DNase I (RNase-free)
| Catalog No. | ON-109 |
| Storage Condition | -20℃ |
| Source | Recombinant E.coli |
| Purity | ＞95% by SDS-PAGE |
DNase I (RNase-free)
DNase I (RNase-free) - M (Molecular Biology) / Non-GMP / 1kU / centrifuge tube is backordered and will ship as soon as it is back in stock.
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Details
Details
Product Information
Product description: DNase I (RNase free) is an endonuclease that digests single- and double-stranded DNA. It nonspecifically cleaves DNA to release di-,tri- and oligonucleotide products with 5'-phosphorylated and 3'-hydroxylated end.
Source: Recombinant E.coli
Concentration: 1U/μL
Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg of λDNA in 10 minutes at 37℃.
Storage Buffer: 50mM Tris-HCl (pH7.5, 25℃), 10mM CaCl2 and 50% Glyercol
Companion Product: 10X DNase I Reaction Buffer, Cat#ON-077; 100mM Tris-HCl pH7.6, 25℃, 25mM MgCl2, 5mM CaCl2.
Key points of operation
DNase I treatment after IVT reaction:
1. Directly add DNase I (1unit DNase I per ug of DNA template) to IVT Reaction, mix well and incubate 30 min at 37°C.
2. Add 0.5M EDTA to a final concertation over one millimole than divalent cations.
3. Heat inactivate at 75℃ for 10min.
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
Reference
1. A F Worrall，B A Connolly, J，Biol，Chem. 265,21889–21896 (1990).
2. Ching-Ying Chen，Shao-Chun Lu，et al. Gene. 206, 181-184(1998).
Specifications
Specifications
-
Catalog No.ON-109ON-109-G
-
SourceRecombinant E.coliRecombinant E.coli
-
Purity＞95% by SDS-PAGE＞95% by SDS-PAGE
-
Storage Condition-20℃-20℃
Documentation
Documentation
Product Information
Product description: DNase I (RNase free) is an endonuclease that digests single- and double-stranded DNA. It nonspecifically cleaves DNA to release di-,tri- and oligonucleotide products with 5'-phosphorylated and 3'-hydroxylated end.
Source: Recombinant E.coli
Concentration: 1U/μL
Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg of λDNA in 10 minutes at 37℃.
Storage Buffer: 50mM Tris-HCl (pH7.5, 25℃), 10mM CaCl2 and 50% Glyercol
Companion Product: 10X DNase I Reaction Buffer, Cat#ON-077; 100mM Tris-HCl pH7.6, 25℃, 25mM MgCl2, 5mM CaCl2.
Key points of operation
DNase I treatment after IVT reaction:
1. Directly add DNase I (1unit DNase I per ug of DNA template) to IVT Reaction, mix well and incubate 30 min at 37°C.
2. Add 0.5M EDTA to a final concertation over one millimole than divalent cations.
3. Heat inactivate at 75℃ for 10min.
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
Reference
1. A F Worrall，B A Connolly, J，Biol，Chem. 265,21889–21896 (1990).
2. Ching-Ying Chen，Shao-Chun Lu，et al. Gene. 206, 181-184(1998).
-
Catalog No.ON-109ON-109-G
-
SourceRecombinant E.coliRecombinant E.coli
-
Purity＞95% by SDS-PAGE＞95% by SDS-PAGE
-
Storage Condition-20℃-20℃
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Purity: ＞95% by SDS-PAGE
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