10X Reaction Buffer
5μL
1X
Taq DNA polymerase
| Catalog No. | ON-007 |
| Purity | ＞95% by SDS-PAGE |
| Storage Condition | -20℃ |
| Source | Recombinant E.coli |
Taq DNA polymerase
Catalog No: ON-007
Taq DNA polymerase - M (Molecular Biology) is backordered and will ship as soon as it is back in stock.
SKU:
ON-007
| suitable for
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Shipping notes
Shipping notes
Products on this site:
Currently ship to United States & Canada.
For shipping worldwide, please contact us.
Processment: within 2 business days, if available.
US: 2-8 days (from CA by FedEx).
Canada: 5-10 days (excludes customs delays).
Details
Details
Product Information
Product description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5'→ 3' polymerase activity and 5'→ 3' exonuclease activity. The PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine(A) to the 3' end. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 94kD.
Source: Recombinant E.coli
Concentration: 5U/μL
Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmols of total dNTPs into acid-insoluble DNA in 30min at 74℃.
Storage Buffer: 20mM Tris-HCl (pH7.9, 25℃), 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 0.5% NP40, 1mM DTT, 50%(v/v) Glycerol.
Companion Products: 10X Reaction Buffer, Cat#ON-012, 100mM Tris-HCl, 500mM KCl, pH9.0, 25℃; 100mM MgCl2, Cat#ON-013.
Key points of operation
- Recommended reaction mixture:
Component
Final Volume
Final Conc.
dNTP Mix, 10mM each
1
0.2mM each
25mM MgCl2
3
1.5mM
upstream primer
X μL
0.1-1.0μM
downstream primer
Y μL
0.1-1.0μM
Taq (5U/μL)
0.25μL
1.25U
Template DNA
Z μL
< 0.5μg/50μL
Nuclease-free H2O
to 50μL
- Recommended reaction conditions:
Cycle
Time
Temp ℃
Initial Denaturation
5μL
2min
95
Denaturation
1
0.5-1min
94
Annealing
3
0.5-1min
Tm-5
Extension
X μL
1min/kb
72
Final Extension
Y μL
5min
72
-
Optimal enzyme concentration: 0.5U-5U per 50μL volume (50μL volume: 1.25U recommended)
Optimal MgCl2 concentration: 1.5mM (1~5mM)
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of Endonuclease, Exonuclease, Nickase and RNase activities. Free of 16s rDNA.
Physical Purity: ＞95% by SDS-PAGE.
Reference
1. Lawyer FC,et al. PCR Methods Appl. 2(4):275-87(1993).
2. Chien A, Edgar DB, Trela JM.J. Bacteriol 127(3):1550-7(1976).
Specifications
Specifications
-
Catalog No.ON-007
-
SourceRecombinant E.coli
-
Purity＞95% by SDS-PAGE
-
Storage Condition-20℃
Documentation
Documentation
Product Information
Product description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5'→ 3' polymerase activity and 5'→ 3' exonuclease activity. The PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine(A) to the 3' end. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 94kD.
Source: Recombinant E.coli
Concentration: 5U/μL
Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmols of total dNTPs into acid-insoluble DNA in 30min at 74℃.
Storage Buffer: 20mM Tris-HCl (pH7.9, 25℃), 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 0.5% NP40, 1mM DTT, 50%(v/v) Glycerol.
Companion Products: 10X Reaction Buffer, Cat#ON-012, 100mM Tris-HCl, 500mM KCl, pH9.0, 25℃; 100mM MgCl2, Cat#ON-013.
Key points of operation
- Recommended reaction mixture:
Component
Final Volume
Final Conc.
10X Reaction Buffer
5μL
1X
dNTP Mix, 10mM each
1
0.2mM each
25mM MgCl2
3
1.5mM
upstream primer
X μL
0.1-1.0μM
downstream primer
Y μL
0.1-1.0μM
Taq (5U/μL)
0.25μL
1.25U
Template DNA
Z μL
< 0.5μg/50μL
Nuclease-free H2O
to 50μL
- Recommended reaction conditions:
Cycle
Time
Temp ℃
Initial Denaturation
5μL
2min
95
Denaturation
1
0.5-1min
94
Annealing
3
0.5-1min
Tm-5
Extension
X μL
1min/kb
72
Final Extension
Y μL
5min
72
-
Optimal enzyme concentration: 0.5U-5U per 50μL volume (50μL volume: 1.25U recommended)
Optimal MgCl2 concentration: 1.5mM (1~5mM)
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of Endonuclease, Exonuclease, Nickase and RNase activities. Free of 16s rDNA.
Physical Purity: ＞95% by SDS-PAGE.
Reference
1. Lawyer FC,et al. PCR Methods Appl. 2(4):275-87(1993).
2. Chien A, Edgar DB, Trela JM.J. Bacteriol 127(3):1550-7(1976).
-
Catalog No.ON-007
-
SourceRecombinant E.coli
-
Purity＞95% by SDS-PAGE
-
Storage Condition-20℃
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