{"product_id":"dmt-2-o-tbdms-c-ac-ce-phosphoramidite","title":"DMT-2'-O-TBDMS-C(Ac)-CE 亚磷酰胺","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n    margin-top: 20px;\n}\n.rte-des-lists {\n    display: flex;\n    flex-wrap: wrap;\n    gap: 25px;\n    padding: 0 10px;\n    margin-bottom: 25px;\n}\n.rte-list-card {\n    flex: 1;\n    background: rgba(239, 238, 236, 0.3);\n    border-radius: 10px;\n    padding: 20px;\n    box-shadow: 0px 3px 10px rgba(22, 47, 101, 0.2);\n    transition: transform 0.3s;\n    min-width: 290px;\n    position: relative;\n}\n.rte-list-card:hover {\n    transform: translateY(-5px);\n}\n.rte-list-icon {\n    width: 25px;\n    height: 25px;\n    margin: 0px 5px -5px 0px;\n    fill: currentColor;\n}\n.rte-list-card h3 {\n    font-size: 1.2em;\n    padding-bottom: 8px;\n    margin-bottom: 8px;\n    color: #3361ac;\n}\n.rte-dashed-list-title {\n    border-bottom: dashed rgba(193, 212, 231);\n}\n.rte-list-feature {\n    border-left: solid 3px #3361ac;\n    padding: 3px 0 3px 10px;\n    margin: 0 5px 5px 0;\n}\n.rte-list-feature svg {\n    width: 10px;\n    height: 10px;\n    margin-bottom: -5px;\n    fill: #3361ac;\n}\n.cte-cite cite {\n    display: block;\n    margin-bottom: 8px;\n}\n\u003c\/style\u003e\n\u003cp\u003eDMT-2'-O-TBDMS-C(Ac)-CE-Phosphoramidite 是一种受保护的核糖核苷酸构建模块，用于固相 RNA 寡核苷酸合成。它包含一个用于 5'-羟基保护的 5'-二甲氧基三苯甲基 (DMT) 保护基团、一个用于 2'-羟基保护的 \u003cstrong\u003e2'-O-叔丁基二甲基硅烷基 (TBDMS)\u003c\/strong\u003e 保护基团、一个磷酸酯上的氰乙基 (CE) 保护基团以及一个胞嘧啶上的乙酰基 (Ac) 碱基保护基团。\u003c\/p\u003e\n\u003cp\u003eTBDMS 在酸脱三苯甲基化和氧化步骤中，为\u003cstrong\u003e核糖核苷酸的 2'-羟基保护提供了出色的稳定性\u003c\/strong\u003e。\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTBDMS 脱保护可以使用氟化物试剂\u003c\/strong\u003e（例如，四丁基氟化铵 (TBAF) 和三乙胺三氢氟化物 (TEA·3HF)）实现，这些试剂可以在不破坏 RNA 主链的情况下裂解硅烷基。\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003e应用\u003c\/h3\u003e\n\u003cdiv class=\"rte-des-lists\"\u003e\n\n\u003cdiv class=\"rte-list-card\"\u003e\n\n\u003ch3 class=\"rte-dashed-list-title\"\u003e\n\n\u003csvg viewbox=\"0 0 640 640\" class=\"rte-list-icon\"\u003e\n        \u003cpath d=\"M406.6 73.4C394.1 60.9 373.8 60.9 361.3 73.4C348.8 85.9 348.8 106.2 361.3 118.7L370.7 128L92.1 406.6C74.1 424.6 64 449 64 474.5L64 480C64 533 107 576 160 576L165.5 576C191 576 215.4 565.9 233.4 547.9L512 269.3L521.4 278.7C533.9 291.2 554.2 291.2 566.7 278.7C579.2 266.2 579.2 245.9 566.7 233.4L406.7 73.4zM269.3 320L416 173.3L466.7 224L370.7 320L269.2 320z\" fill=\"currentColor\"\u003e \u003c\/path\u003e\n      \u003c\/svg\u003e 合成 RNA 寡核苷酸\u003c\/h3\u003e\n\n\u003cp\u003e用于制备生化分析和结合研究中使用的功能性 RNA 序列的单体，\u003csup\u003e[1]\u003c\/sup\u003e 例如 RNA 适体和核酶。\u003c\/p\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"rte-list-card\"\u003e\n\n\u003ch3 class=\"rte-dashed-list-title\"\u003e\n\n\u003csvg viewbox=\"0 0 640 640\" class=\"rte-list-icon\"\u003e\n        \u003cpath d=\"M406.6 73.4C394.1 60.9 373.8 60.9 361.3 73.4C348.8 85.9 348.8 106.2 361.3 118.7L370.7 128L92.1 406.6C74.1 424.6 64 449 64 474.5L64 480C64 533 107 576 160 576L165.5 576C191 576 215.4 565.9 233.4 547.9L512 269.3L521.4 278.7C533.9 291.2 554.2 291.2 566.7 278.7C579.2 266.2 579.2 245.9 566.7 233.4L406.7 73.4zM269.3 320L416 173.3L466.7 224L370.7 320L269.2 320z\" fill=\"currentColor\"\u003e \u003c\/path\u003e\n      \u003c\/svg\u003e 混合骨架设计\u003c\/h3\u003e\n\n\u003cp\u003e可实现 RNA-DNA 嵌合体的合成，例如基于嵌合 RNA\/DNA 寡核苷酸的基因治疗。\u003csup\u003e[2][3]\u003c\/sup\u003e\u003c\/p\u003e\n\n\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003e特点和优势\u003c\/h3\u003e\n\u003cdiv class=\"rte-list-feature\"\u003e在酸性脱三苯甲基化和氧化步骤中稳定\u003csvg viewbox=\"0 0 300 300\" height=\"10\" width=\"10\"\u003e\n    \u003cpolygon points=\"50,250 250,50 250,250\"\u003e\u003c\/polygon\u003e\n  \u003c\/svg\u003e\n\n\u003c\/div\u003e\n\u003cdiv class=\"rte-list-feature\"\u003e与其他核糖核苷酸亚磷酰胺兼容\u003csvg viewbox=\"0 0 300 300\" height=\"10\" width=\"10\"\u003e\n    \u003cpolygon points=\"50,250 250,50 250,250\"\u003e\u003c\/polygon\u003e\n  \u003c\/svg\u003e\n\n\u003c\/div\u003e\n\u003cdiv class=\"rte-list-feature\"\u003e在标准 RNA 合成条件下具有高偶联效率\u003csvg viewbox=\"0 0 300 300\" height=\"10\" width=\"10\"\u003e\n    \u003cpolygon points=\"50,250 250,50 250,250\"\u003e\u003c\/polygon\u003e\n  \u003c\/svg\u003e\n\n\u003c\/div\u003e\n\u003cdiv class=\"rte-list-feature\"\u003e灵活的脱保护方案，即与 TEA·3HF 或其他用于去除 TBDMS 的氟化物源兼容\u003csvg viewbox=\"0 0 300 300\" height=\"10\" width=\"10\"\u003e\n    \u003cpolygon points=\"50,250 250,50 250,250\"\u003e\u003c\/polygon\u003e\n  \u003c\/svg\u003e\n\n\u003c\/div\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003e其他注意事项\u003c\/h3\u003e\n\u003cul style=\"margin-inline-start: 0; padding-inline-start: 1.35em;\"\u003e\n\n\u003cli\u003e\n\n\u003cstrong\u003e储存：\u003c\/strong\u003e在干燥、惰性气氛下于 -20 °C 储存。\u003c\/li\u003e\n\n\u003cli\u003e\n\n\u003cstrong\u003e偶联：\u003c\/strong\u003e建议偶联时间为 12 分钟\u003c\/li\u003e\n\n\u003cli\u003e\n\n\u003cstrong\u003e兼容性：\u003c\/strong\u003e可与修饰的亚磷酰胺（例如 \u003ca href=\"https:\/\/shop.hongene.com\/collections\/2-o-me-phosphoramidites\"\u003e2′-OMe\u003c\/a\u003e、\u003ca href=\"https:\/\/shop.hongene.com\/collections\/2-o-moe-phosphoramidites\"\u003e2′-MOE\u003c\/a\u003e、\u003ca href=\"https:\/\/shop.hongene.com\/collections\/2-o-4-c-locked-na-phosphoramidites\"\u003eLocked-NA\u003c\/a\u003e）一起使用，以合成嵌合寡核苷酸。\u003c\/li\u003e\n\n\n\u003c\/ul\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003e参考文献\u003c\/h3\u003e\n\u003cdiv class=\"cte-cite\"\u003e\n\n\u003ccite\u003e[1] Sproat, Brian S. \"RNA synthesis using 2′-O-(tert-butyldimethylsilyl) protection.\" In Oligonucleotide Synthesis, pp. 17-31. Totowa, NJ: Humana Press, 2005.\u003c\/cite\u003e \u003ccite\u003e[2] Lai, Li-Wen, and Yeong-Hau H. Lien. \"Chimeric RNA\/DNA oligonucleotide-based gene therapy.\" Kidney International 61, no. 1 (2002): S47-S51. \u003c\/cite\u003e \u003ccite\u003e[3] Li, Nan-Sheng, John K. Frederiksen, and Joseph A. Piccirilli. \"Synthesis, properties, and applications of oligonucleotides containing an RNA dinucleotide phosphorothiolate linkage.\" Accounts of chemical research 44, no. 12 (2011): 1257-1269.\u003c\/cite\u003e\n\n\u003c\/div\u003e","brand":"Hongene","offers":[{"title":"正常 (Normal) \/ 0.25克\/30毫升旋盖瓶-28","offer_id":44541337403592,"sku":"PR3-008-025A","price":20.0,"currency_code":"USD","in_stock":true},{"title":"正常 (Normal) \/ 0.5克\/30毫升螺口瓶-28","offer_id":44541337436360,"sku":"PR3-008-05A","price":27.0,"currency_code":"USD","in_stock":true},{"title":"正常 (Normal) \/ 1克\/30毫升旋盖瓶-28","offer_id":44541337469128,"sku":"PR3-008-1A","price":30.0,"currency_code":"USD","in_stock":true},{"title":"正常 (Normal) \/ 5克\/240毫升螺旋盖瓶","offer_id":44541337501896,"sku":"PR3-008-5B","price":85.0,"currency_code":"USD","in_stock":true},{"title":"正常 (Normal) \/ 10克\/240毫升螺口瓶","offer_id":44541337534664,"sku":"PR3-008-10B","price":150.0,"currency_code":"USD","in_stock":true},{"title":"正常 (Normal) \/ 25克\/250毫升高密度聚乙烯瓶","offer_id":44541337567432,"sku":"PR3-008-25C","price":400.0,"currency_code":"USD","in_stock":true},{"title":"正常 (Normal) \/ 100克\/250毫升高密度聚乙烯瓶","offer_id":44541337600200,"sku":"PR3-008-100C","price":1350.0,"currency_code":"USD","in_stock":true},{"title":"正常 (Normal) \/ 200克\/500毫升高密度聚乙烯瓶","offer_id":44541337632968,"sku":"PR3-008-200D","price":2600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/PR3-008_d917ea90-a8c7-4b61-8771-b244ac54c0c3.jpg?v=1748589632","url":"https:\/\/shop.hongene.com\/zh\/products\/dmt-2-o-tbdms-c-ac-ce-phosphoramidite","provider":"Hongene Online Store","version":"1.0","type":"link"}