BspQI

Catalog No. ON-124
Purity ＞95% by SDS-PAGE
Storage Condition -20℃
Source Recombinant E.coli

BspQI

Grade
GMP level
Amount/Package: 10kU / centrifuge tube
SKU: ON-124-KU010-P
Collections:  Biochemical Reagents Enzyme & Related Buffer for mRNA Enzymes Enzymes & Buffers Enzymes for mRNA Synthesis mRNA Other Raw Materials Restriction Endonucleases
Unit price:
$595.00

Price

$595.00
Shipping calculated at checkout.
This variant is unavailable. Contact us if you have particular requirements.
Checkout

This variant only support customization. Click the Inquiry button below to submit your custom request to us.

Price/Order Inquiry

Shipping notes
Products on this site:
Currently ship to United States & Canada.
For shipping worldwide, please contact us.
Processment: within 2 business days, if available.
US: 2-8 days (from CA by FedEx).
Canada: 5-10 days (excludes customs delays).

Details

Product Information

BspQI is a thermostable Type IIS restriction endonuclease (REase) with the recognition sequence 5'-GCTCTTCN1/N4-3'

5'
...
G
C
T
C
T
T
C
(N)1↓
...
3'
3'
...
C
G
A
G
A
A
G
(N)4↑
...
5'

Source: Recombinant E.coli
Concentration: 10U/μL
Unit Definition: One unit is defined as the amount of enzyme required to digest 1μg of λDNA in 1X Cut Buffer following 1 hour incubation at 50°C in a total reaction volume of 50μL.
Storage Buffer: 20mM Tris-HCl, 0.1mM EDTA, 500mM KCl, 1mM DTT, 0.1%Triton X-100, 50%(v/v) glycerol, pH7.0, 25℃.
Companion Product: 10X Cut Buffer, Cat#ON-268/ON-268-G, 500mM Tris-HCl, 1000mM NaCl, 100mM MgCl2, 10mM DTT, pH7.9, 25℃.

Key points of operation

Restriction Digest Protocol:

  1. On ice, combine the following reaction components in the order given:
Component
Amount
DNA
1μg
10X Cut Buffer
5μL
BspQI
10 units
Total Reaction Volume
50μL
  1. Incubate at 50℃, 60min.
  2. Heat inactivity enzyme by incubating at 80℃, 2min.

Methylation Sensitivity: Not CpG methylation-sensitive, not dam methylation-sensitive, not dcm methylation-sensitive.
Enzyme stability: BspQI half-life period at 50℃ is 2 hours in 1X Cut Buffer.

Security Information

Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.

Specifications
  • Catalog No.
    ON-124
  • Source
    Recombinant E.coli
  • Purity
    ＞95% by SDS-PAGE
  • Storage Condition
    -20℃

Documentation
COA Contact Us

Product Information

BspQI is a thermostable Type IIS restriction endonuclease (REase) with the recognition sequence 5'-GCTCTTCN1/N4-3'

5'
...
G
C
T
C
T
T
C
(N)1↓
...
3'
3'
...
C
G
A
G
A
A
G
(N)4↑
...
5'

Source: Recombinant E.coli
Concentration: 10U/μL
Unit Definition: One unit is defined as the amount of enzyme required to digest 1μg of λDNA in 1X Cut Buffer following 1 hour incubation at 50°C in a total reaction volume of 50μL.
Storage Buffer: 20mM Tris-HCl, 0.1mM EDTA, 500mM KCl, 1mM DTT, 0.1%Triton X-100, 50%(v/v) glycerol, pH7.0, 25℃.
Companion Product: 10X Cut Buffer, Cat#ON-268/ON-268-G, 500mM Tris-HCl, 1000mM NaCl, 100mM MgCl2, 10mM DTT, pH7.9, 25℃.

Key points of operation

Restriction Digest Protocol:

  1. On ice, combine the following reaction components in the order given:
Component
Amount
DNA
1μg
10X Cut Buffer
5μL
BspQI
10 units
Total Reaction Volume
50μL
  1. Incubate at 50℃, 60min.
  2. Heat inactivity enzyme by incubating at 80℃, 2min.

Methylation Sensitivity: Not CpG methylation-sensitive, not dam methylation-sensitive, not dcm methylation-sensitive.
Enzyme stability: BspQI half-life period at 50℃ is 2 hours in 1X Cut Buffer.

Security Information

Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.

Why choose Hongene?

Trusted Partner in Nucleic Acid

Integrated Supply & Commercial Scale

With 26+ years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

Proprietary Technology & IP

Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

Rigorous Quality

We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

Manufacturing Scalability

Hongene operates 1.67 million sq. ft Oligonucleotide Manufacturing Facility, with advanced equipments including multiple OligoPilot™ and OligoProcess™ synthesizers (10-1800 mmol). 48 flexible production lines enable one-stop seamless scaling-up of API production from gram-level to tons and acheive high purity of 98%, meeting NMPA, FDA, and EMA standards.

Global Business Network

Our products and services reach over 40 countries and regions, supporting around 3,000 clients worldwide.

Global Business Distribution
40+
Countries & Regions
3000≈
Global Clients
54000+L
Annual NTP Production
58+t
Annual Amidites Production

Ask us anything!

Back to top

My Wishlist