{"product_id":"t3-dna-ligase","title":"T3 DNA ligase","description":"\u003ch3\u003eProduct Information\u003c\/h3\u003e\n\u003cp\u003eT3 DNA Ligase is an ATP-dependent dsDNA ligase derived from bacteriophage T3 and recombinantly expressed in \u003ci\u003eE. coli\u003c\/i\u003e. It catalyzes the formation of phosphodiester bonds between adjacent 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA (dsDNA), with higher efficiency for A\/T overhangs than C\/G overhangs. The enzyme efficiently ligates dsDNA with either sticky or blunt ends, repairs nicks in dsDNA, and seals nicks in DNA\/RNA hybrids containing an intact DNA strand. Consequently, T3 DNA Ligase can be used to generate DNA-RNA chimeras and to ligate RNA fragments using a DNA template.\u003c\/p\u003e\n\u003cp\u003eSimilar to T4 DNA Ligase, the addition of PEG 6000 significantly enhances blunt-end ligation efficiency. T3 DNA Ligase exhibits twice the salt tolerance of T4 DNA Ligase, retaining \u0026gt;95% activity in 1.0 M NaCl or KCl, making it an ideal choice for ligation under high-salt conditions.\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ Non-GMP","offer_id":45234134515912,"sku":"ON-1188","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_a15bf9d5-4da8-47ba-b91d-c52f83b98c4b.jpg?v=1792140685","url":"https:\/\/shop.hongene.com\/products\/t3-dna-ligase","provider":"Hongene Online Store","version":"1.0","type":"link"}