DNase I (RNase-free)

Catalog No. ON-109
Purity >95% by SDS-PAGE
Storage Condition -20℃
Source Recombinant E.coli

DNase I (RNase-free)

Grade
GMP level
Amount/Package: 1kU / centrifuge tube
SKU: ON-109-KU001-P
单价:
$25.00
Price
$25.00
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Products on this site:
Currently ships to US and Canada.
For shipping worldwide, please contact us.
Order fulfillment: within 2 business days, if available.
US: 2-8 days (from CA by FedEx).
Canada: 5-10 days (excludes customs delays).

Details

产品信息

DNase I(无 RNase)是一种内切核酸酶,可消化单链和双链 DNA。它能非特异性地切割 DNA,释放出具有 5' 磷酸化和 3' 羟基末端的二核苷酸、三核苷酸及寡核苷酸产物。

来源:重组大肠杆菌
浓度:1U/μL
单位定义:一个单位定义为在 37°C 下 10 分钟内完全降解 1μg λDNA 所需的酶量。
储存缓冲液:50mM Tris-HCl(pH7.5,25℃),10mM CaCl2,50% 甘油

配套产品:


操作要点

IVT 反应后的 DNase I 处理:

  1. 直接将 DNase I(每 μg DNA 模板加入 1 单位 DNase I)加入 IVT 反应液中,混匀后于 37°C 孵育 30 分钟。
  2. 加入 0.5M EDTA 至终浓度高于二价阳离子浓度 1 mM 以上。
  3. 75°C 加热 10 分钟使酶热失活。

安全信息

储存条件:-20℃
质量保证:无 RNase 活性污染。
物理纯度:SDS-PAGE 检测 >95%。


参考文献

1. A F Worrall,B A Connolly, J,Biol,Chem. 265,21889–21896 (1990).
2. Ching-Ying Chen,Shao-Chun Lu,et al. Gene. 206, 181-184(1998).

规格

  • Catalog No.
    ON-109
  • Source
    Recombinant E.coli
  • Purity
    >95% by SDS-PAGE
  • Storage Condition
    -20℃

Documentation

产品信息

DNase I(无 RNase)是一种内切核酸酶,可消化单链和双链 DNA。它能非特异性地切割 DNA,释放出具有 5' 磷酸化和 3' 羟基末端的二核苷酸、三核苷酸及寡核苷酸产物。

来源:重组大肠杆菌
浓度:1U/μL
单位定义:一个单位定义为在 37°C 下 10 分钟内完全降解 1μg λDNA 所需的酶量。
储存缓冲液:50mM Tris-HCl(pH7.5,25℃),10mM CaCl2,50% 甘油

配套产品:


操作要点

IVT 反应后的 DNase I 处理:

  1. 直接将 DNase I(每 μg DNA 模板加入 1 单位 DNase I)加入 IVT 反应液中,混匀后于 37°C 孵育 30 分钟。
  2. 加入 0.5M EDTA 至终浓度高于二价阳离子浓度 1 mM 以上。
  3. 75°C 加热 10 分钟使酶热失活。

安全信息

储存条件:-20℃
质量保证:无 RNase 活性污染。
物理纯度:SDS-PAGE 检测 >95%。


参考文献

1. A F Worrall,B A Connolly, J,Biol,Chem. 265,21889–21896 (1990).
2. Ching-Ying Chen,Shao-Chun Lu,et al. Gene. 206, 181-184(1998).

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