Nuclease-free H2O
to 50μL
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Taq DNA Ligase
| Catalog No. | ON-308 |
| Storage Condition | -20℃ |
| Source | Recombinant E.coli |
| Purity | ＞95% by SDS-PAGE |
Taq DNA Ligase
Catalog No: ON-308
Taq DNA Ligase - M (Molecular Biology) is backordered and will ship as soon as it is back in stock.
SKU:
ON-308
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Shipping notes
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Excerpt from Shipping Policy
Details
Details
Product Information
Product description: Taq DNA ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD+ as a cofactor.
Source: Recombinant E.coli
Concentration: 40U/μL
Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1μg of BstEII-digested λDNA in a total reaction volume of 50μL in 15minutes at 45℃.
Storage Buffer: 10mM Tris-HCl (pH 7.5, 25℃), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1% Triton X-100 and 50% (v/v)Glyercol.
Companion Product: 10X Taq DNA Ligase Reaction Buffer, Cat#ON-072, 200mM Tris-HCl pH 7.6, 25℃, 250mM KAC, 100mM Mg(AC)2, 10mM NAD+,0.1% Triton X-100.
Key points of operation
Note: The ligation reaction requires NAD+ as a cofactor. NAD+ is supplied in the 10×Taq DNA Ligase Reaction Buffer, the buffer should be stored at -70℃ to extend the half life of the NAD+ cofactor. Taq DNA Ligase is active at 45-65℃
Recommended Protocol:
- Add the following reagents in a PCR tube:
Component
Amount
DNA
up to 1μg of DNA
10X Taq DNA Ligase Reaction Buffer
5μL
Taq DNA Ligase
2μL (80 units)
- Incubate at 45℃ for 15 min.
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease, and Nickase activities.
Physical Purity: ＞95% by SDS-PAGE.
Reference
1. Barany F. Proc Nat Acad Sci USA. 88, 189–193 (1991).
2. Gail Lauer, Edwin A. Rudd, Dianel L. Mckay, et al. Journal Of Bacteriology. 173, 5047-5053(1991).
Specifications
Specifications
-
Catalog No.ON-308
-
SourceRecombinant E.coli
-
Purity＞95% by SDS-PAGE
-
Storage Condition-20℃
Documentation
Documentation
Product Information
Product description: Taq DNA ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD+ as a cofactor.
Source: Recombinant E.coli
Concentration: 40U/μL
Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1μg of BstEII-digested λDNA in a total reaction volume of 50μL in 15minutes at 45℃.
Storage Buffer: 10mM Tris-HCl (pH 7.5, 25℃), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1% Triton X-100 and 50% (v/v)Glyercol.
Companion Product: 10X Taq DNA Ligase Reaction Buffer, Cat#ON-072, 200mM Tris-HCl pH 7.6, 25℃, 250mM KAC, 100mM Mg(AC)2, 10mM NAD+,0.1% Triton X-100.
Key points of operation
Note: The ligation reaction requires NAD+ as a cofactor. NAD+ is supplied in the 10×Taq DNA Ligase Reaction Buffer, the buffer should be stored at -70℃ to extend the half life of the NAD+ cofactor. Taq DNA Ligase is active at 45-65℃
Recommended Protocol:
- Add the following reagents in a PCR tube:
Component
Amount
Nuclease-free H2O
to 50μL
DNA
up to 1μg of DNA
10X Taq DNA Ligase Reaction Buffer
5μL
Taq DNA Ligase
2μL (80 units)
- Incubate at 45℃ for 15 min.
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease, and Nickase activities.
Physical Purity: ＞95% by SDS-PAGE.
Reference
1. Barany F. Proc Nat Acad Sci USA. 88, 189–193 (1991).
2. Gail Lauer, Edwin A. Rudd, Dianel L. Mckay, et al. Journal Of Bacteriology. 173, 5047-5053(1991).
-
Catalog No.ON-308
-
SourceRecombinant E.coli
-
Purity＞95% by SDS-PAGE
-
Storage Condition-20℃
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