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BspQI
| Catalog No. | ON-124 |
| Purity | ＞95% by SDS-PAGE |
| Storage Condition | -20℃ |
| Source | Recombinant E.coli |
BspQI
BspQI - M (Molecular Biology) / Non-GMP / 10kU / centrifuge tube is backordered and will ship as soon as it is back in stock.
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Shipping notes
Shipping notes
Details
Details
Product Information
BspQI is a thermostable Type IIS restriction endonuclease (REase) with the recognition sequence 5'-GCTCTTCN1/N4-3'
Source: Recombinant E.coli
Concentration: 10U/μL
Unit Definition: One unit is defined as the amount of enzyme required to digest 1μg of λDNA in 1X Cut Buffer following 1 hour incubation at 50°C in a total reaction volume of 50μL.
Storage Buffer: 20mM Tris-HCl, 0.1mM EDTA, 500mM KCl, 1mM DTT, 0.1%Triton X-100, 50%(v/v) glycerol, pH7.0, 25℃.
Companion Product: 10X Cut Buffer, Cat#ON-268/ON-268-G, 500mM Tris-HCl, 1000mM NaCl, 100mM MgCl2, 10mM DTT, pH7.9, 25℃.
Key points of operation
Restriction Digest Protocol:
- On ice, combine the following reaction components in the order given:
- Incubate at 50℃, 60min.
- Heat inactivity enzyme by incubating at 80℃, 2min.
Methylation Sensitivity: Not CpG methylation-sensitive, not dam methylation-sensitive, not dcm methylation-sensitive.
Enzyme stability: BspQI half-life period at 50℃ is 2 hours in 1X Cut Buffer.
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
Specifications
Specifications
-
Catalog No.ON-124ON-124-G
-
SourceRecombinant E.coliRecombinant E.coli
-
Purity＞95% by SDS-PAGE＞95% by SDS-PAGE
-
Storage Condition-20℃-20℃
Documentation
Documentation
Product Information
BspQI is a thermostable Type IIS restriction endonuclease (REase) with the recognition sequence 5'-GCTCTTCN1/N4-3'
Source: Recombinant E.coli
Concentration: 10U/μL
Unit Definition: One unit is defined as the amount of enzyme required to digest 1μg of λDNA in 1X Cut Buffer following 1 hour incubation at 50°C in a total reaction volume of 50μL.
Storage Buffer: 20mM Tris-HCl, 0.1mM EDTA, 500mM KCl, 1mM DTT, 0.1%Triton X-100, 50%(v/v) glycerol, pH7.0, 25℃.
Companion Product: 10X Cut Buffer, Cat#ON-268/ON-268-G, 500mM Tris-HCl, 1000mM NaCl, 100mM MgCl2, 10mM DTT, pH7.9, 25℃.
Key points of operation
Restriction Digest Protocol:
- On ice, combine the following reaction components in the order given:
- Incubate at 50℃, 60min.
- Heat inactivity enzyme by incubating at 80℃, 2min.
Methylation Sensitivity: Not CpG methylation-sensitive, not dam methylation-sensitive, not dcm methylation-sensitive.
Enzyme stability: BspQI half-life period at 50℃ is 2 hours in 1X Cut Buffer.
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
-
Catalog No.ON-124ON-124-G
-
SourceRecombinant E.coliRecombinant E.coli
-
Purity＞95% by SDS-PAGE＞95% by SDS-PAGE
-
Storage Condition-20℃-20℃
Why choose Hongene?
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Integrated Supply & Commercial Scale
With nearly 30 years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.
Proprietary Technology & IP
Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.
Rigorous Quality
We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.
Manufacturing Scalability
Hongene operates 1.67 million sq. ft Oligonucleotide Manufacturing Facility, with advanced equipments including multiple OligoPilot™ and OligoProcess™ synthesizers (10-1800 mmol). 48 flexible production lines enable one-stop seamless scaling-up of API production from gram-level to tons and acheive high purity of 98%, meeting NMPA, FDA, and EMA standards.
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