DNase I (RNase-free)
DNase I (RNase-free) - M (Molecular Biology) / Non-GMP / 1kU / centrifuge tube 缺货,一旦补货将尽快发货。
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Details
Details
产品信息
DNase I(无 RNase)是一种内切核酸酶,可消化单链和双链 DNA。它能非特异性地切割 DNA,释放出具有 5' 磷酸化和 3' 羟基末端的二核苷酸、三核苷酸及寡核苷酸产物。
来源:重组大肠杆菌
浓度:1U/μL
单位定义:一个单位定义为在 37°C 下 10 分钟内完全降解 1μg λDNA 所需的酶量。
储存缓冲液:50mM Tris-HCl(pH7.5,25℃),10mM CaCl2,50% 甘油
配套产品:
操作要点
IVT 反应后的 DNase I 处理:
- 直接将 DNase I(每 μg DNA 模板加入 1 单位 DNase I)加入 IVT 反应液中,混匀后于 37°C 孵育 30 分钟。
- 加入 0.5M EDTA 至终浓度高于二价阳离子浓度 1 mM 以上。
- 75°C 加热 10 分钟使酶热失活。
安全信息
储存条件:-20℃
质量保证:无 RNase 活性污染。
物理纯度:SDS-PAGE 检测 >95%。
参考文献
1. A F Worrall,B A Connolly, J,Biol,Chem. 265,21889–21896 (1990).
2. Ching-Ying Chen,Shao-Chun Lu,et al. Gene. 206, 181-184(1998).
规格
规格
-
Catalog No.ON-109ON-109-G
-
SourceRecombinant E.coliRecombinant E.coli
-
Purity>95% by SDS-PAGE>95% by SDS-PAGE
-
Storage Condition-20℃-20℃
Documentation
Documentation
产品信息
DNase I(无 RNase)是一种内切核酸酶,可消化单链和双链 DNA。它能非特异性地切割 DNA,释放出具有 5' 磷酸化和 3' 羟基末端的二核苷酸、三核苷酸及寡核苷酸产物。
来源:重组大肠杆菌
浓度:1U/μL
单位定义:一个单位定义为在 37°C 下 10 分钟内完全降解 1μg λDNA 所需的酶量。
储存缓冲液:50mM Tris-HCl(pH7.5,25℃),10mM CaCl2,50% 甘油
配套产品:
操作要点
IVT 反应后的 DNase I 处理:
- 直接将 DNase I(每 μg DNA 模板加入 1 单位 DNase I)加入 IVT 反应液中,混匀后于 37°C 孵育 30 分钟。
- 加入 0.5M EDTA 至终浓度高于二价阳离子浓度 1 mM 以上。
- 75°C 加热 10 分钟使酶热失活。
安全信息
储存条件:-20℃
质量保证:无 RNase 活性污染。
物理纯度:SDS-PAGE 检测 >95%。
参考文献
1. A F Worrall,B A Connolly, J,Biol,Chem. 265,21889–21896 (1990).
2. Ching-Ying Chen,Shao-Chun Lu,et al. Gene. 206, 181-184(1998).
-
Catalog No.ON-109ON-109-G
-
SourceRecombinant E.coliRecombinant E.coli
-
Purity>95% by SDS-PAGE>95% by SDS-PAGE
-
Storage Condition-20℃-20℃
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