Dyes & Quenchers

(21 products)

What are Dyes & Quenchers?
Dyes (fluorophores) are molecules that absorb light at specific wavelengths and re-emit it at longer wavelengths, generating detectable fluorescence. Quenchers are molecules that absorb this excitation energy from a fluorophore and dissipate it either as heat (dark quenchers) or as light at a different wavelength (fluorescent quenchers). Together, they form the cornerstone of fluorescence-based detection technologies.

Our comprehensive product line includes:

  • Classic Dyes: FAM, TAMRA, HEX, TET
  • Cyanine Dyes: Cyanine3 and Cyanine5, providing emissions across the visible spectrum
  • Specialty Labels: Biotin for affinity purification and detection
  • Dark Quenchers: Non-fluorescent quenchers (HDQ in Hongene) that minimize background noise by converting energy to heat
  • Fluorescent Quenchers: Such as TAMRA, suitable for certain FRET applications

Synthesis and Oligo Integration
Our dyes and quenchers are primarily supplied as phosphoramidites or solid supports, enabling seamless integration during automated oligonucleotide synthesis using standard protocols.

  • Phosphoramidite Chemistry: Dye and quencher phosphoramidites serve as building blocks that can be directly incorporated at the 5' end, 3' end, or at any internal position of an oligonucleotide during synthesis. This ensures precise stoichiometry and site-specific labeling.
  • Post-Synthetic Conjugation: For labels like biotin or certain reactive esters, we provide modifiers for efficient conjugation to synthesized oligos or other biomolecules after synthesis.
  • Strict QC: Every batch undergoes rigorous purification (HPLC) to guarantee specified performance metrics.

Our Advantages

By combining expertise in organic synthesis and biological applications, we provide the foundational tools that bring clarity and discovery to your work:

Exceptional Spectral Consistency

Our rigorous manufacturing and quality control processes ensure that every batch delivers identical spectral properties, enabling reliable and reproducible experimental results.

Enhanced Brightness and Stability

Our fluorophores are engineered to provide strong fluorescence intensity and exceptional resistance to photobleaching, ensuring sustained signal integrity throughout your experiments.

Superior Quenching Performance

Our dark quenchers demonstrate excellent energy absorption capabilities, effectively minimizing background fluorescence and significantly enhancing signal-to-noise ratios in detection assays.

Scalable Production Capabilities

Our manufacturing processes support projects at any scale, from research-grade quantities to commercial-scale production, all maintained under stringent quality standards.

Applications of Our Dyes & Quenchers

Our products are essential components in a wide array of scientific and diagnostic applications:

Genetic Analysis & Molecular Diagnostics

  1. Real-Time PCR: Probe-based assays utilizing our FAM/BHQ-1 pairs and molecular beacons for precise, quantitative pathogen detection and gene expression analysis.
  2. DNA Sequencing: Fluorescently labeled dNTPs and primers for high-throughput sequencing and genome analysis.
  3. Microarrays: Oligonucleotides labeled with cyanine dyes or biotin for highly multiplexed expression or genotyping arrays.

Biomedical Imaging & Microscopy

  1. Fluorescence In Situ Hybridization: Labeled probes for high-resolution chromosomal and cellular imaging.
  2. Live-Cell Imaging: Photostable dyes for tracking dynamic cellular processes over time.
  3. Cellular Analysis: Fluorescent dyes for various cellular imaging applications with good photostability.

Therapeutic & Diagnostic Applications

  1. Antisense Oligonucleotides & siRNA: Dye-labeled therapeutic oligos for tracking cellular uptake and distribution.
  2. Targeted Delivery Systems: Antibody-dye conjugates for imaging and delivering therapeutic agents to specific cells.

Drug Discovery & Development

  1. High-Throughput Screening: Fluorescent probes and quenched substrates to identify enzyme inhibitors or receptor modulators.
  2. Flow Cytometry: Antibodies conjugated to our spectrum of dyes enable complex immunophenotyping panels.

Biosensor Development & Assays

  1. FRET-Based Sensors: Optimized dye-quencher pairs for developing sensors that detect enzymatic activity, ion concentration, or biomolecular interactions.
  2. Lateral Flow Assays: Antibodies conjugated to our dyes or biotin provide visible test lines for rapid point-of-care diagnostics.
  3. Homogeneous Assay Formats: Antibody and protein conjugates for highly sensitive detection platforms.
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