T7 RNA polymerase

Catalog No. ON-004-C050
Purity ＞95% by SDS-PAGE
Storage Condition -20℃
Source Recombinant E.coli

T7 RNA polymerase

GMP level
Concentration
Amount/Package: 10kU / centrifuge tube
SKU: ON-004-C050-KU010-P
Collections:  Biochemical Reagents Enzyme & Related Buffer for mRNA Enzymes Enzymes & Buffers Enzymes for mRNA Synthesis mRNA Other Raw Materials RNA Synthesis
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$25.00

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Details

Product Information

T7 RNA Polymerase is a monomeric bacteriophage encoded DNA directed RNA polymerase which catalyzes the formation of RNA in the 5'-3' direction. In the process of initiation of transcription T7 recognizes a specific promoter sequence, the T7 promoter. T7 consists of 883 amino acids and has a molecular weight of 99kDa.

Source: Recombinant E.coli
Concentration: 50/200/1000U/μL (KU/mL)
Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of rATP into acid-insoluble product in 1 hour at 37℃.
Storage Buffer: 20mM Tris-HCl PH 7.9, 100mM NaCl, 10mM DTT, 0.1% Triton X-100, 1mM EDTA, 50%(v/v) Glycerol.

Companion Products:

Cat#ON-062 10X IVT Reaction Buffer inside with:
- 400mM Tris PH7.9
- 100mM DTT
- 20mM Spermidine
- 60mM MgCl2
Cat#ON-041 10X IVT Reaction Buffer without Mg2+ inside with:
- 400mM Tris PH7.9
- 100mM DTT
- 20mM Spermidine.

Key points of operation

  1. T7 RNA Polymerase is extremely sensitive to salt inhibition. For best overall salt concentration should not exceed 50mM.
  2. Higher yields of RNA may be obtained by raising NTP concentrations (up to 10mM each). Mg2+ concentration should be raised to 4~10mM above the total NTP concentration. T7 RNA Polymerase should be raised to 30K units/mL. Additionally, inorganic pyrophosphatase should be added to a final concentration of 4 units/mL.
  3. An apparent decrease in enzyme activity over time may be due to the breakdown of dithiothreitol in the reaction buffer; even when stored at –20°C. If you observe a decrease in yield, try supplementing your reactions with a final concentration of 20mM fresh dithiothreitol. Or make a reaction buffer without DTT and add fresh DTT when preparing reaction mixture.

Security Information

Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.

Reference

1. Chamberlin, M., McGrath, J. & Waskell, L. Nature 228, 227–31 (1970).
2. Davanloo, P., Rosenberg, A. H., Dunn, J. J. & Studier, F. W. Proc. Natl. Acad. Sci. U. S. A. 81, 2035–9 (1984).

Specifications
  • Catalog No.
    ON-004-C050
  • Source
    Recombinant E.coli
  • Purity
    ＞95% by SDS-PAGE
  • Storage Condition
    -20℃

Documentation
COA Contact Us
Spec Download

Product Information

T7 RNA Polymerase is a monomeric bacteriophage encoded DNA directed RNA polymerase which catalyzes the formation of RNA in the 5'-3' direction. In the process of initiation of transcription T7 recognizes a specific promoter sequence, the T7 promoter. T7 consists of 883 amino acids and has a molecular weight of 99kDa.

Source: Recombinant E.coli
Concentration: 50/200/1000U/μL (KU/mL)
Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of rATP into acid-insoluble product in 1 hour at 37℃.
Storage Buffer: 20mM Tris-HCl PH 7.9, 100mM NaCl, 10mM DTT, 0.1% Triton X-100, 1mM EDTA, 50%(v/v) Glycerol.

Companion Products:

Cat#ON-062 10X IVT Reaction Buffer inside with:
- 400mM Tris PH7.9
- 100mM DTT
- 20mM Spermidine
- 60mM MgCl2
Cat#ON-041 10X IVT Reaction Buffer without Mg2+ inside with:
- 400mM Tris PH7.9
- 100mM DTT
- 20mM Spermidine.

Key points of operation

  1. T7 RNA Polymerase is extremely sensitive to salt inhibition. For best overall salt concentration should not exceed 50mM.
  2. Higher yields of RNA may be obtained by raising NTP concentrations (up to 10mM each). Mg2+ concentration should be raised to 4~10mM above the total NTP concentration. T7 RNA Polymerase should be raised to 30K units/mL. Additionally, inorganic pyrophosphatase should be added to a final concentration of 4 units/mL.
  3. An apparent decrease in enzyme activity over time may be due to the breakdown of dithiothreitol in the reaction buffer; even when stored at –20°C. If you observe a decrease in yield, try supplementing your reactions with a final concentration of 20mM fresh dithiothreitol. Or make a reaction buffer without DTT and add fresh DTT when preparing reaction mixture.

Security Information

Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.

Reference

1. Chamberlin, M., McGrath, J. & Waskell, L. Nature 228, 227–31 (1970).
2. Davanloo, P., Rosenberg, A. H., Dunn, J. J. & Studier, F. W. Proc. Natl. Acad. Sci. U. S. A. 81, 2035–9 (1984).

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We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

Manufacturing Scalability

Hongene operates a 1.67-million-square-foot oligonucleotide manufacturing facility featuring advanced equipment, including multiple OligoPilot™ and OligoProcess™ synthesizers ranging from 10 to 1,800 mmol. Its 48 flexible production lines enable seamless, one-stop scale-up of API production from gram-level quantities to metric tons, achieving purity of ≥98% while supporting compliance with NMPA, FDA, and EMA regulatory requirements.

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Global Business Distribution
40+
Countries & Regions
≈3000
Global Clients
54000L+
Annual NTP Production
58t+
Annual Amidites Production

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