Cap Analogs

(15 products)
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General structure of our Cap Analogs.

What are Cap Analogs?
Cap analogs are chemically modified nucleotides designed to structurally and functionally mimic the natural 5' cap structure (m⁷GpppN) of eukaryotic messenger RNA (mRNA). These synthetic molecules are utilized during in vitro transcription (IVT) to incorporate a 5' cap co-transcriptionally, eliminating the need for post-synthetic capping enzymes and producing homogeneous, properly capped mRNA transcripts.

Synthesis and Design
Our cap analogs are synthesized through sophisticated organic chemistry routes that allow for precise modifications:

  • Chemical Synthesis: The core structure, typically a modified guanine derivative (such as 7-methylguanosine / m7G), is chemically synthesized and activated to form dinucleotide cap analogs (e.g., m⁷GpppG).
  • Modification and Functionalization: We offer analogs with strategic modifications, including anti-reverse cap analogs (ARCA) for exclusive forward orientation, as well as affinity tags (e.g., biotin) or fluorescent labels for detection and purification.
  • Stringent QC: Each batch is rigorously purified (HPLC) and validated (mass spectrometry, NMR) to ensure high purity, correct stereochemistry, and optimal capping efficiency.

Usage in mRNA Synthesis
Cap analogs are included in IVT reactions alongside standard NTPs and an RNA polymerase (e.g., T7 RNA polymerase). They are incorporated at the 5' end of the nascent RNA chain, yielding mRNA that is:

  • Translationally Competent: Ready for efficient ribosome binding and initiation.
  • Stable: Resistant to decapping enzymes and 5' exonucleases.
  • Functionally Enhanced: Suitable for advanced applications requiring tracking, purification, or modulated translational activity.

Our Advantages

We provide cap analogs that ensure your mRNA transcripts achieve the highest levels of performance and reliability:

Enhanced Translational Efficiency

Our cap analogs, including our high-performance ARCA variants, ensure proper cap orientation, leading to significantly improved protein yields in cell-free systems and living cells.

Superior mRNA Stability

Capping with our analogs protects mRNA from degradation, extending its functional half-life and increasing the duration and magnitude of gene expression.

Customization and Innovation

We offer a range of specialized analogs, including fluorescently labeled and cleavable caps, enabling novel applications in tracking, purification, and controlled expression.

Exceptional Batch Consistency

Our stringent manufacturing and quality control processes guarantee high purity and reliable performance in every order, ensuring reproducible experimental results.

Applications of Our Cap Analogs

By leveraging deep expertise in nucleic acid chemistry and mRNA biology, we provide cap analogs that ensure the integrity, stability, and functionality of your mRNA constructs across multiple fields:

mRNA-Based Therapeutics and Vaccines

  1. Vaccine Development: Production of capped mRNA for vaccination, ensuring robust antigen expression and immune activation.
  2. Protein Replacement Therapy: Synthesis of therapeutic mRNA encoding functional proteins for treatment of genetic disorders.

Basic and Applied Research

  1. In Vitro and In Vivo Translation Studies: Generation of capped mRNA for mechanistic studies of translation initiation in cell-free extracts, cultured cells, or microinjected model organisms.
  2. Gene Function Analysis: mRNA cap analogs enable functional studies of gene expression and regulation in systems where DNA transfection is not optimal.

Biotechnology and Synthetic Biology

  1. Cell-Free Protein Synthesis: High-yield production of proteins in reconstituted transcription-translation systems for research and diagnostic purposes.
  2. Engineered mRNA for Delivery Systems: Design of capped mRNA for use in lipid nanoparticles (LNPs) or other delivery vehicles for targeted interventions.

Specialized Detection and Tracking

  1. Visualization and Purification: Incorporation of tagged cap analogs (e.g., biotinylated or fluorescent caps) allows for detection, imaging, and affinity purification of synthesized mRNA.
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