Linkers

(105)

What are Linkers?
Linkers, also known as spacers or conjugation arms, are specialized molecular entities integrated during oligonucleotide synthesis to serve two primary functions:

Solid-Phase Attachment: To tether the oligonucleotide to a solid support (e.g., CPG, polystyrene beads) during synthesis.
Bioconjugation: To provide a functional handle for post-synthetic modification with dyes, proteins, nanoparticles, or other molecules.

These constructs are chemically designed to offer stability during synthesis and selective cleavability or conjugation under specific conditions.

Synthesis and Integration
Linkers are incorporated into oligonucleotides using phosphoramidite chemistry—the industry standard for automated oligo synthesis:

Functionalization: Solid supports are pre-functionalized with linkers (e.g., succinyl, long-chain alkylamine, or specialty cleavable groups) to allow initiation of oligo elongation.
Site-Specific Incorporation: During synthesis, linker phosphoramidites can be introduced at either the 5' or 3' end, or internally, to facilitate downstream conjugation.
Cleavage & Deprotection: After synthesis, specific treatments (e.g., ammonium hydroxide for base-labile linkers, UV for photolabile linkers, or enzymatic cleavage) are used to release the oligo or expose functional groups.

Our linkers are synthesized under strict QC protocols, including HPLC and MS validation, to ensure high coupling efficiency and batch-to-batch reproducibility.

Our Advantages

We provide linkers that enhance the performance, yield, and applicability of your oligonucleotide-based products:

High Purity

Our linkers could reach purity ≥98% — ensure reliable experimental results, reduce product failure risks in key applications, lower post-synthesis costs, and boost safety/compliance.

Broad Portfolio

From standard succinate and C6 linkers to advanced ortho-cleavable, azide, DBCO, and PEG-based spacers, we offer the industry’s most diverse selection.

Low Background & High Stability

Engineered to minimize nonspecific binding and degradation, our linkers are ideal for sensitive applications like sequencing and diagnostic assays.

Customization Ready

We tailor linker length, reactivity, and functional groups (e.g., thiol, amine, carboxyl) to suit your conjugation needs—enabling unique oligo constructs.

Scale-Up Support

We supply linkers from milligram to kilogram scale with no compromise on purity or performance, supporting projects from research to therapeutic development.

Applications of Our Linkers

Leveraging deep expertise in photochemistry and biofunctionalization, we provide dyes and quenchers precursors that bring clarity, control, and innovation to scientific and medical advancements:

Diagnostic Assays & Biosensors

qPCR Probes: Probes with quencher-dye pairs connected via nuclease-sensitive linkers.
Microarrays: Non-cleavable linkers immobilize oligos on glass slides with uniform orientation and accessibility.
Lateral Flow Tests: Biotin and carboxy linkers facilitate antibody-oligo conjugates for rapid detection.

Therapeutic Development

siRNA & Antisense Oligonucleotides: Linkers incorporate conjugation handles for GalNAc targeting ligands, enhancing tissue-specific delivery.
mRNA Therapeutics: Click chemistry-compatible linkers (e.g., azide-alkyne) allow precise capping and modification.
Antibody-Drug Conjugates (ADCs): Oligo-linker systems enable controlled payload release in targeted therapies.

Genomics & Sequencing

NGS Library Prep: Cleavable adapters and barcode linkers improve multiplexing efficiency and read accuracy.
Single-Cell Sequencing: Photo-cleavable linkers enable spatial transcriptomics and cell-specific barcode release.

Imaging & Detection

FISH Probes: Fluorophore-conjugated linkers help visualize genetic loci with high signal-to-noise ratio.
FRET-Based Systems: Spacer linkers of precise lengths optimize energy transfer between fluorophores.

Chemical Biology & Drug Discovery

PROTACs & Bifunctional Molecules: Linkers serve as structural components that join oligo binders with effector domains.
Activity-Based Probes: Enzyme-cleavable linkers report on catalytic activity in real time.