What are Dyes & Quenchers?
Dyes (fluorophores) are molecules that absorb light at specific wavelengths and re-emit it at longer wavelengths, generating detectable fluorescence. Quenchers are molecules that absorb this excitation energy from a fluorophore and dissipate it either as heat (dark quenchers) or as light at a different wavelength (fluorescent quenchers). Together, they form the cornerstone of fluorescence-based detection technologies.
Our comprehensive product line includes:
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Classic Dyes: FAM, TAMRA, HEX, TET
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Cyanine Dyes: Cyanine3 and Cyanine5, providing emissions across the visible spectrum
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Specialty Labels: Biotin for affinity purification and detection
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Dark Quenchers: Non-fluorescent quenchers (HDQ in Hongene) that minimize background noise by converting energy to heat
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Fluorescent Quenchers: Such as TAMRA, suitable for certain FRET applications
Synthesis and Oligo Integration
Our dyes and quenchers are primarily supplied as phosphoramidites or solid supports, enabling seamless integration during automated oligonucleotide synthesis using standard protocols.
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Phosphoramidite Chemistry: Dye and quencher phosphoramidites serve as building blocks that can be directly incorporated at the 5' end, 3' end, or at any internal position of an oligonucleotide during synthesis. This ensures precise stoichiometry and site-specific labeling.
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Post-Synthetic Conjugation: For labels like biotin or certain reactive esters, we provide modifiers for efficient conjugation to synthesized oligos or other biomolecules after synthesis.
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Strict QC: Every batch undergoes rigorous purification (HPLC) to guarantee specified performance metrics.
