Taq DNA polymerase

Catalog No. ON-007
Storage Condition -20℃
Source Recombinant E.coli
Purity ＞95% by SDS-PAGE

Taq DNA polymerase

Grade
SKU: ON-007
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Details

Product Information

Product description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5'→ 3' polymerase activity and 5'→ 3' exonuclease activity. The PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine(A) to the 3' end. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 94kD.

Source: Recombinant E.coli

Concentration: 5U/μL

Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmols of total dNTPs into acid-insoluble DNA in 30min at 74℃.

Storage Buffer: 20mM Tris-HCl (pH7.9, 25℃), 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 0.5% NP40, 1mM DTT, 50%(v/v) Glycerol.

Companion Products: 10X Reaction Buffer, Cat#ON-012, 100mM Tris-HCl, 500mM KCl, pH9.0, 25℃; 100mM MgCl2, Cat#ON-013.

Key points of operation

  1. Recommended reaction mixture:
Component
Final Volume
Final Conc.
10X Reaction Buffer
5μL
1X
dNTP Mix, 10mM each
1
0.2mM each
25mM MgCl2
3
1.5mM
upstream primer
X μL
0.1-1.0μM
downstream primer
Y μL
0.1-1.0μM
Taq (5U/μL)
0.25μL
1.25U
Template DNA
Z μL
< 0.5μg/50μL
Nuclease-free H2O
to 50μL

  1. Recommended reaction conditions:
Cycle
Time
Temp ℃
Initial Denaturation
5μL
2min
95
Denaturation
1
0.5-1min
94
Annealing
3
0.5-1min
Tm-5
Extension
X μL
1min/kb
72
Final Extension
Y μL
5min
72

    Optimal enzyme concentration: 0.5U-5U per 50μL volume (50μL volume: 1.25U recommended)

    Optimal MgCl2 concentration: 1.5mM (1~5mM)

Security Information

Storage Conditions: -20℃

Quality Assurance: Free of Endonuclease, Exonuclease, Nickase and RNase activities. Free of 16s rDNA.

Physical Purity: ＞95% by SDS-PAGE.

Reference

1. Lawyer FC,et al. PCR Methods Appl. 2(4):275-87(1993).

2. Chien A, Edgar DB, Trela JM.J. Bacteriol 127(3):1550-7(1976).

Specifications
  • Catalog No.
    ON-007
  • Source
    Recombinant E.coli
  • Purity
    ＞95% by SDS-PAGE
  • Storage Condition
    -20℃

Documentation
COA Contact Us

Product Information

Product description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5'→ 3' polymerase activity and 5'→ 3' exonuclease activity. The PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine(A) to the 3' end. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 94kD.

Source: Recombinant E.coli

Concentration: 5U/μL

Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmols of total dNTPs into acid-insoluble DNA in 30min at 74℃.

Storage Buffer: 20mM Tris-HCl (pH7.9, 25℃), 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 0.5% NP40, 1mM DTT, 50%(v/v) Glycerol.

Companion Products: 10X Reaction Buffer, Cat#ON-012, 100mM Tris-HCl, 500mM KCl, pH9.0, 25℃; 100mM MgCl2, Cat#ON-013.

Key points of operation

  1. Recommended reaction mixture:
Component
Final Volume
Final Conc.
10X Reaction Buffer
5μL
1X
dNTP Mix, 10mM each
1
0.2mM each
25mM MgCl2
3
1.5mM
upstream primer
X μL
0.1-1.0μM
downstream primer
Y μL
0.1-1.0μM
Taq (5U/μL)
0.25μL
1.25U
Template DNA
Z μL
< 0.5μg/50μL
Nuclease-free H2O
to 50μL

  1. Recommended reaction conditions:
Cycle
Time
Temp ℃
Initial Denaturation
5μL
2min
95
Denaturation
1
0.5-1min
94
Annealing
3
0.5-1min
Tm-5
Extension
X μL
1min/kb
72
Final Extension
Y μL
5min
72

    Optimal enzyme concentration: 0.5U-5U per 50μL volume (50μL volume: 1.25U recommended)

    Optimal MgCl2 concentration: 1.5mM (1~5mM)

Security Information

Storage Conditions: -20℃

Quality Assurance: Free of Endonuclease, Exonuclease, Nickase and RNase activities. Free of 16s rDNA.

Physical Purity: ＞95% by SDS-PAGE.

Reference

1. Lawyer FC,et al. PCR Methods Appl. 2(4):275-87(1993).

2. Chien A, Edgar DB, Trela JM.J. Bacteriol 127(3):1550-7(1976).

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