General structure of PNA Monomers, two different groups
What are PNA Monomers? PNA (Peptide Nucleic Acid) monomers are specialized molecular units used to construct PNA oligomers—synthetic analogs of DNA/RNA that replace the natural sugar-phosphate backbone with a structurally robust N-(2-aminoethyl)glycine peptide backbone. Each monomer consists of a nucleobase (adenine, thymine, cytosine, guanine, or modified variants) linked to this pseudopeptide backbone, enabling sequence-specific hybridization with complementary DNA and RNA strands with higher affinity and specificity than natural oligonucleotides.
Synthesis and Oligomer Assembly Our PNA monomers are synthesized and validated to the highest standards to ensure optimal performance in oligomer construction:
Chemical Synthesis: PNA monomers are produced using advanced solid-phase peptide synthesis (SPPS) techniques. Each monomer is designed with protective groups (e.g., Fmoc for the backbone amine, and appropriate nucleobase protection) to allow iterative, controlled chain elongation.
Solid-Phase PNA Oligomer Synthesis: PNA oligos are assembled on a solid support (e.g., resin beads) using automated synthesizers:
Deprotection: Removal of the Fmoc group from the terminal amine.
Coupling: Activation and addition of the next PNA monomer.
Capping: Acetylation of unreacted chains to minimize deletion sequences.
Cleavage and Deprotection: Final oligos are cleaved from the resin, and side-chain protecting groups are removed under optimized conditions.
Quality Control: All monomers are rigorously analyzed via HPLC and mass spectrometry to ensure ≥97% purity, batch-to-batch consistency, and high coupling efficiency.
Our Advantages
We deliver PNA monomers that empower researchers to achieve superior experimental outcomes:
Exceptional Monomer Purity & Reactivity
Our monomers are optimized for high coupling efficiency, enabling the synthesis of long and complex PNA sequences with excellent yield and minimal by-products.
Enhanced Oligomer Performance
NAs synthesized from our monomers exhibit stronger binding affinity (e.g., higher Tm values) and greater specificity toward complementary DNA/RNA targets, even under challenging conditions.
Chemical Stability
PNA oligos built with our monomers demonstrate superior resistance to nucleases and proteases, making them ideal for applications in complex biological environments.
Customization Capabilities
We offer standard and modified nucleobases (e.g., labeled, alkylated, or fluorescent monomers), backbone variations, and bulk quantities to support diverse research and therapeutic goals.
Applications of Our Succinates
PNA oligos synthesized from our monomers are transforming research and innovation across multiple fields:
Molecular Diagnostics & Detection
Fluorescent In Situ Hybridization (FISH): PNA probes enable high-resolution, specific detection of chromosomal abnormalities, pathogens, or gene expression patterns in cells and tissues.
PCR Clamping and Mutation Detection: PNAs suppress amplification of wild-type sequences, allowing sensitive identification of rare mutations in cancer and genetic disorders.
Biosensors: Integrated into platforms for detecting nucleic acids with high specificity in clinical, environmental, or food safety testing.
Basic Research & Tool Development
Gene Editing and Regulation: Used as steric blockers or decoy molecules to modulate transcription, translation, and splicing mechanisms in cell-free and cellular systems.
Nanotechnology and Bioconjugation: PNAs serve as programmable building blocks in nano-assemblies and drug delivery systems due to their predictable hybridization and functionalization properties.
Therapeutic Development
Antisense and Antigene Therapy: PNA oligos inhibit gene expression by binding to mRNA or genomic DNA, offering a potent strategy for treating genetic diseases, cancers, and viral infections.
Anti-Microbial Agents: PNAs target essential bacterial or fungal genes, providing a novel approach to combat antimicrobial resistance.
Synthetic Biology
Directed Evolution and Screening: PNA-mediated sequence perturbation enables innovative approaches in protein engineering and functional genomics.
