This document is the 2024 mRNA Product Catalog published by Hongene Biotech, providing a comprehensive introduction to its full range of raw materials, enzymes, and kits for in vitro transcription (IVT) synthesis of mRNA.
The key contents are as follows:
- Integrated Solution: The catalog begins with a schematic diagram of the entire mRNA synthesis process, clearly outlining each step from plasmid linearization to the final mRNA Active Pharmaceutical Ingredient (API), with corresponding Hongene products indicated for each stage.
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Core Products & Services:
- Custom mRNA Synthesis Service: Offers GMP-standard custom mRNA services, promising high integrity (>90%), high capping efficiency (Cap1 structure, up to 99.8%), long Poly(A) tail (100 nt), and very low levels of dsRNA (as low as 0.002%) and endotoxins.
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Enzymes: Supplies high-quality enzymes for the entire mRNA synthesis workflow, including:
- Template Preparation: BspQI (BSA-free) restriction enzyme.
- Transcription: T7 and SP6 RNA polymerases and their high-yield kits.
- Accessory Enzymes: Inorganic Pyrophosphatase (increases yield), DNase I (template removal), RNase Inhibitor (prevents degradation).
- Capping & Tailing: Vaccinia Capping Enzyme, mRNA Cap 2'-O-Methyltransferase (for Cap1 structure synthesis), Poly(A) Polymerase.
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Substrates & Raw Materials:
- Nucleotides (NTPs): Offers a variety of natural and modified NTPs (e.g., N1-Methyl-pseudoUTP, 5-Me-CTP), available in both Sodium salt and Tris salt solutions.
- Cap Analogs: Provides various cap analogs including GpppG, m7GpppG (Cap 0), m7GpppA (Cap 0), and the GAG Trimer.
- CDMO Services: The company highlights its comprehensive Contract Development and Manufacturing Organization (CDMO) capabilities, from raw materials to final drug product.
- Quality Assurance: The catalog details product information such as unit definitions, concentrations, storage buffers, and reaction buffers for each item, reflecting stringent quality control.
(For the complete product list, catalog numbers, detailed technical specifications, and pricing, please download the full PDF catalog.)