5X Reaction Buffer*
200μL
-20℃
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High Yield T7 RNA Synthesis Kit
The kit contains sufficient reagents for 50 reaction of 20μL each.
| Catalog No. | ON-040 |
| Storage Condition | -20℃ |
| Source | N/A |
| Purity | N/A |
High Yield T7 RNA Synthesis Kit
The kit contains sufficient reagents for 50 reaction of 20μL each.
Catalog No: ON-040
High Yield T7 RNA Synthesis Kit - M (Molecular Biology) / 1 Kit is backordered and will ship as soon as it is back in stock.
SKU:
ON-040-1K
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Details
Details
Product Information
The High Yield T7 RNA Synthesis Kit is designed to produce 25-fold more full-length RNA transcript per reaction than conventional in vitro transcription reactions. The yield is up to 210μg RNA per reaction.
Materials provided with the kit:
Component
50 rxn kits
Storage
100mM ATP Solution
100μL
-20℃
100mM GTP Solution
100μL
-20℃
100mM UTP Solution
100μL
-20℃
100mM CTP Solution
100μL
-20℃
Enzyme Mix
75μL
-20℃
Control Template (0.5μg/μL)
10μL
-20℃
DNase I (RNase-free, 1U/μL)
50μL
-20℃
Nuclease-free H2O
1mL
-20℃
Ammonium Acetate Stop Solution
1.5mL
-20℃
Lithium Chloride Precipitation Solution
1.5mL
-20℃
Gel Loading Buffer
0.5mL
-20℃
The kit contains sufficient reagents for 50 reaction of 20μL each.
Key points of operation
Note: 5X Reaction Buffer store at -70℃ may result in the formation of a white precipitate. If this happens, heat the tube to 37℃ for 5 minutes and mix thoroughly to resuspend the precipitate.
- The following amounts are for a single 20 μL reaction. Reactions may be scaled up or down if desired.
Component
Amount
Nuclease-free H2O
to 20μL
100mM ATP Solution
2μL
100mM GTP Solution
2μL
100mM UTP Solution
2μL
100mM CTP Solution
2μL
Template DNA
1μg*
5X Reaction Buffer
4μL
Enzyme Mix
1.5μL
* The transcript may vary depending on the amount of template DNA.
- Mix thoroughly and incubate 2h at 37℃.
- (optional) Add 1μL DNase I (RNase-free), mix well and incubate 30min at 37°C.
Security Information
Storage Conditions: -20℃
Quality Assurance: All components are tested in a functional assay as described in this procedure. A 20μL reaction containing 1 μg of the control template DNA which codes for a ~800b transcript synthesized >150μg of RNA after a 2 hr incubation.
Reference
1. Milligan JF, Groebe DR, Witherell GW, and Uhlenbeck OC (1987) Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA template. Nucl. Acids Res. 15: 8783–8798.
2. Molecular Cloning, A Laboratory Manual, 2nd edition. (1989) editor C Nolan, Cold Spring Harbor Laboratory Press.
Specifications
Specifications
-
Catalog No.ON-040
-
SourceN/A
-
PurityN/A
-
Storage Condition-20℃
Documentation
Documentation
FAQ
FAQ
What is the T7 In Vitro Transcription Kit used for?
What is the T7 In Vitro Transcription Kit used for?
The T7 In Vitro Transcription Kit is designed for the efficient synthesis of RNA transcripts from DNA templates containing a T7 promoter sequence. It is widely used in various applications, including RNA structure and function studies, ribozyme production, RNA probe generation for hybridization, RNA interference (RNAi) experiments, and in vitro translation studies.
What components are included in the T7 In Vitro Transcription Kit?
What components are included in the T7 In Vitro Transcription Kit?
Our T7 In Vitro Transcription Kit typically includes the following components:
- T7 RNA Polymerase
- Reaction Buffer (10x)
- NTP Mix (ATP, CTP, GTP, UTP)
- DTT (Dithiothreitol)
- RNase-Free Water
- Control Template DNA
- Instructions for Use
- Some kits may also include additional components such as DNase-Free RNase-Free Water, Magnesium Chloride, and RNase-Free Tubes.
- T7 RNA Polymerase
- Reaction Buffer (10x)
- NTP Mix (ATP, CTP, GTP, UTP)
- DTT (Dithiothreitol)
- RNase-Free Water
- Control Template DNA
- Instructions for Use
- Some kits may also include additional components such as DNase-Free RNase-Free Water, Magnesium Chloride, and RNase-Free Tubes.
What is the expected yield of RNA using the T7 In Vitro Transcription Kit?
What is the expected yield of RNA using the T7 In Vitro Transcription Kit?
The yield of RNA can vary depending on the template and reaction conditions. Typically, our T7 In Vitro Transcription Kit can produce up to 100–200 µg of RNA per 20 µl reaction, using a high-quality DNA template. For example, the HiScribe T7 High Yield RNA Synthesis Kit from NEB can produce up to 180 µg of RNA from 1 µg of control template in a standard 20 µl reaction.
Product Information
The High Yield T7 RNA Synthesis Kit is designed to produce 25-fold more full-length RNA transcript per reaction than conventional in vitro transcription reactions. The yield is up to 210μg RNA per reaction.
Materials provided with the kit:
Component
50 rxn kits
Storage
5X Reaction Buffer*
200μL
-20℃
100mM ATP Solution
100μL
-20℃
100mM GTP Solution
100μL
-20℃
100mM UTP Solution
100μL
-20℃
100mM CTP Solution
100μL
-20℃
Enzyme Mix
75μL
-20℃
Control Template (0.5μg/μL)
10μL
-20℃
DNase I (RNase-free, 1U/μL)
50μL
-20℃
Nuclease-free H2O
1mL
-20℃
Ammonium Acetate Stop Solution
1.5mL
-20℃
Lithium Chloride Precipitation Solution
1.5mL
-20℃
Gel Loading Buffer
0.5mL
-20℃
The kit contains sufficient reagents for 50 reaction of 20μL each.
Key points of operation
Note: 5X Reaction Buffer store at -70℃ may result in the formation of a white precipitate. If this happens, heat the tube to 37℃ for 5 minutes and mix thoroughly to resuspend the precipitate.
- The following amounts are for a single 20 μL reaction. Reactions may be scaled up or down if desired.
Component
Amount
Nuclease-free H2O
to 20μL
100mM ATP Solution
2μL
100mM GTP Solution
2μL
100mM UTP Solution
2μL
100mM CTP Solution
2μL
Template DNA
1μg*
5X Reaction Buffer
4μL
Enzyme Mix
1.5μL
* The transcript may vary depending on the amount of template DNA.
- Mix thoroughly and incubate 2h at 37℃.
- (optional) Add 1μL DNase I (RNase-free), mix well and incubate 30min at 37°C.
Security Information
Storage Conditions: -20℃
Quality Assurance: All components are tested in a functional assay as described in this procedure. A 20μL reaction containing 1 μg of the control template DNA which codes for a ~800b transcript synthesized >150μg of RNA after a 2 hr incubation.
Reference
1. Milligan JF, Groebe DR, Witherell GW, and Uhlenbeck OC (1987) Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA template. Nucl. Acids Res. 15: 8783–8798.
2. Molecular Cloning, A Laboratory Manual, 2nd edition. (1989) editor C Nolan, Cold Spring Harbor Laboratory Press.
-
Catalog No.ON-040
-
SourceN/A
-
PurityN/A
-
Storage Condition-20℃
What is the T7 In Vitro Transcription Kit used for?
What is the T7 In Vitro Transcription Kit used for?
The T7 In Vitro Transcription Kit is designed for the efficient synthesis of RNA transcripts from DNA templates containing a T7 promoter sequence. It is widely used in various applications, including RNA structure and function studies, ribozyme production, RNA probe generation for hybridization, RNA interference (RNAi) experiments, and in vitro translation studies.
What components are included in the T7 In Vitro Transcription Kit?
What components are included in the T7 In Vitro Transcription Kit?
Our T7 In Vitro Transcription Kit typically includes the following components:
- T7 RNA Polymerase
- Reaction Buffer (10x)
- NTP Mix (ATP, CTP, GTP, UTP)
- DTT (Dithiothreitol)
- RNase-Free Water
- Control Template DNA
- Instructions for Use
- Some kits may also include additional components such as DNase-Free RNase-Free Water, Magnesium Chloride, and RNase-Free Tubes.
- T7 RNA Polymerase
- Reaction Buffer (10x)
- NTP Mix (ATP, CTP, GTP, UTP)
- DTT (Dithiothreitol)
- RNase-Free Water
- Control Template DNA
- Instructions for Use
- Some kits may also include additional components such as DNase-Free RNase-Free Water, Magnesium Chloride, and RNase-Free Tubes.
What is the expected yield of RNA using the T7 In Vitro Transcription Kit?
What is the expected yield of RNA using the T7 In Vitro Transcription Kit?
The yield of RNA can vary depending on the template and reaction conditions. Typically, our T7 In Vitro Transcription Kit can produce up to 100–200 µg of RNA per 20 µl reaction, using a high-quality DNA template. For example, the HiScribe T7 High Yield RNA Synthesis Kit from NEB can produce up to 180 µg of RNA from 1 µg of control template in a standard 20 µl reaction.
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