Taq DNA polymerase

Catalog No. ON-007
Purity ＞95% by SDS-PAGE
Storage Condition -20℃
Source Recombinant E.coli

Taq DNA polymerase

Grade
SKU: ON-007
Collections:  Biochemical Reagents Diagnostics Enzyme & Related Buffer for Diagnostics Enzymes Enzymes & Buffers Enzymes for Diagnostics Other Raw Materials PCR
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Details

Product Information

Product description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5'→ 3' polymerase activity and 5'→ 3' exonuclease activity. The PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine(A) to the 3' end. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 94kD.

Source: Recombinant E.coli

Concentration: 5U/μL

Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmols of total dNTPs into acid-insoluble DNA in 30min at 74℃.

Storage Buffer: 20mM Tris-HCl (pH7.9, 25℃), 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 0.5% NP40, 1mM DTT, 50%(v/v) Glycerol.

Companion Products: 10X Reaction Buffer, Cat#ON-012, 100mM Tris-HCl, 500mM KCl, pH9.0, 25℃; 100mM MgCl2, Cat#ON-013.

Key points of operation

  1. Recommended reaction mixture:
Component
Final Volume
Final Conc.
10X Reaction Buffer
5μL
1X
dNTP Mix, 10mM each
1
0.2mM each
25mM MgCl2
3
1.5mM
upstream primer
X μL
0.1-1.0μM
downstream primer
Y μL
0.1-1.0μM
Taq (5U/μL)
0.25μL
1.25U
Template DNA
Z μL
< 0.5μg/50μL
Nuclease-free H2O
to 50μL

  1. Recommended reaction conditions:
Cycle
Time
Temp ℃
Initial Denaturation
5μL
2min
95
Denaturation
1
0.5-1min
94
Annealing
3
0.5-1min
Tm-5
Extension
X μL
1min/kb
72
Final Extension
Y μL
5min
72

    Optimal enzyme concentration: 0.5U-5U per 50μL volume (50μL volume: 1.25U recommended)

    Optimal MgCl2 concentration: 1.5mM (1~5mM)

Security Information

Storage Conditions: -20℃

Quality Assurance: Free of Endonuclease, Exonuclease, Nickase and RNase activities. Free of 16s rDNA.

Physical Purity: ＞95% by SDS-PAGE.

Reference

1. Lawyer FC,et al. PCR Methods Appl. 2(4):275-87(1993).

2. Chien A, Edgar DB, Trela JM.J. Bacteriol 127(3):1550-7(1976).

Specifications
  • Catalog No.
    ON-007
  • Source
    Recombinant E.coli
  • Purity
    ＞95% by SDS-PAGE
  • Storage Condition
    -20℃

Documentation
COA Contact Us

Product Information

Product description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5'→ 3' polymerase activity and 5'→ 3' exonuclease activity. The PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine(A) to the 3' end. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 94kD.

Source: Recombinant E.coli

Concentration: 5U/μL

Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmols of total dNTPs into acid-insoluble DNA in 30min at 74℃.

Storage Buffer: 20mM Tris-HCl (pH7.9, 25℃), 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 0.5% NP40, 1mM DTT, 50%(v/v) Glycerol.

Companion Products: 10X Reaction Buffer, Cat#ON-012, 100mM Tris-HCl, 500mM KCl, pH9.0, 25℃; 100mM MgCl2, Cat#ON-013.

Key points of operation

  1. Recommended reaction mixture:
Component
Final Volume
Final Conc.
10X Reaction Buffer
5μL
1X
dNTP Mix, 10mM each
1
0.2mM each
25mM MgCl2
3
1.5mM
upstream primer
X μL
0.1-1.0μM
downstream primer
Y μL
0.1-1.0μM
Taq (5U/μL)
0.25μL
1.25U
Template DNA
Z μL
< 0.5μg/50μL
Nuclease-free H2O
to 50μL

  1. Recommended reaction conditions:
Cycle
Time
Temp ℃
Initial Denaturation
5μL
2min
95
Denaturation
1
0.5-1min
94
Annealing
3
0.5-1min
Tm-5
Extension
X μL
1min/kb
72
Final Extension
Y μL
5min
72

    Optimal enzyme concentration: 0.5U-5U per 50μL volume (50μL volume: 1.25U recommended)

    Optimal MgCl2 concentration: 1.5mM (1~5mM)

Security Information

Storage Conditions: -20℃

Quality Assurance: Free of Endonuclease, Exonuclease, Nickase and RNase activities. Free of 16s rDNA.

Physical Purity: ＞95% by SDS-PAGE.

Reference

1. Lawyer FC,et al. PCR Methods Appl. 2(4):275-87(1993).

2. Chien A, Edgar DB, Trela JM.J. Bacteriol 127(3):1550-7(1976).

Why choose Hongene?

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With nearly 30 years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

Proprietary Technology & IP

Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

Rigorous Quality

We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

Manufacturing Scalability

Hongene operates a 1.67-million-square-foot oligonucleotide manufacturing facility featuring advanced equipment, including multiple OligoPilot™ and OligoProcess™ synthesizers ranging from 10 to 1,800 mmol. Its 48 flexible production lines enable seamless, one-stop scale-up of API production from gram-level quantities to metric tons, achieving purity of ≥98% while supporting compliance with NMPA, FDA, and EMA regulatory requirements.

Global Business Network

Our products and services reach over 40 countries and regions, supporting around 3,000 clients worldwide.

Global Business Distribution
40+
Countries & Regions
≈3000
Global Clients
54000L+
Annual NTP Production
58t+
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