DMT-2'-O-MOE-T-CE-Phosphoramidite

5-methyl-5'-O-(4, 4'-dimethoxytrityl)-2'-O-methoxyethyl-uridine-3'-cyanoethyl Phosphoramidite
Catalog No. PR4-002
CAS No. 163878-63-5
Purity HPLC≥98.0%
Molecular Formula C43H55N4O10P
Molecular Weight 818.90
Storage Condition -20℃

DMT-2'-O-MOE-T-CE-Phosphoramidite

5-methyl-5'-O-(4, 4'-dimethoxytrityl)-2'-O-methoxyethyl-uridine-3'-cyanoethyl Phosphoramidite
Grade
Amount/Package: 0.25g / 30mL screwed bottle-28
SKU: PR4-002-025A  |  suitable for  MerMade
Collections:  2'-O-MOE Phosphoramidites Oligo Synthesis Phosphoramidites Synthesis Blocks
Unit price:
$15.00

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Details

DMT-2'-O-MOE-T-CE-Phosphoramidite is a 2'-MOE phosphoramidite monomer used for solid-phase oligonucleotide synthesis. It contains a 5'-dimethoxytrityl (DMT) protecting group for 5'-hydroxyl protection, a thymine, a cyanoethyl (CE) protecting group on phosphite, and a 2'-O-methoxyethyl (MOE) modification.

2'-O-MOE provide enhanced nuclease resistance, duplex stability, and low cytotoxicity compared with unmodified RNA.

Structurally, the 2'-hydroxyl group of ribose is replaced by a –O–CH₂–CH₂–O–CH₃ (methoxyethyl) group, which locks the sugar into an RNA-like C3'-endo conformation and stabilizes hybrid formation with complementary RNA strands.

Applications

RNase H-dependent antisense oligonucleotides (ASO)

Often as 2'-O-MOE/DNA chimeras that activate RNase H-mediated cleavage of target RNA.[1]

Steric-blocking ASO

Physically obstructing access to specific RNA molecules, rather than causing their degradation, e.g., splice-switching ASO.[1]

Enzyme or structural RNA inhibition

Inhibit specific RNAs or enzymes (e.g., telomerase) through binding interference.[2]

Features and Benefits

High coupling efficiency and reproducibility in solid-phase DNA synthesis
High purity (≥ 98%) and tightly controlled impurity profiles
Compatible with standard DNA/RNA phosphoramidites
Simplified purification: no special precautions needed against RNase degradation
Fast-deprotection variants available upon request

Other Notes

  • Storage: Store in a dry, inert atmosphere at -20 °C.
  • Coupling: 6 minute coupling time recommended
  • Recommended deprotection: 8 h at 55 °C in concentrated ammonia, or 10 min at 65 °C in AMA (ammonia/methylamine, 1:1 v/v)
  • Compatibility: Can be used alongside modified phosphoramidites (e.g., 2′-OMe, 2′-MOE, Locked-NA) to synthesize chimeric oligonucleotides.

Reference

[1] Hill, Alyssa C., and Jonathan Hall. "The MOE modification of RNA: Origins and widescale impact on the oligonucleotide therapeutics field." Helvetica Chimica Acta 106, no. 3 (2023): e202200169.
[2] Elayadi, Anissa N., Andrea Demieville, Edward V. Wancewicz, Brett P. Monia, and David R. Corey. "Inhibition of telomerase by 2′-O-(2-methoxyethyl) RNA oligomers: effect of length, phosphorothioate substitution and time inside cells." Nucleic Acids Research 29, no. 8 (2001): 1683-1689.

Specifications
  • Catalog No.
    PR4-002
  • CAS No.
    163878-63-5
  • SMILES
    CC(C)N(C(C)C)P(OCCC#N)O[C@H]1[C@@H](OCCOC)[C@H](N(C=C2C)C(NC2=O)=O)O[C@@H]1COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5
  • Molecular Formula
    C43H55N4O10P
  • Molecular Weight
    818.90
  • Appearance
    White to off-white powder
  • Purity
    HPLC≥98.0%
  • Storage Condition
    -20℃
  • Moisture Content
    K.F.≤0.30% w/w

Documentation
COA Contact Us

DMT-2'-O-MOE-T-CE-Phosphoramidite is a 2'-MOE phosphoramidite monomer used for solid-phase oligonucleotide synthesis. It contains a 5'-dimethoxytrityl (DMT) protecting group for 5'-hydroxyl protection, a thymine, a cyanoethyl (CE) protecting group on phosphite, and a 2'-O-methoxyethyl (MOE) modification.

2'-O-MOE provide enhanced nuclease resistance, duplex stability, and low cytotoxicity compared with unmodified RNA.

Structurally, the 2'-hydroxyl group of ribose is replaced by a –O–CH₂–CH₂–O–CH₃ (methoxyethyl) group, which locks the sugar into an RNA-like C3'-endo conformation and stabilizes hybrid formation with complementary RNA strands.

Applications

RNase H-dependent antisense oligonucleotides (ASO)

Often as 2'-O-MOE/DNA chimeras that activate RNase H-mediated cleavage of target RNA.[1]

Steric-blocking ASO

Physically obstructing access to specific RNA molecules, rather than causing their degradation, e.g., splice-switching ASO.[1]

Enzyme or structural RNA inhibition

Inhibit specific RNAs or enzymes (e.g., telomerase) through binding interference.[2]

Features and Benefits

High coupling efficiency and reproducibility in solid-phase DNA synthesis
High purity (≥ 98%) and tightly controlled impurity profiles
Compatible with standard DNA/RNA phosphoramidites
Simplified purification: no special precautions needed against RNase degradation
Fast-deprotection variants available upon request

Other Notes

  • Storage: Store in a dry, inert atmosphere at -20 °C.
  • Coupling: 6 minute coupling time recommended
  • Recommended deprotection: 8 h at 55 °C in concentrated ammonia, or 10 min at 65 °C in AMA (ammonia/methylamine, 1:1 v/v)
  • Compatibility: Can be used alongside modified phosphoramidites (e.g., 2′-OMe, 2′-MOE, Locked-NA) to synthesize chimeric oligonucleotides.

Reference

[1] Hill, Alyssa C., and Jonathan Hall. "The MOE modification of RNA: Origins and widescale impact on the oligonucleotide therapeutics field." Helvetica Chimica Acta 106, no. 3 (2023): e202200169.
[2] Elayadi, Anissa N., Andrea Demieville, Edward V. Wancewicz, Brett P. Monia, and David R. Corey. "Inhibition of telomerase by 2′-O-(2-methoxyethyl) RNA oligomers: effect of length, phosphorothioate substitution and time inside cells." Nucleic Acids Research 29, no. 8 (2001): 1683-1689.

Why choose Hongene?

Trusted Partner in Nucleic Acid

Integrated Supply & Commercial Scale

With 26+ years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

Proprietary Technology & IP

Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

Rigorous Quality

We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

Manufacturing Scalability

Hongene operates 1.67 million sq. ft Oligonucleotide Manufacturing Facility, with advanced equipments including multiple OligoPilot™ and OligoProcess™ synthesizers (10-1800 mmol). 48 flexible production lines enable one-stop seamless scaling-up of API production from gram-level to tons and acheive high purity of 98%, meeting NMPA, FDA, and EMA standards.

Global Business Network

Our products and services reach over 40 countries and regions, supporting around 3,000 clients worldwide.

Global Business Distribution
40+
Countries & Regions
3000≈
Global Clients
54000+L
Annual NTP Production
58+t
Annual Amidites Production

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