-7.36,0-10.65\n\t\tc1.91-3.3,5.43-5.33,9.23-5.33c5.89,0.01,10.65,4.78,10.65,10.66C293.07,414.05,288.3,418.81,282.4,418.81z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M300,446.01c-6.59,0-11.94,5.36-11.94,11.94c0,6.59,5.36,11.95,11.94,11.95c6.59,0,11.95-5.36,11.95-11.95\n\t\tC311.94,451.37,306.59,446.01,300,446.01z M300,465.12c-2.56,0-4.92-1.37-6.2-3.59c-1.28-2.22-1.28-4.95,0-7.17\n\t\tc1.29-2.22,3.65-3.59,6.21-3.59c3.96,0.01,7.17,3.22,7.17,7.17C307.17,461.91,303.96,465.12,300,465.12z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M311.34,314.76c-14.11,0-25.58,11.47-25.58,25.58c0,14.11,11.47,25.6,25.58,25.6c14.12,0,25.6-11.49,25.6-25.6\n\t\tC336.94,326.23,325.47,314.76,311.34,314.76z M311.34,355.7c-5.48,0-10.55-2.93-13.29-7.68c-2.74-4.75-2.74-10.6,0-15.35\n\t\tc2.75-4.75,7.82-7.68,13.3-7.68c8.49,0.01,15.35,6.89,15.35,15.37C326.71,348.83,319.83,355.7,311.34,355.7z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M357.17,132.88v312.25c0,0.3,0,0.58-0.02,0.88c-0.46,31.17-25.88,56.29-57.16,56.29s-56.7-25.12-57.16-56.29\n\t\tc-0.02-0.3-0.02-0.58-0.02-0.88V132.88H357.17z\" class=\"st1\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M299.99,445.13c0,0.3,0,0.58,0.02,0.88h-0.02V445.13z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M300.01,445.13v0.88h-0.02C300.01,445.71,300.01,445.43,300.01,445.13z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M366.41,132.88H233.59c-9.68,0-17.59-7.92-17.59-17.59v0c0-9.68,7.92-17.59,17.59-17.59h132.82\n\t\tc9.68,0,17.59,7.92,17.59,17.59v0C384,124.96,376.08,132.88,366.41,132.88z\" class=\"st2\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M242.84,257.19c0,0,79.13,35.97,114.33-40.66\" class=\"st1\"\u003e\u003c\/path\u003e\n        \u003c\/g\u003e\n      \u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv class=\"companion-product-content\"\u003e\n\u003cspan class=\"rte-product-catalog\"\u003eCat#ON-041\u003c\/span\u003e \u003cspan class=\"rte-product-title\"\u003e10X IVT Reaction Buffer without Mg\u003csup\u003e2+\u003c\/sup\u003e\u003c\/span\u003e \u003cspan class=\"rte-product-volumn\"\u003e\u003cspan style=\"font-size: 12px;\"\u003einside with:\u003c\/span\u003e\u003cbr\u003e- 400mM Tris PH7.9\u003cbr\u003e- 100mM DTT\u003cbr\u003e- 20mM Spermidine.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\n\u003c\/div\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003col class=\"rte-points-operation\"\u003e\n\u003cli\u003eT7 RNA Polymerase is extremely sensitive to salt inhibition. For best overall salt concentration should not exceed 50mM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eHigher yields of RNA may be obtained by raising NTP concentrations (up to 10mM each).\u003c\/span\u003e \u003cspan\u003eMg\u003csup\u003e2+\u003c\/sup\u003e concentration should be raised to 4~10mM above the total NTP concentration.\u003c\/span\u003e \u003cspan\u003eT7 RNA Polymerase should be raised to 30K units\/mL.\u003c\/span\u003e \u003cspan\u003eAdditionally, inorganic pyrophosphatase should be added to a final concentration of 4 units\/mL.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eAn apparent decrease in enzyme activity over time may be due to the breakdown of dithiothreitol in the reaction buffer; even when stored at –20°C.\u003c\/span\u003e \u003cspan\u003eIf you observe a decrease in yield, try supplementing your reactions with a final concentration of 20mM fresh dithiothreitol.\u003c\/span\u003e \u003cspan\u003eOr make a reaction buffer without DTT and add fresh DTT when preparing reaction mixture.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e: -20℃\u003cbr\u003e\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e: Free of endonuclease, exonuclease and RNase activities.\u003cbr\u003e\u003cstrong\u003ePhysical Purity\u003c\/strong\u003e: ＞95% by SDS-PAGE.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003cp\u003e\u003ccite\u003e1. Chamberlin, M., McGrath, J. \u0026amp; Waskell, L. Nature 228, 227–31 (1970).\u003c\/cite\u003e\u003cbr\u003e\u003ccite\u003e2. Davanloo, P., Rosenberg, A. H., Dunn, J. J. \u0026amp; Studier, F. W. Proc. Natl. Acad. Sci. U. S. A. 81, 2035–9 (1984).\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"Non-GMP \/ 50 kU\/mL \/ 10kU \/ centrifuge tube","offer_id":44579126706376,"sku":"ON-004-C050-KU010-P","price":25.0,"currency_code":"USD","in_stock":true},{"title":"Non-GMP \/ 50 kU\/mL \/ 50kU \/ centrifuge tube","offer_id":44579126739144,"sku":"ON-004-C050-KU050-P","price":115.0,"currency_code":"USD","in_stock":true},{"title":"Non-GMP \/ 200 kU\/mL \/ 100kU \/ centrifuge tube","offer_id":44579126771912,"sku":"ON-004-C200-KU100-P","price":230.0,"currency_code":"USD","in_stock":true},{"title":"Non-GMP \/ 200 kU\/mL \/ 200kU \/ centrifuge tube","offer_id":44579126804680,"sku":"ON-004-C200-KU200-P","price":465.0,"currency_code":"USD","in_stock":true},{"title":"Non-GMP \/ 1000 kU\/mL \/ 100kU \/ centrifuge tube","offer_id":44579126837448,"sku":"ON-004-C1000-KU100-P","price":235.0,"currency_code":"USD","in_stock":true},{"title":"Non-GMP \/ 1000 kU\/mL \/ 1000kU \/ centrifuge tube","offer_id":44579126870216,"sku":"ON-004-C1000-KU1000-P","price":1200.0,"currency_code":"USD","in_stock":true},{"title":"GMP \/ 50 kU\/mL \/ 50kU \/ centrifuge tube","offer_id":44579126902984,"sku":"ON-004-G","price":0.0,"currency_code":"USD","in_stock":true},{"title":"GMP \/ 200 kU\/mL \/ 200kU \/ centrifuge tube","offer_id":44579126935752,"sku":"ON-004-C200-G","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_142b3377-1de7-426f-ab85-34410c5c3bd5.jpg?v=1748588695"},{"product_id":"dnase-i-rnase-free","title":"DNase I (RNase-free)","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n.rte-companion-products {\n    display: flex;\n    gap: 10px;\n    margin-bottom: 25px;\n}\n.rte-companion-link {\n    padding: 8px;\n    margin: 5px;\n    max-width: 450px;\n    border-radius: 8px;\n    box-shadow: 0 0 5px #162f6580;\n    text-decoration: none !important;\n    transition: transform 0.3s;\n}\n.rte-companion-link:hover {\n    transform: translateY(-5px);\n}\n.rte-companion-product {\n    display: flex;\n    gap: 10px;\n}\n.companion-product-img {\n    width: 46%;\n    min-width: 150px;\n    max-width: 220px;\n}\n.companion-product-content {\n    width: 50%;\n}\n.rte-product-catalog, .rte-product-volumn {\n    font-size: 14px;\n    display: block;\n    margin-top: 5px;\n    color: #162f65;\n}\n.rte-product-title {\n    color: #3361ac;\n    font-weight: 700;\n    display: block;\n    margin-bottom: 5px;\n}\n.rte-points-operation li {\n    margin-bottom: 1em;\n}\n.rte-points-operation span {\n    display: block;\n    margin-bottom: 0.5em;\n}\n\n@media (max-width: 768px) {\n.rte-companion-products {\n    flex-direction: column;\n}\n.rte-companion-link {\n    width: 95%;\n}\n}\n\u003c\/style\u003e\n\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003eDNase I (RNase free) is an endonuclease that digests single- and double-stranded DNA. It nonspecifically cleaves DNA to release di-,tri- and oligonucleotide products with 5'-phosphorylated and 3'-hydroxylated end.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e: Recombinant E.coli\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 1U\/μL\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit is defined as the amount of enzyme required to completely degrade 1μg of λDNA in 10 minutes at 37℃.\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 50mM Tris-HCl (pH7.5, 25℃), 10mM CaCl\u003csub\u003e2\u003c\/sub\u003e and 50% Glyercol\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompanion Product:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"rte-companion-products\"\u003e\u003ca title=\"jump to ON-077\" href=\"https:\/\/shop.hongene.com\/products\/10x-dnase-i-reaction-buffer\" class=\"rte-companion-link\"\u003e\n\u003cdiv class=\"rte-companion-product\"\u003e\n\u003cdiv class=\"companion-product-img\"\u003e\u003csvg xml:space=\"preserve\" style=\"enable-background: new 0 0 600 600; border-radius: 8px; border: solid 1px #162f65;\" viewbox=\"0 0 600 600\" y=\"0px\" x=\"0px\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" version=\"1.1\"\u003e\n        \u003cstyle type=\"text\/css\"\u003e\n\t.st0{fill:#BFBFBF;}\n\t.st1{fill:none;stroke:#bfbfbf;stroke-width:21.1099;stroke-miterlimit:10;}\n\t.st2{fill:none;stroke:#bfbfbf;stroke-width:24.3757;stroke-miterlimit:10;}\n\u003c\/style\u003e\n        \u003cg\u003e\n          \u003cpath d=\"M282.4,390.4c-9.79,0-17.75,7.96-17.75,17.75c0,9.79,7.96,17.76,17.75,17.76c9.8,0,17.76-7.97,17.76-17.76\n\t\tC300.17,398.37,292.21,390.4,282.4,390.4z M282.4,418.81c-3.8,0-7.32-2.03-9.22-5.33c-1.9-3.3-1.9-7.36,0-10.65\n\t\tc1.91-3.3,5.43-5.33,9.23-5.33c5.89,0.01,10.65,4.78,10.65,10.66C293.07,414.05,288.3,418.81,282.4,418.81z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M300,446.01c-6.59,0-11.94,5.36-11.94,11.94c0,6.59,5.36,11.95,11.94,11.95c6.59,0,11.95-5.36,11.95-11.95\n\t\tC311.94,451.37,306.59,446.01,300,446.01z M300,465.12c-2.56,0-4.92-1.37-6.2-3.59c-1.28-2.22-1.28-4.95,0-7.17\n\t\tc1.29-2.22,3.65-3.59,6.21-3.59c3.96,0.01,7.17,3.22,7.17,7.17C307.17,461.91,303.96,465.12,300,465.12z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M311.34,314.76c-14.11,0-25.58,11.47-25.58,25.58c0,14.11,11.47,25.6,25.58,25.6c14.12,0,25.6-11.49,25.6-25.6\n\t\tC336.94,326.23,325.47,314.76,311.34,314.76z M311.34,355.7c-5.48,0-10.55-2.93-13.29-7.68c-2.74-4.75-2.74-10.6,0-15.35\n\t\tc2.75-4.75,7.82-7.68,13.3-7.68c8.49,0.01,15.35,6.89,15.35,15.37C326.71,348.83,319.83,355.7,311.34,355.7z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M357.17,132.88v312.25c0,0.3,0,0.58-0.02,0.88c-0.46,31.17-25.88,56.29-57.16,56.29s-56.7-25.12-57.16-56.29\n\t\tc-0.02-0.3-0.02-0.58-0.02-0.88V132.88H357.17z\" class=\"st1\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M299.99,445.13c0,0.3,0,0.58,0.02,0.88h-0.02V445.13z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M300.01,445.13v0.88h-0.02C300.01,445.71,300.01,445.43,300.01,445.13z\" class=\"st0\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M366.41,132.88H233.59c-9.68,0-17.59-7.92-17.59-17.59v0c0-9.68,7.92-17.59,17.59-17.59h132.82\n\t\tc9.68,0,17.59,7.92,17.59,17.59v0C384,124.96,376.08,132.88,366.41,132.88z\" class=\"st2\"\u003e\u003c\/path\u003e\n          \u003cpath d=\"M242.84,257.19c0,0,79.13,35.97,114.33-40.66\" class=\"st1\"\u003e\u003c\/path\u003e\n        \u003c\/g\u003e\n      \u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv class=\"companion-product-content\"\u003e\n\u003cdiv class=\"rte-product-catalog\"\u003eCat#ON-077\u003c\/div\u003e\n\u003cdiv class=\"rte-product-title\"\u003e10X DNase I Reaction Buffer\u003c\/div\u003e\n\u003cdiv class=\"rte-product-volumn\"\u003e\n\u003cdiv style=\"font-size: 12px; margin-bottom: 5px;\"\u003einside with:\u003c\/div\u003e\n\u003cdiv\u003e- 100mM Tris-HCl pH7.6, 25℃\u003cbr\u003e- 25mM MgCl\u003csub\u003e2\u003c\/sub\u003e\u003cbr\u003e- 5mM CaCl\u003csub\u003e2\u003c\/sub\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eDNase I treatment after IVT reaction:\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eDirectly add DNase I (1unit DNase I per ug of DNA template) to IVT Reaction, mix well and incubate 30 min at 37°C.\u003c\/li\u003e\n\u003cli\u003eAdd 0.5M EDTA to a final concertation over one millimole than divalent cations.\u003c\/li\u003e\n\u003cli\u003eHeat inactivate at 75℃ for 10min.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e: -20℃\u003cbr\u003e\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e: Free of RNase activities.\u003cbr\u003e\u003cstrong\u003ePhysical Purity\u003c\/strong\u003e: ＞95% by SDS-PAGE.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003cp\u003e\u003ccite\u003e1. A F Worrall，B A Connolly, J，Biol，Chem. 265,21889–21896 (1990).\u003c\/cite\u003e\u003cbr\u003e\u003ccite\u003e2. Ching-Ying Chen，Shao-Chun Lu，et al. Gene. 206, 181-184(1998).\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ Non-GMP \/ 1kU \/ centrifuge tube","offer_id":44579127066824,"sku":"ON-109-KU001-P","price":25.0,"currency_code":"USD","in_stock":true},{"title":"M (Molecular Biology) \/ GMP \/ 1kU \/ centrifuge tube","offer_id":44579127099592,"sku":"ON-109-G","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_bdbb30ba-9665-4b52-a90c-cd69b496f14f.jpg?v=1748588694"},{"product_id":"pyrophosphatase-inorganic-yeast","title":"Pyrophosphatase Inorganic (yeast)","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n\u003c\/style\u003e\n\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003ePPase is prepared from an E. coli strain containing Saccharomyces cerevisiae ppa gene. It is a homodimer consisting of two equal subunits of molecular weight 32-35kDa. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e: Recombinant \u003cem\u003eE.coli\u003c\/em\u003e\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 0.1U\/μL\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25℃ in 100mM Tris-HCl, [pH 7.2], 2mM MgCl\u003csub\u003e2\u003c\/sub\u003e and 2mM PPi in a reaction volume of 0.5mL)\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 20mM Tris-HCl PH8.0, 1mM DTT, 0.1mM EDTA, 50% Glycerol.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003cp\u003eWhen in vitro transcription, higher yields of RNA may be obtained by adding inorganic pyrophosphatase to a final concentration of 4units\/mL.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e: -20℃\u003cbr\u003e\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e: Free of endonuclease, exonuclease and RNase activities.\u003cbr\u003e\u003cstrong\u003ePhysical Purity\u003c\/strong\u003e: ＞95% by SDS-PAGE.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003cp\u003e\u003ccite\u003e1. Cooperman, B.S., The mechanism of action of yeast inorganic pyrophosphatase, Meth. Enzymol., 87, 526-548, 1982.\u003c\/cite\u003e\u003cbr\u003e\u003ccite\u003e2. Cunningham, P.R. and Ofengand, J., Use of inorganic pyrophosphatase to improve the yield of in vitro transcription reactions catalyzed by T7 RNA polymerase, Biotechniques, 9, 713-714, 1990.\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ Non-GMP \/ 0.1kU \/ centrifuge tube","offer_id":44579127165128,"sku":"ON-025-U100-P","price":128.0,"currency_code":"USD","in_stock":true},{"title":"M (Molecular Biology) \/ GMP \/ 0.1kU \/ centrifuge tube","offer_id":44579127197896,"sku":"ON-025-G","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_3b3201ee-7ae5-4807-9b90-4c7287132911.jpg?v=1748588693"},{"product_id":"rnase-inhibitor","title":"RNase Inhibitor","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n.operation-alert {\n    background-color: #ffecd9;\n    border-radius: 8px;\n    padding: 0.5em 1em;\n    margin: 0 5px;\n    display: flex;\n    gap: 0.25em;\n    color: #c62828;\n    box-shadow: 0 0 5px rgba(22, 47, 101, 0.3);\n    margin-bottom: 20px;\n}\n.operation-alert-icon {\n    margin-top: 3px;\n}\n\u003c\/style\u003e\n\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003eRibonuclease Inhibitor, Human Placenta is a recombinant human placental protein with specifically inhibits broad-spectrum RNase such as RNase A, RNase B and RNase C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H, Taq DNA polymerase, M-MLV Reverse Transcriptase and T7 RNA Polymerase. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases at 1:1 ratio. The Ki value for binding of Ribonuclease Inhibitor, Human Placenta to RNase is approximately 10\u003csup\u003e-14\u003c\/sup\u003eM.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource\u003c\/strong\u003e: Recombinant \u003cem\u003eE.coli\u003c\/em\u003e\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 40U\/μL\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit is defined as the amount of Recombinant Ribonuclease Inhibitor required inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2',3'-cyclic monophosphate by ribonuclease A. \u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 20mM HEPES-KOH (pH7.6), 50mM KCl, 8mM DTT, 50%(v\/v) glycerol.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eRibonuclease Inhibitor is active over a broad pH range (pH5.5-9).\u003c\/li\u003e\n\u003cli\u003eRibonuclease Inhibitor is used in the standard RT and in vitro transcription, and the final concentration is 1U\/μL.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"operation-alert\"\u003e\n\u003cdiv class=\"operation-alert-icon\"\u003e\u003csvg viewbox=\"0 0 16 16\" fill=\"currentColor\" height=\"16\" width=\"16\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"\u003e\n      \u003cpath d=\"M8 15A7 7 0 1 1 8 1a7 7 0 0 1 0 14m0 1A8 8 0 1 0 8 0a8 8 0 0 0 0 16\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M7.002 11a1 1 0 1 1 2 0 1 1 0 0 1-2 0M7.1 4.995a.905.905 0 1 1 1.8 0l-.35 3.507a.552.552 0 0 1-1.1 0z\"\u003e\u003c\/path\u003e\n    \u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv\u003eTo prevent the release of active ribonuclease, temperatures greater than 50℃ and high concentrations of urea or other denaturing agents should be avoided.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e: -20℃\u003cbr\u003e\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e: Free of endonuclease, exonuclease, Nickase, RNase and latent RNase activities.\u003cbr\u003e\u003cstrong\u003ePhysical Purity\u003c\/strong\u003e: ＞95% by SDS-PAGE.\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003cp\u003e\u003ccite\u003e1. Blackburn, P., Wilson, G. and Moore,S. J. Biol. Chem. 252, 5904-10(1977).\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ Non-GMP \/ 40kU \/ centrifuge tube","offer_id":44579127361736,"sku":"ON-039-KU040-P","price":325.0,"currency_code":"USD","in_stock":true},{"title":"M (Molecular Biology) \/ GMP \/ 40kU \/ centrifuge tube","offer_id":44579127394504,"sku":"ON-039-G","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_4a783684-19f1-47dc-b2cc-941968b3d5c5.jpg?v=1748588691"},{"product_id":"s-adenosylmethionine-sam","title":"S-adenosylmethionine (SAM)","description":"\u003cp\u003eS-Adenosyl-L-Methionine (SAM) serves as the essential active methyl donor for methyltransferase-driven reactions. Our product portfolio supports a wide range of applications, including fundamental epigenetic research, in-vitro mRNA capping, enzymatic assay kit development, and biocatalysis for synthetic biology.\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ Non-GMP \/ 1mL \/ centrifuge tube","offer_id":44579127558344,"sku":"ON-074-ML001-P","price":16.0,"currency_code":"USD","in_stock":true},{"title":"M (Molecular Biology) \/ GMP \/ 1mL \/ centrifuge tube","offer_id":44579127591112,"sku":"ON-074-G","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P2_88efd80d-e402-4e58-9c51-077b17578e03.jpg?v=1748588690"},{"product_id":"high-purity-5x-ivt-reaction-buffer","title":"High Purity 5x IVT Reaction Buffer","description":"\u003cp class=\"MsoNormal\" style=\"text-align: center;\"\u003e\u003cspan\u003eHigh\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Cambria Math;\"\u003e‑\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003ePurity 5\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: 宋体;\"\u003e×\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e IVT Reaction Buffer, optimized specifically for in\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Cambria Math;\"\u003e‑\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003evitro transcription.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ 1mL \/ centrifuge tube","offer_id":44640736149704,"sku":"ON-298-ML001-P","price":27.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P2_a81eab67-bb88-462e-9f62-2914c734b5c4.jpg?v=1748588678"},{"product_id":"faustovirus-capping-enzyme","title":"Faustovirus capping enzyme","description":"\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eProduct description\u003c\/strong\u003e: Faustavirus capping enzyme (FCE) is encoded by Faustavirus. FCE is a single-subunit enzyme that possesses the three enzymatic activities required for capping RNA with a Cap-0 structure: triphosphatase, guanylyltransferase, and (guanine-N7)-methyltransferase. FCE has a wider reaction temperature range compared to vaccinia capping enzyme (VCE). It can perform capping at temperatures ranging from 37°C to 50°C. FCE acts synergistically with mRNA Cap 2'-O-Methyltransferase to add a Cap-1 structure to mRNA in a single step.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSource\u003c\/strong\u003e: An E. coli strain that carries FCE gene.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e: 25 units\/µL\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e: One unit is defined as the amount of enzyme that converts 25pmols of a 80nt transcript to Cap-0 RNA in 30minutes at 37°C.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e: 40mM Tris-HCl, 100mM NaCl, 50mM Arginine, 0.1mM TCEP, 50% Glycerol, (pH8.0@25°C).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eCompanion Product\u003c\/strong\u003e: 10X Capping Buffer, Cat#ON-073, 500mM Tris-HCl, 50mM KCl, 10mM MgCl2, 10mM DTT, pH8.0@25°C.\u003cbr\u003eS-adenosylmethionine (SAM), 32mM, Cat#ON-074.\u003c\/p\u003e\n\u003ch3\u003eKey points of operation\u003c\/h3\u003e\n\u003cp\u003e1. Do not oscillate vigorously when mixing.\u003cbr\u003e\u003cbr\u003e2. If the reagents, tubes, or micropipette tips are contaminated with RNase, it will result in reduced RNA yield or degradation. Precautions should be taken to avoid RNase contamination during experiments, such as wearing disposable gloves and using special pipettes.\u003cbr\u003e\u003cbr\u003e3. The mRNA solution was heated at 65ºC for 5 minutes to open the secondary structure at the 5' end of the transcription product before warming with FCE. For transcription products with highly structured 5' ends, the time can be extended to 10 minutes or the denaturation temperature can be increased appropriately.\u003cbr\u003e\u003cbr\u003e4. SAM is unstable at pH 7-8 and 37ºC. It is recommended to prepare fresh working solution according to the actual number of reactions and place it on ice before the start of the reaction to prevent SAM degradation.\u003c\/p\u003e\n\u003ch3\u003eSecurity Information\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e: -15 ~ -25℃\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eQuality Assurance\u003c\/strong\u003e: Free of Endonuclease, Exonuclease and RNase activities.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePhysical Purity\u003c\/strong\u003e: \u0026gt;95% by SDS-PAGE.\u003c\/p\u003e\n\u003chr style=\"background: linear-gradient(to right, white , #162F65); border: 0; height: 1px;\"\u003e\n\u003ch3\u003eReference\u003c\/h3\u003e\n\u003cp\u003e\u003ccite\u003e1. Ramanathan, A. et al. (2016). Nucleic Acids Res . 44 (16), 7511–7526.\u003c\/cite\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ccite\u003e2. Furuichi, Y., LaFiandra, A. \u0026amp; Shatkin, A. (1977). Nature. 266, 235-239.\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology)","offer_id":44713002795208,"sku":"ON-981-C025","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_cf554ec8-96cb-43c6-bea1-b1550270cc8e.jpg?v=1748588757"},{"product_id":"t7-rna-polymerase-high-concentration","title":"T7 RNA Polymerase, high concentration","description":"","brand":"Hongene","offers":[{"title":"M (Molecular Biology)","offer_id":44713026289864,"sku":"ON-005","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_526f1621-c098-4b0c-8aba-973e2068c0de.jpg?v=1753079086"},{"product_id":"hixt7","title":"HiXT7","description":"\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003eHiXT7 RNA Polymerase is a DNA-dependent RNA polymerase designed to increase co-transcriptional capping efficiency and reduce double-stranded RNA formation during IVT, enabling the production of high-quality, clean mRNA transcripts. This optimized enzyme is specifically suited for use with HiXCap E1, HiXCap E2, HiXCap E3 and M6 GAAG Cap2 cap analogs, delivering superior 5’-end capping performance compared with wild-type T7 RNA polymerase. The enzyme is fully compatible with 5×HIC IVT Reaction Buffer, and supports up to 100 µg of high-quality capped RNA per reaction.\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ GMP\/Non-GMP","offer_id":45196823331016,"sku":"ON-1078","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_09ec0dff-92a0-4a32-85f0-4504786fce9a.jpg?v=1787119154"}],"url":"https:\/\/shop.hongene.com\/collections\/enzyme-related-buffer-for-mrna.oembed?page=2","provider":"Hongene Online Store","version":"1.0","type":"link"}