{"product_id":"dnase-i-rnase-free","title":"DNase I (RNase-free)","description":"\u003cstyle\u003e\n.rte-hr {\n    background: linear-gradient(to right, white, #162F65);\n    border: 0;\n    height: 1px;\n}\n.rte-companion-products {\n    display: flex;\n    gap: 10px;\n    margin-bottom: 25px;\n}\n.rte-companion-link {\n    padding: 8px;\n    margin: 5px;\n    max-width: 450px;\n    border-radius: 8px;\n    box-shadow: 0 0 5px #162f6580;\n    text-decoration: none !important;\n    transition: transform 0.3s;\n}\n.rte-companion-link:hover {\n    transform: translateY(-5px);\n}\n.rte-companion-product {\n    display: flex;\n    gap: 10px;\n}\n.companion-product-img {\n    width: 46%;\n    min-width: 150px;\n    max-width: 220px;\n}\n.companion-product-content {\n    width: 50%;\n}\n.rte-product-catalog, .rte-product-volumn {\n    font-size: 14px;\n    display: block;\n    margin-top: 5px;\n    color: #162f65;\n}\n.rte-product-title {\n    color: #3361ac;\n    font-weight: 700;\n    display: block;\n    margin-bottom: 5px;\n}\n.rte-points-operation li {\n    margin-bottom: 1em;\n}\n.rte-points-operation span {\n    display: block;\n    margin-bottom: 0.5em;\n}\n\n@media (max-width: 768px) {\n.rte-companion-products {\n    flex-direction: column;\n}\n.rte-companion-link {\n    width: 95%;\n}\n}\n\u003c\/style\u003e\n\u003ch3\u003e产品信息\u003c\/h3\u003e\n\u003cp\u003eDNase I（无 RNase）是一种内切核酸酶，可消化单链和双链 DNA。它能非特异性地切割 DNA，释放出具有 5' 磷酸化和 3' 羟基末端的二核苷酸、三核苷酸及寡核苷酸产物。\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e来源\u003c\/strong\u003e：重组大肠杆菌\u003cbr\u003e\u003cstrong\u003e浓度\u003c\/strong\u003e：1U\/μL\u003cbr\u003e\u003cstrong\u003e单位定义\u003c\/strong\u003e：一个单位定义为在 37°C 下 10 分钟内完全降解 1μg λDNA 所需的酶量。\u003cbr\u003e\u003cstrong\u003e储存缓冲液\u003c\/strong\u003e：50mM Tris-HCl（pH7.5，25℃），10mM CaCl\u003csub\u003e2\u003c\/sub\u003e，50% 甘油\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e配套产品：\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"rte-companion-products\"\u003e\u003ca class=\"rte-companion-link\" href=\"https:\/\/shop.hongene.com\/products\/10x-dnase-i-reaction-buffer\" title=\"跳转至 ON-077\"\u003e\n\u003cdiv class=\"rte-companion-product\"\u003e\n\u003cdiv class=\"companion-product-img\"\u003e\u003csvg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 600 600\" style=\"enable-background: new 0 0 600 600; border-radius: 8px; border: solid 1px #162f65;\" xml:space=\"preserve\"\u003e\n        \u003cstyle type=\"text\/css\"\u003e\n\t.st0{fill:#BFBFBF;}\n\t.st1{fill:none;stroke:#bfbfbf;stroke-width:21.1099;stroke-miterlimit:10;}\n\t.st2{fill:none;stroke:#bfbfbf;stroke-width:24.3757;stroke-miterlimit:10;}\n\u003c\/style\u003e\n        \u003cg\u003e\n          \u003cpath class=\"st0\" d=\"M282.4,390.4c-9.79,0-17.75,7.96-17.75,17.75c0,9.79,7.96,17.76,17.75,17.76c9.8,0,17.76-7.97,17.76-17.76\n\t\tC300.17,398.37,292.21,390.4,282.4,390.4z M282.4,418.81c-3.8,0-7.32-2.03-9.22-5.33c-1.9-3.3-1.9-7.36,0-10.65\n\t\tc1.91-3.3,5.43-5.33,9.23-5.33c5.89,0.01,10.65,4.78,10.65,10.66C293.07,414.05,288.3,418.81,282.4,418.81z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st0\" d=\"M300,446.01c-6.59,0-11.94,5.36-11.94,11.94c0,6.59,5.36,11.95,11.94,11.95c6.59,0,11.95-5.36,11.95-11.95\n\t\tC311.94,451.37,306.59,446.01,300,446.01z M300,465.12c-2.56,0-4.92-1.37-6.2-3.59c-1.28-2.22-1.28-4.95,0-7.17\n\t\tc1.29-2.22,3.65-3.59,6.21-3.59c3.96,0.01,7.17,3.22,7.17,7.17C307.17,461.91,303.96,465.12,300,465.12z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st0\" d=\"M311.34,314.76c-14.11,0-25.58,11.47-25.58,25.58c0,14.11,11.47,25.6,25.58,25.6c14.12,0,25.6-11.49,25.6-25.6\n\t\tC336.94,326.23,325.47,314.76,311.34,314.76z M311.34,355.7c-5.48,0-10.55-2.93-13.29-7.68c-2.74-4.75-2.74-10.6,0-15.35\n\t\tc2.75-4.75,7.82-7.68,13.3-7.68c8.49,0.01,15.35,6.89,15.35,15.37C326.71,348.83,319.83,355.7,311.34,355.7z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st1\" d=\"M357.17,132.88v312.25c0,0.3,0,0.58-0.02,0.88c-0.46,31.17-25.88,56.29-57.16,56.29s-56.7-25.12-57.16-56.29\n\t\tc-0.02-0.3-0.02-0.58-0.02-0.88V132.88H357.17z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st0\" d=\"M299.99,445.13c0,0.3,0,0.58,0.02,0.88h-0.02V445.13z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st0\" d=\"M300.01,445.13v0.88h-0.02C300.01,445.71,300.01,445.43,300.01,445.13z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st2\" d=\"M366.41,132.88H233.59c-9.68,0-17.59-7.92-17.59-17.59v0c0-9.68,7.92-17.59,17.59-17.59h132.82\n\t\tc9.68,0,17.59,7.92,17.59,17.59v0C384,124.96,376.08,132.88,366.41,132.88z\"\u003e\u003c\/path\u003e\n          \u003cpath class=\"st1\" d=\"M242.84,257.19c0,0,79.13,35.97,114.33-40.66\"\u003e\u003c\/path\u003e\n        \u003c\/g\u003e\n      \u003c\/svg\u003e\u003c\/div\u003e\n\u003cdiv class=\"companion-product-content\"\u003e\n\u003cdiv class=\"rte-product-catalog\"\u003e目录号：ON-077\u003c\/div\u003e\n\u003cdiv class=\"rte-product-title\"\u003e10X DNase I 反应缓冲液\u003c\/div\u003e\n\u003cdiv class=\"rte-product-volumn\"\u003e\n\u003cdiv style=\"font-size: 12px; margin-bottom: 5px;\"\u003e内含成分：\u003c\/div\u003e\n\u003cdiv\u003e- 100mM Tris-HCl pH7.6，25℃\u003cbr\u003e- 25mM MgCl\u003csub\u003e2\u003c\/sub\u003e\u003cbr\u003e- 5mM CaCl\u003csub\u003e2\u003c\/sub\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003e操作要点\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eIVT 反应后的 DNase I 处理：\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e直接将 DNase I（每 μg DNA 模板加入 1 单位 DNase I）加入 IVT 反应液中，混匀后于 37°C 孵育 30 分钟。\u003c\/li\u003e\n\u003cli\u003e加入 0.5M EDTA 至终浓度高于二价阳离子浓度 1 mM 以上。\u003c\/li\u003e\n\u003cli\u003e75°C 加热 10 分钟使酶热失活。\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003e安全信息\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003e储存条件\u003c\/strong\u003e：-20℃\u003cbr\u003e\u003cstrong\u003e质量保证\u003c\/strong\u003e：无 RNase 活性污染。\u003cbr\u003e\u003cstrong\u003e物理纯度\u003c\/strong\u003e：SDS-PAGE 检测 \u0026gt;95%。\u003c\/p\u003e\n\u003chr class=\"rte-hr\"\u003e\n\u003ch3\u003e参考文献\u003c\/h3\u003e\n\u003cp\u003e\u003ccite\u003e1. A F Worrall，B A Connolly, J，Biol，Chem. 265,21889–21896 (1990).\u003c\/cite\u003e\u003cbr\u003e\u003ccite\u003e2. Ching-Ying Chen，Shao-Chun Lu，et al. Gene. 206, 181-184(1998).\u003c\/cite\u003e\u003c\/p\u003e","brand":"Hongene","offers":[{"title":"M (Molecular Biology) \/ Non-GMP \/ 1kU \/ centrifuge tube","offer_id":44579127066824,"sku":"ON-109-KU001-P","price":25.0,"currency_code":"USD","in_stock":true},{"title":"M (Molecular Biology) \/ GMP \/ 1kU \/ centrifuge tube","offer_id":44579127099592,"sku":"ON-109-G","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0682\/8228\/6280\/files\/Biochemical-P1_bdbb30ba-9665-4b52-a90c-cd69b496f14f.jpg?v=1748588694","url":"https:\/\/shop.hongene.com\/zh\/products\/dnase-i-rnase-free","provider":"Hongene Online Store","version":"1.0","type":"link"}