2'-O-4'-C-锁核苷G(dmf)亚磷酰胺

N2-二甲基甲脒-5'-O-(4, 4'-二甲氧基三苯甲基)-2'-O-4'-C-锁定-鸟苷-3'-氰乙基亚磷酰胺

2'-O-4'-C-锁核苷G(dmf)亚磷酰胺

N2-二甲基甲脒-5'-O-(4, 4'-二甲氧基三苯甲基)-2'-O-4'-C-锁定-鸟苷-3'-氰乙基亚磷酰胺
年级
金额/套餐
SKU： PR2-005-025A  |  suitable for  美人鱼手工坊
收藏夹:  2'-O-4'-C锁定核酸磷酰胺 亚磷酰胺 合成砌块 寡核苷酸合成
单价:
$34.00
价格
$34.00
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    详情

    2’-O-4’-C-Locked-G(dmf) 亚磷酰胺是一种锁定核酸（LNA）亚磷酰胺单体，用于固相寡核苷酸合成。

    它含有一个双环核糖，其中亚甲基桥连接2'-氧（O）和4'-碳（C），将糖锁定在C3'-内型构象。它还含有一个带有二甲基甲酰胺基（dmf）碱基保护的鸟嘌呤（G）、一个用于5'-羟基保护的5'-二甲氧基三苯甲基（DMT）基团和一个亚磷酸酯上的氰乙基（CE）保护基团。

    LNA修饰的寡核苷酸显示出增强的结合强度、特异性和核酸酶抗性。

    应用

    检测与诊断

    LNA修饰的寡核苷酸提供卓越的双链稳定性，可实现高灵敏度检测，例如杂交探针和qPCR引物。[1][2]

    基因分型与序列区分

    LNA赋予的尖锐错配识别能力增强了SNP基因分型和等位基因特异性PCR。[3][4]

    治疗性寡核苷酸研究

    LNA修饰用于增加ASO的结合亲和力和核酸酶抗性。[5][6]

    特点

    高纯度：生产纯度≥98%，杂质规格严格控制。
    兼容性：与标准亚磷酰胺化学和大多数商用合成仪兼容。
    卓越的偶联效率和产率。

    其他注意事项

    • 稀释剂：无水乙腈。
    • 储存：在-20°C的干燥惰性气氛下储存。
    • 偶联：建议偶联时间为6分钟。

    参考文献

    [1] Várallyay, Eva, József Burgyán, and Zoltán Havelda. "MicroRNA detection by northern blotting using locked nucleic acid probes." Nature protocols 3, no. 2 (2008): 190-196. [2] Ballantyne, K. N., R. A. H. Van Oorschot, and R. J. Mitchell. "Locked nucleic acids in PCR primers increase sensitivity and performance." Genomics 91, no. 3 (2008): 301-305. [3] Mouritzen, Peter, Alex Toftgaard Nielsen, Henrik M. Pfundheller, Yoanna Choleva, Lars Kongsbak, and Søren Møller. "Single nucleotide polymorphism genotyping using locked nucleic acid (LNA™)." Expert review of molecular diagnostics 3, no. 1 (2003): 27-38. [4] Latorra, David, Krista Campbell, Andreas Wolter, and J. Michael Hurley. "Enhanced allele‐specific PCR discrimination in SNP genotyping using 3′ locked nucleic acid (LNA) primers." Human mutation 22, no. 1 (2003): 79-85. [5]Kurreck, Jens, Eliza Wyszko, Clemens Gillen, and Volker A. Erdmann. "Design of antisense oligonucleotides stabilized by locked nucleic acids." Nucleic acids research 30, no. 9 (2002): 1911-1918. [6] Wahlestedt, Claes, Peter Salmi, Liam Good, Johanna Kela, Thomas Johnsson, Tomas Hökfelt, Christian Broberger et al. "Potent and nontoxic antisense oligonucleotides containing locked nucleic acids." Proceedings of the National Academy of Sciences 97, no. 10 (2000): 5633-5638.

    规格
    • 目录号
      PR2-005
    • CAS号
      709641-79-2
    • SMILES
      O=C1C(N=CN2[C@H]3[C@H](OC4)[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@]4(COC(C5=CC=CC=C5)(C6=CC=C(OC)C=C6)C7=CC=C(OC)C=C7)O3)=C2N=C(/N=C/N(C)C)N1
    • 分子式
      C44H53N8O8P
    • 分子量
      852.93
    • 外观
      白色、类白色至微黄色粉末，无可见异物
    • 纯度
      HPLC ≥98.0%
    • 储存条件
      -20℃
    • 水分含量
      K.F.≤0.50% (w/w)

    文档
    COA 联系我们
    规格书 下载

    2’-O-4’-C-Locked-G(dmf) 亚磷酰胺是一种锁定核酸（LNA）亚磷酰胺单体，用于固相寡核苷酸合成。

    它含有一个双环核糖，其中亚甲基桥连接2'-氧（O）和4'-碳（C），将糖锁定在C3'-内型构象。它还含有一个带有二甲基甲酰胺基（dmf）碱基保护的鸟嘌呤（G）、一个用于5'-羟基保护的5'-二甲氧基三苯甲基（DMT）基团和一个亚磷酸酯上的氰乙基（CE）保护基团。

    LNA修饰的寡核苷酸显示出增强的结合强度、特异性和核酸酶抗性。

    应用

    检测与诊断

    LNA修饰的寡核苷酸提供卓越的双链稳定性，可实现高灵敏度检测，例如杂交探针和qPCR引物。[1][2]

    基因分型与序列区分

    LNA赋予的尖锐错配识别能力增强了SNP基因分型和等位基因特异性PCR。[3][4]

    治疗性寡核苷酸研究

    LNA修饰用于增加ASO的结合亲和力和核酸酶抗性。[5][6]

    特点

    高纯度：生产纯度≥98%，杂质规格严格控制。
    兼容性：与标准亚磷酰胺化学和大多数商用合成仪兼容。
    卓越的偶联效率和产率。

    其他注意事项

    • 稀释剂：无水乙腈。
    • 储存：在-20°C的干燥惰性气氛下储存。
    • 偶联：建议偶联时间为6分钟。

    参考文献

    [1] Várallyay, Eva, József Burgyán, and Zoltán Havelda. "MicroRNA detection by northern blotting using locked nucleic acid probes." Nature protocols 3, no. 2 (2008): 190-196. [2] Ballantyne, K. N., R. A. H. Van Oorschot, and R. J. Mitchell. "Locked nucleic acids in PCR primers increase sensitivity and performance." Genomics 91, no. 3 (2008): 301-305. [3] Mouritzen, Peter, Alex Toftgaard Nielsen, Henrik M. Pfundheller, Yoanna Choleva, Lars Kongsbak, and Søren Møller. "Single nucleotide polymorphism genotyping using locked nucleic acid (LNA™)." Expert review of molecular diagnostics 3, no. 1 (2003): 27-38. [4] Latorra, David, Krista Campbell, Andreas Wolter, and J. Michael Hurley. "Enhanced allele‐specific PCR discrimination in SNP genotyping using 3′ locked nucleic acid (LNA) primers." Human mutation 22, no. 1 (2003): 79-85. [5]Kurreck, Jens, Eliza Wyszko, Clemens Gillen, and Volker A. Erdmann. "Design of antisense oligonucleotides stabilized by locked nucleic acids." Nucleic acids research 30, no. 9 (2002): 1911-1918. [6] Wahlestedt, Claes, Peter Salmi, Liam Good, Johanna Kela, Thomas Johnsson, Tomas Hökfelt, Christian Broberger et al. "Potent and nontoxic antisense oligonucleotides containing locked nucleic acids." Proceedings of the National Academy of Sciences 97, no. 10 (2000): 5633-5638.

    Why choose Hongene?

    Trusted Partner in Nucleic Acid

    Enjoy enterprise‑grade quality even with small batch orders.

    Integrated Supply & Commercial Scale

    With nearly 30 years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

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    Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

    Rigorous Quality

    We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

    Manufacturing Scalability

    Hongene operates a 1.67-million-square-foot oligonucleotide manufacturing facility featuring advanced equipment, including multiple OligoPilot™ and OligoProcess™ synthesizers ranging from 10 to 1,800 mmol. Its 48 flexible production lines enable seamless, one-stop scale-up of API production from gram-level quantities to metric tons, achieving purity of ≥98% while supporting compliance with NMPA, FDA, and EMA regulatory requirements.

    Global Business Network

    Our products and services reach over 40 countries and regions, supporting around 3,000 clients worldwide.

    Global Business Distribution
    40+
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    ≈3000
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    54000L+
    Annual NTP Production
    58t+
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