Solid Supports

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What are Solid Supports?
Solid supports, commonly referred to as resins, are insoluble, functionalized polymeric or inorganic particles that serve as the foundational substrate for solid-phase oligonucleotide synthesis (SPOS). These materials typically range from 50 to 200 micrometers in diameter and provide a stable, high-surface-area anchor onto which the oligonucleotide chain is assembled step-by-step using phosphoramidite chemistry. The choice of support directly impacts synthesis scale, efficiency, and final oligo quality.

Primary Types and Synthesis Functionalization:

  • Controlled Pore Glass (CPG): The industry benchmark, CPG is a rigid, silica-based material featuring a highly controlled network of pores. This structure ensures efficient diffusion of synthesis reagents, enabling high coupling efficiencies—especially for long or complex sequences. CPG supports are typically functionalized with linkers (e.g., succinyl, aminoalkyl) that covalently bind the 3′-end of the initial nucleoside.
  • Polystyrene (PS) Supports: A cost-effective organic polymer option, often used in large-scale synthesis. To mitigate inherent issues like swelling in organic solvents (which can reduce reagent accessibility and coupling consistency), our polystyrene supports are engineered with controlled cross-linking to improve dimensional stability.

Role in Oligonucleotide Synthesis:
During synthesis, the solid support is packed into a column where cyclic chemical steps—deprotection, coupling, capping, and oxidation—are performed. Its key functions include:

  • Immobilizing the growing oligonucleotide chain.
  • Enabling efficient reagent access and by-product removal.
  • Allowing final cleavage and deprotection under specific conditions to release the desired oligo.

Our Advantages

We provide solid supports that deliver superior synthesis performance, ensuring reliability and value across all your oligonucleotide production needs:

High Loading Capacity & Uniform Functionalization

Our CPG and polystyrene supports offer consistent and high-density functional groups, maximizing yield and synthesis efficiency while reducing failure sequences.

Optimized Physical & Chemical Stability

Our CPG demonstrates exceptional mechanical and chemical resistance, while our advanced polystyrene formulations minimize swelling/shrinkage, ensuring reproducible results across diverse sequences and scales.

Extensive Portfolio for Every Need

We offer a wide selection of pore sizes (e.g., 500Å, 1000Å, 2000Å), functional groups, and support materials tailored to applications ranging from small-scale research to large-scale therapeutic production.

Proven Performance in Demanding Syntheses

Engineered to support the synthesis of long oligonucleotides, modified oligos, and complex sequences such as RNA and antisense oligonucleotides (ASOs) with high fidelity.

Applications of Our Solid Supports

Our solid supports are essential to a broad spectrum of oligonucleotide-based applications across research, diagnostics, and therapeutics:

Research-Grade Oligonucleotide Synthesis

  1. PCR Primers and Probes: Production of high-purity primers and probes for genetic analysis, cloning, and real-time PCR.
  2. Next-Generation Sequencing (NGS): Synthesis of adapters, barcodes, and other oligos used in library preparation.
  3. Gene Editing: Manufacture of CRISPR guide RNAs (gRNAs) and donor DNA templates.

Therapeutic Oligonucleotide Manufacturing

  1. Antisense Oligonucleotides (ASOs) and siRNA: Large-scale synthesis of therapeutic oligonucleotides using supports designed for Good Manufacturing Practice (GMP) compliance.
  2. Aptamer Development: Generation of structured nucleic acid ligands for targeted therapeutics and diagnostics.

Diagnostic Assay Development

  1. Microarrays: Functionalized supports enable in-situ synthesis of high-density oligonucleotide arrays for genomics and transcriptomics.
  2. Biosensors: Production of capture probes for electrochemical and optical nucleic acid sensors.

Chemical Biology and Bioconjugation

  1. Functionalized Oligos: Supports pre-functionalized with specific groups (e.g., amines, thiols) facilitate the post-synthesis conjugation of dyes, peptides, or small molecules.
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