Taq DNA Ligase

Catalog No. ON-308
Purity ＞95% by SDS-PAGE
Storage Condition -20℃
Source Recombinant E.coli

Taq DNA Ligase

Grade
SKU: ON-308
Collections:  Biochemical Reagents Diagnostics Enzyme & Related Buffer for Diagnostics Enzymes Enzymes & Buffers Enzymes for Diagnostics Other Raw Materials PCR
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Details

Product Information

Product description: Taq DNA ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD+ as a cofactor.

Source: Recombinant E.coli

Concentration: 40U/μL

Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1μg of BstEII-digested λDNA in a total reaction volume of 50μL in 15minutes at 45℃.

Storage Buffer: 10mM Tris-HCl (pH 7.5, 25℃), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1% Triton X-100 and 50% (v/v)Glyercol.

Companion Product: 10X Taq DNA Ligase Reaction Buffer, Cat#ON-072, 200mM Tris-HCl pH 7.6, 25℃, 250mM KAC, 100mM Mg(AC)2, 10mM NAD+,0.1% Triton X-100.

Key points of operation

Note: The ligation reaction requires NAD+ as a cofactor. NAD+ is supplied in the 10×Taq DNA Ligase Reaction Buffer, the buffer should be stored at -70℃ to extend the half life of the NAD+ cofactor. Taq DNA Ligase is active at 45-65℃

Recommended Protocol:

  1. Add the following reagents in a PCR tube:
Component
Amount
Nuclease-free H2O
to 50μL
DNA
up to 1μg of DNA
10X Taq DNA Ligase Reaction Buffer
5μL
Taq DNA Ligase
2μL (80 units)

  1. Incubate at 45℃ for 15 min.

Security Information

Storage Conditions: -20℃

Quality Assurance: Free of endonuclease, exonuclease, and Nickase activities.

Physical Purity: ＞95% by SDS-PAGE.

Reference

1. Barany F. Proc Nat Acad Sci USA. 88, 189–193 (1991).

2. Gail Lauer, Edwin A. Rudd, Dianel L. Mckay, et al. Journal Of Bacteriology. 173, 5047-5053(1991).

Specifications
  • Catalog No.
    ON-308
  • Source
    Recombinant E.coli
  • Purity
    ＞95% by SDS-PAGE
  • Storage Condition
    -20℃

Documentation
COA Contact Us

Product Information

Product description: Taq DNA ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD+ as a cofactor.

Source: Recombinant E.coli

Concentration: 40U/μL

Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1μg of BstEII-digested λDNA in a total reaction volume of 50μL in 15minutes at 45℃.

Storage Buffer: 10mM Tris-HCl (pH 7.5, 25℃), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1% Triton X-100 and 50% (v/v)Glyercol.

Companion Product: 10X Taq DNA Ligase Reaction Buffer, Cat#ON-072, 200mM Tris-HCl pH 7.6, 25℃, 250mM KAC, 100mM Mg(AC)2, 10mM NAD+,0.1% Triton X-100.

Key points of operation

Note: The ligation reaction requires NAD+ as a cofactor. NAD+ is supplied in the 10×Taq DNA Ligase Reaction Buffer, the buffer should be stored at -70℃ to extend the half life of the NAD+ cofactor. Taq DNA Ligase is active at 45-65℃

Recommended Protocol:

  1. Add the following reagents in a PCR tube:
Component
Amount
Nuclease-free H2O
to 50μL
DNA
up to 1μg of DNA
10X Taq DNA Ligase Reaction Buffer
5μL
Taq DNA Ligase
2μL (80 units)

  1. Incubate at 45℃ for 15 min.

Security Information

Storage Conditions: -20℃

Quality Assurance: Free of endonuclease, exonuclease, and Nickase activities.

Physical Purity: ＞95% by SDS-PAGE.

Reference

1. Barany F. Proc Nat Acad Sci USA. 88, 189–193 (1991).

2. Gail Lauer, Edwin A. Rudd, Dianel L. Mckay, et al. Journal Of Bacteriology. 173, 5047-5053(1991).

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Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

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We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

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