{"product_id":"5-me-dmt-2-o-moe-c-bz-ce-phosphoramidite","title":"5-Me-DMT-2'-O-MOE-C(Bz)-CE 亚磷酰胺","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n.rte-des-lists {\n    display: flex;\n    flex-wrap: wrap;\n    gap: 25px;\n    padding: 0 10px;\n    margin-bottom: 25px;\n}\n.rte-list-card {\n    flex: 1;\n    background: rgba(239, 238, 236, 0.3);\n    border-radius: 10px;\n    padding: 20px;\n    box-shadow: 0px 3px 10px rgba(22, 47, 101, 0.2);\n    transition: transform 0.3s;\n    min-width: 290px;\n    position: relative;\n}\n.rte-list-card:hover {\n    transform: translateY(-5px);\n}\n.rte-list-icon {\n    width: 25px;\n    height: 25px;\n    margin: 0px 5px -5px 0px;\n    fill: currentColor;\n}\n.rte-list-card h3 {\n    font-size: 1.2em;\n    padding-bottom: 8px;\n    margin-bottom: 8px;\n    color: #3361ac;\n}\n.rte-dashed-list-title {\n    border-bottom: dashed rgba(193, 212, 231);\n}\n.rte-list-feature {\n    border-left: solid 3px #3361ac80;\n    padding: 3px 0 3px 10px;\n    margin: 0 5px 5px 0;\n}\n.rte-list-feature svg {\n    width: 10px;\n    height: 10px;\n    margin-bottom: -5px;\n    fill: #3361ac80;\n}\n\u003c\/style\u003e\n\u003cp\u003e5-Me-DMT-2'-O-MOE-C(Bz)-CE-Phosphoramidite 是一种 2'-MOE 亚磷酰胺单体，用于固相寡核苷酸合成。它含有用于 5'-羟基保护的 5'-二甲氧基三苯甲基 (DMT) 保护基团，在 5-甲基胞嘧啶上有一个苯甲酰 (Bz) 碱基保护基团，在亚磷酸酯上有一个氰乙基 (CE) 保护基团，以及一个 \u003cstrong\u003e2'-O-甲氧基乙基 (MOE)\u003c\/strong\u003e 修饰。\u003c\/p\u003e\n\u003cp\u003e与未修饰的 RNA 相比，2'-O-MOE 具有\u003cstrong\u003e增强的核酸酶抗性、双链稳定性以及低细胞毒性\u003c\/strong\u003e。\u003c\/p\u003e\n\u003cp\u003e在结构上，核糖的 2'-羟基被 –O–CH₂–CH₂–O–CH₃（甲氧基乙基）基团取代，这使得糖锁定在 RNA 样 C3'-内型构象中，并稳定与互补 RNA 链的杂交形成。\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003e应用\u003c\/h3\u003e\n\u003cdiv class=\"rte-des-lists\"\u003e\n\n\u003cdiv class=\"rte-list-card\"\u003e\n\n\u003ch3 class=\"rte-dashed-list-title\"\u003e\n\n\u003csvg class=\"rte-list-icon\" viewbox=\"0 0 640 640\"\u003e\n        \u003cpath fill=\"currentColor\" d=\"M128 176C128 149.5 149.5 128 176 128C202.5 128 224 149.5 224 176L224 288L128 288L128 176zM240 432C240 383.3 258.1 338.8 288 305L288 176C288 114.1 237.9 64 176 64C114.1 64 64 114.1 64 176L64 464C64 525.9 114.1 576 176 576C213.3 576 246.3 557.8 266.7 529.7C249.7 501.1 240 467.7 240 432zM304.7 499.4C309.3 508.1 321 509.1 328 502.1L502.1 328C509.1 321 508.1 309.3 499.4 304.7C479.3 294 456.4 288 432 288C352.5 288 288 352.5 288 432C288 456.3 294 479.3 304.7 499.4zM361.9 536C354.9 543 355.9 554.7 364.6 559.3C384.7 570 407.6 576 432 576C511.5 576 576 511.5 576 432C576 407.7 570 384.7 559.3 364.6C554.7 355.9 543 354.9 536 361.9L361.9 536z\"\u003e \u003c\/path\u003e\n      \u003c\/svg\u003e RNase H 依赖的反义寡核苷酸 (ASO)\u003c\/h3\u003e\n\n\u003cp\u003e通常作为 2'-O-MOE\/DNA 嵌合体，激活 RNase H 介导的目标 RNA 裂解。\u003csup\u003e[1]\u003c\/sup\u003e\u003c\/p\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"rte-list-card\"\u003e\n\n\u003ch3 class=\"rte-dashed-list-title\"\u003e\n\n\u003csvg class=\"rte-list-icon\" viewbox=\"0 0 640 640\"\u003e\n        \u003cpath fill=\"currentColor\" d=\"M406.6 73.4C394.1 60.9 373.8 60.9 361.3 73.4C348.8 85.9 348.8 106.2 361.3 118.7L370.7 128L92.1 406.6C74.1 424.6 64 449 64 474.5L64 480C64 533 107 576 160 576L165.5 576C191 576 215.4 565.9 233.4 547.9L512 269.3L521.4 278.7C533.9 291.2 554.2 291.2 566.7 278.7C579.2 266.2 579.2 245.9 566.7 233.4L406.7 73.4zM269.3 320L416 173.3L466.7 224L370.7 320L269.2 320z\"\u003e \u003c\/path\u003e\n      \u003c\/svg\u003e 空间位阻型 ASO\u003c\/h3\u003e\n\n\u003cp\u003e物理性地阻碍特定 RNA 分子的接触，而非导致其降解，例如剪接转换 ASO。\u003csup\u003e[1]\u003c\/sup\u003e\u003c\/p\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"rte-list-card\"\u003e\n\n\u003ch3 class=\"rte-dashed-list-title\"\u003e\n\n\u003csvg class=\"rte-list-icon\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewbox=\"0 0 640 640\"\u003e\u003cpath d=\"M480 64C497.7 64 512 78.3 512 96C512 153.8 487.6 200.8 454.6 240.5C430.5 269.4 400.8 295.6 371 320C400.8 344.5 430.5 370.6 454.6 399.5C487.6 439.1 512 486.2 512 544C512 561.7 497.7 576 480 576C462.3 576 448 561.7 448 544L192 544C192 561.7 177.7 576 160 576C142.3 576 128 561.7 128 544C128 486.2 152.4 439.2 185.4 399.5C209.5 370.6 239.2 344.5 269 320C239.2 295.5 209.5 269.4 185.4 240.5C152.4 200.8 128 153.8 128 96C128 78.3 142.3 64 160 64C177.7 64 192 78.3 192 96L448 96C448 78.3 462.3 64 480 64zM411.5 448L228.6 448C220.4 458.5 213.5 469.1 208 480L432.2 480C426.6 469.1 419.7 458.5 411.6 448zM366 400C351.7 387 336.2 374.2 320 361C303.8 374.1 288.3 387 274 400L366 400zM228.5 192L411.4 192C419.6 181.5 426.5 170.9 432 160L207.9 160C213.5 170.9 220.4 181.5 228.5 192zM274 240C288.3 253 303.8 265.8 320 279C336.2 265.9 351.7 253 366 240L274 240z\"\u003e\u003c\/path\u003e\u003c\/svg\u003e 酶或结构 RNA 抑制\u003c\/h3\u003e\n\n\u003cp\u003e通过结合干扰抑制特定的 RNA 或酶（例如端粒酶）。\u003csup\u003e[2]\u003c\/sup\u003e\u003c\/p\u003e\n\n\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003e特点与优势\u003c\/h3\u003e\n\u003cdiv class=\"rte-list-feature\"\u003e固相 DNA 合成中高效的偶联效率和重复性\u003csvg width=\"10\" height=\"10\" viewbox=\"0 0 300 300\"\u003e\n    \u003cpolygon points=\"50,250 250,50 250,250\"\u003e\u003c\/polygon\u003e\n  \u003c\/svg\u003e\n\n\u003c\/div\u003e\n\u003cdiv class=\"rte-list-feature\"\u003e高纯度（≥ 98%）和严格控制的杂质谱\u003csvg width=\"10\" height=\"10\" viewbox=\"0 0 300 300\"\u003e\n    \u003cpolygon points=\"50,250 250,50 250,250\"\u003e\u003c\/polygon\u003e\n  \u003c\/svg\u003e\n\n\u003c\/div\u003e\n\u003cdiv class=\"rte-list-feature\"\u003e与标准 DNA\/RNA 亚磷酰胺兼容\u003csvg width=\"10\" height=\"10\" viewbox=\"0 0 300 300\"\u003e\n    \u003cpolygon points=\"50,250 250,50 250,250\"\u003e\u003c\/polygon\u003e\n  \u003c\/svg\u003e\n\n\u003c\/div\u003e\n\u003cdiv class=\"rte-list-feature\"\u003e简化纯化：无需针对 RNase 降解采取特殊预防措施\u003csvg width=\"10\" height=\"10\" viewbox=\"0 0 300 300\"\u003e\n    \u003cpolygon points=\"50,250 250,50 250,250\"\u003e\u003c\/polygon\u003e\n  \u003c\/svg\u003e\n\n\u003c\/div\u003e\n\u003cdiv class=\"rte-list-feature\" style=\"margin-bottom: 20px;\"\u003e可根据要求提供快速脱保护变体\u003csvg width=\"10\" height=\"10\" viewbox=\"0 0 300 300\"\u003e\n    \u003cpolygon points=\"50,250 250,50 250,250\"\u003e\u003c\/polygon\u003e\n  \u003c\/svg\u003e\n\n\u003c\/div\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003e其他说明\u003c\/h3\u003e\n\u003cul style=\"margin-inline-start: 0; padding-inline-start: 1.35em;\"\u003e\n\n\u003cli\u003e\n\n\u003cstrong\u003e储存：\u003c\/strong\u003e在 -20 °C 干燥、惰性气氛中储存。\u003c\/li\u003e\n\n\u003cli\u003e\n\n\u003cstrong\u003e偶联：\u003c\/strong\u003e建议偶联时间为 6 分钟\u003c\/li\u003e\n\n\u003cli\u003e\n\n\u003cstrong\u003e推荐脱保护：\u003c\/strong\u003e在浓氨水中 55 °C 下 8 小时，或在 AMA（氨水\/甲胺，1:1 v\/v）中 65 °C 下 10 分钟\u003c\/li\u003e\n\n\u003cli\u003e\n\n\u003cstrong\u003e兼容性：\u003c\/strong\u003e可与修饰的亚磷酰胺（例如 \u003ca href=\"https:\/\/shop.hongene.com\/collections\/2-o-me-phosphoramidites\"\u003e2′-OMe\u003c\/a\u003e、\u003ca href=\"https:\/\/shop.hongene.com\/collections\/2-o-moe-phosphoramidites\"\u003e2′-MOE\u003c\/a\u003e、\u003ca href=\"https:\/\/shop.hongene.com\/collections\/2-o-4-c-locked-na-phosphoramidites\"\u003eLocked-NA\u003c\/a\u003e）一起使用，以合成嵌合寡核苷酸。\u003c\/li\u003e\n\n\n\u003c\/ul\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003e参考文献\u003c\/h3\u003e\n\u003cp\u003e\u003ccite\u003e[1] Hill, Alyssa C., and Jonathan Hall. \"The MOE modification of RNA: Origins and widescale impact on the oligonucleotide therapeutics field.\" Helvetica Chimica Acta 106, no. 3 (2023): e202200169.\u003c\/cite\u003e\u003cbr\u003e\u003ccite\u003e[2] 2.Elayadi, Anissa N., Andrea Demieville, Edward V. Wancewicz, Brett P. Monia, and David R. Corey. \"Inhibition of telomerase by 2′-O-(2-methoxyethyl) RNA oligomers: effect of length, phosphorothioate substitution and time inside cells.\" Nucleic Acids Research 29, no. 8 (2001): 1683-1689.\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"N（普通） \/ 0.25克\/30毫升旋盖瓶-28","offer_id":44541323968712,"sku":"PR3-007-025A","price":15.0,"currency_code":"USD","in_stock":true},{"title":"N（普通） \/ 0.5克\/30毫升旋口瓶-28","offer_id":44541324001480,"sku":"PR3-007-05A","price":20.0,"currency_code":"USD","in_stock":true},{"title":"N（普通） \/ 1克\/30毫升旋口瓶-28","offer_id":44541324034248,"sku":"PR3-007-1A","price":23.0,"currency_code":"USD","in_stock":true},{"title":"N（普通） \/ 5克\/240毫升螺旋盖瓶","offer_id":44541324067016,"sku":"PR3-007-5B","price":68.0,"currency_code":"USD","in_stock":true},{"title":"N（普通） \/ 10克\/240毫升旋口瓶","offer_id":44541324099784,"sku":"PR3-007-10B","price":115.0,"currency_code":"USD","in_stock":true},{"title":"N（普通） \/ 25克\/250毫升高密度聚乙烯瓶","offer_id":44541324132552,"sku":"PR3-007-25C","price":295.0,"currency_code":"USD","in_stock":true},{"title":"N（普通） \/ 100克\/250毫升高密度聚乙烯瓶","offer_id":44541324165320,"sku":"PR3-007-100C","price":975.0,"currency_code":"USD","in_stock":true},{"title":"N（普通） \/ 200克\/500毫升HDPE瓶","offer_id":44541324198088,"sku":"PR3-007-200D","price":1800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/PR3-007_240f25f3-4b54-4b4e-9e0b-f0178b24c195.jpg?v=1748589700","url":"https:\/\/shop.hongene.com\/zh\/products\/5-me-dmt-2-o-moe-c-bz-ce-phosphoramidite","provider":"Hongene Online Store","version":"1.0","type":"link"}