DMT-dI-CE-Phosphoramidite

5'-O-(4, 4'-Dimethoxytrityl)-2'-deoxyinosine-3'-CE-Phosphoramidite
Catalog No. PD6-002
CAS No. 141684-35-7
Purity HPLC≥98.0%
Molecular Formula C40H47N6O7P
Molecular Weight 754.55
Storage Condition -20℃

DMT-dI-CE-Phosphoramidite

5'-O-(4, 4'-Dimethoxytrityl)-2'-deoxyinosine-3'-CE-Phosphoramidite
Grade
Amount/Package: 0.25g / 30mL screwed bottle-28
SKU: PD6-002-025A  |  suitable for  MerMade
Collections:  Diagnostics DNA Phosphoramidites Oligo Synthesis Phosphoramidites Phosphoramidites for Diagnostics Synthesis Blocks
Unit price:
$32.00

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Details

DMT-dI-CE-Phosphoramidite is a DNA phosphoramidite monomer used for solid-phase oligonucleotide synthesis.

It contains a deoxyinosine (dI), a 5’-dimethoxytrityl (DMT) protecting group for 5’-hydroxyl protection, and a cyanoethyl (CE) protecting group on phosphite.

dI serves as a universal base that can pair with dA, dC, or dT in DNA duplexes through wobble base-pairing.

Applications

Degenerate and universal primers

Wobble base-pairing of dI makes it particularly valuable in the design of degenerate PCR or sequencing primers.[3]

Mixed or ambiguous sequence probes

Useful in probe design for hybridization assays, mutation detection, and SNP screening where base variability must be tolerated without fully compromising binding efficiency.[4]

Features and Benefits

High purity: Manufactured to ≥98% purity with tightly controlled impurity specifications.
Compatibility: Compatible with standard phosphoramidite chemistry and most commercial synthesizers.
Excellent coupling efficiency and yield.

Other Notes

  • Diluent: Anhydrous Acetonitrile.
  • Storage: Store in a dry and inert atmosphere at -20 °C.
  • Coupling: 6 minute coupling time recommended.
  • Oxidation: 3 minute coupling time (inosine is susceptible to damage by iodine).

Reference

[1] Beaucage, S. L., and Marvin H. Caruthers. "Deoxynucleoside phosphoramidites—a new class of key intermediates for deoxypolynucleotide synthesis." Tetrahedron letters 22, no. 20 (1981): 1859-1862. [2] Caruthers, M. H., A. D. Barone, S. L. Beaucage, D. R. Dodds, E. F. Fisher, L. J. McBride, M. Matteucci, Z. Stabinsky, and J-Y. Tang. "[15] Chemical synthesis of deoxyoligonucleotides by the phosphoramidite method." In Methods in enzymology, vol. 154, pp. 287-313. Academi c Press, 1987. [3] Rossolini, Gian Maria, Stefania Cresti, Angela Ingianni, Paola Cattani, Maria Letizia Riccio, and Giuseppe Satta. "Use of deoxyinosine-containing primers vs degenerate primers for polymerase chain reaction based on ambiguous sequence information." Molecular and Cellular Probes 8, no. 2 (1994): 91-98. [4] Zhang, Jing, XiaoYan Wu, PingPing Chen, NingTing Lin, JingHua Chen, GuoNan Chen, and FengFu Fu. "Electrochemical genotyping and detection of single-nucleotide polymorphisms based on junction-probe containing 2′-deoxyinosine." Chemical Communications 46, no. 37 (2010): 6986-6988.

Specifications
  • Catalog No.
    PD6-002
  • CAS No.
    141684-35-7
  • SMILES
    O=C1C2=C(N([C@H]3C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C4=CC=C(OC)C=C4)(C5=CC=CC=C5)C6=CC=C(OC)C=C6)O3)C=N2)N=CN1
  • Molecular Formula
    C40H47N6O7P
  • Molecular Weight
    754.55
  • Appearance
    White powder
  • Purity
    HPLC≥98.0%
  • Storage Condition
    -20℃
  • Moisture Content
    K.F.≤0.20% w/w

Documentation
COA Contact Us

DMT-dI-CE-Phosphoramidite is a DNA phosphoramidite monomer used for solid-phase oligonucleotide synthesis.

It contains a deoxyinosine (dI), a 5’-dimethoxytrityl (DMT) protecting group for 5’-hydroxyl protection, and a cyanoethyl (CE) protecting group on phosphite.

dI serves as a universal base that can pair with dA, dC, or dT in DNA duplexes through wobble base-pairing.

Applications

Degenerate and universal primers

Wobble base-pairing of dI makes it particularly valuable in the design of degenerate PCR or sequencing primers.[3]

Mixed or ambiguous sequence probes

Useful in probe design for hybridization assays, mutation detection, and SNP screening where base variability must be tolerated without fully compromising binding efficiency.[4]

Features and Benefits

High purity: Manufactured to ≥98% purity with tightly controlled impurity specifications.
Compatibility: Compatible with standard phosphoramidite chemistry and most commercial synthesizers.
Excellent coupling efficiency and yield.

Other Notes

  • Diluent: Anhydrous Acetonitrile.
  • Storage: Store in a dry and inert atmosphere at -20 °C.
  • Coupling: 6 minute coupling time recommended.
  • Oxidation: 3 minute coupling time (inosine is susceptible to damage by iodine).

Reference

[1] Beaucage, S. L., and Marvin H. Caruthers. "Deoxynucleoside phosphoramidites—a new class of key intermediates for deoxypolynucleotide synthesis." Tetrahedron letters 22, no. 20 (1981): 1859-1862. [2] Caruthers, M. H., A. D. Barone, S. L. Beaucage, D. R. Dodds, E. F. Fisher, L. J. McBride, M. Matteucci, Z. Stabinsky, and J-Y. Tang. "[15] Chemical synthesis of deoxyoligonucleotides by the phosphoramidite method." In Methods in enzymology, vol. 154, pp. 287-313. Academi c Press, 1987. [3] Rossolini, Gian Maria, Stefania Cresti, Angela Ingianni, Paola Cattani, Maria Letizia Riccio, and Giuseppe Satta. "Use of deoxyinosine-containing primers vs degenerate primers for polymerase chain reaction based on ambiguous sequence information." Molecular and Cellular Probes 8, no. 2 (1994): 91-98. [4] Zhang, Jing, XiaoYan Wu, PingPing Chen, NingTing Lin, JingHua Chen, GuoNan Chen, and FengFu Fu. "Electrochemical genotyping and detection of single-nucleotide polymorphisms based on junction-probe containing 2′-deoxyinosine." Chemical Communications 46, no. 37 (2010): 6986-6988.

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With 26+ years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

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Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

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We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

Manufacturing Scalability

Hongene operates 1.67 million sq. ft Oligonucleotide Manufacturing Facility, with advanced equipments including multiple OligoPilot™ and OligoProcess™ synthesizers (10-1800 mmol). 48 flexible production lines enable one-stop seamless scaling-up of API production from gram-level to tons and acheive high purity of 98%, meeting NMPA, FDA, and EMA standards.

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