T3 DNA ligase
| Catalog No. | ON-1188 |
| Purity | ＞95% by SDS-PAGE |
| Storage Condition | -20℃ |
| Source | Recombinant E.coli |
T3 DNA ligase
T3 DNA ligase - M (Molecular Biology) / Non-GMP 缺货，一旦补货将尽快发货。
无法加载取货地点可用性
Shipping notes
Shipping notes
Details
Details
Product Information
T3 DNA Ligase is an ATP-dependent dsDNA ligase derived from bacteriophage T3 and recombinantly expressed in E. coli. It catalyzes the formation of phosphodiester bonds between adjacent 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA (dsDNA), with higher efficiency for A/T overhangs than C/G overhangs. The enzyme efficiently ligates dsDNA with either sticky or blunt ends, repairs nicks in dsDNA, and seals nicks in DNA/RNA hybrids containing an intact DNA strand. Consequently, T3 DNA Ligase can be used to generate DNA-RNA chimeras and to ligate RNA fragments using a DNA template.
Similar to T4 DNA Ligase, the addition of PEG 6000 significantly enhances blunt-end ligation efficiency. T3 DNA Ligase exhibits twice the salt tolerance of T4 DNA Ligase, retaining >95% activity in 1.0 M NaCl or KCl, making it an ideal choice for ligation under high-salt conditions.
规格
规格
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Catalog No.ON-1188
-
SourceRecombinant E.coli
-
Purity＞95% by SDS-PAGE
-
Storage Condition-20℃
Documentation
Documentation
Product Information
T3 DNA Ligase is an ATP-dependent dsDNA ligase derived from bacteriophage T3 and recombinantly expressed in E. coli. It catalyzes the formation of phosphodiester bonds between adjacent 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA (dsDNA), with higher efficiency for A/T overhangs than C/G overhangs. The enzyme efficiently ligates dsDNA with either sticky or blunt ends, repairs nicks in dsDNA, and seals nicks in DNA/RNA hybrids containing an intact DNA strand. Consequently, T3 DNA Ligase can be used to generate DNA-RNA chimeras and to ligate RNA fragments using a DNA template.
Similar to T4 DNA Ligase, the addition of PEG 6000 significantly enhances blunt-end ligation efficiency. T3 DNA Ligase exhibits twice the salt tolerance of T4 DNA Ligase, retaining >95% activity in 1.0 M NaCl or KCl, making it an ideal choice for ligation under high-salt conditions.
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Catalog No.ON-1188
-
SourceRecombinant E.coli
-
Purity＞95% by SDS-PAGE
-
Storage Condition-20℃
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