ddH2O
X μL
RNase III, E.coli
| Catalog No. | ON-024 |
| Storage Condition | -70℃ |
| Source | Recombinant E.coli |
| Purity | ＞90% by SDS-PAGE |
RNase III, E.coli
Catalog No: ON-024
RNase III, E.coli - M (Molecular Biology) / 0.5kU / centrifuge tube is backordered and will ship as soon as it is back in stock.
SKU:
ON-024-U500-P
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Shipping notes
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Excerpt from Shipping Policy
Related Products
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Catalog No.: ON-004-C050
Purity: ＞95% by SDS-PAGE
Source: Recombinant E.coli
From$22.50From $0.00Unit price /Unavailable -
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Details
Details
Product Information
Product description: RNase III, E.coli is a homodimeric, divalent metal ion dependent nuclease. RNase III cleaves long double-stranded RNA (dsRNA) into short 12-30 base dsRNAs containing a 5'-PO4, 3'-OH, and a dinucleotide 3' overhang.
Source: Recombinant E.coli
Concentration: 0.5U/μL
Unit Definition: One unit is the amount of enzyme required to digest 1μg of dsRNA to siRNA in 20 minutes at 37℃ in a total reaction volume of 50μL.
Storage Buffer: 500mM NaCl, 20mM Tris-HCl (pH 8.0, 25℃), 0.5mM EDTA, 0.5mM DTT, 50% Glyercol.
Companion Product:
10X RNaseIII Reaction Buffer, Cat#ON-078, 500mM Tris-HCl (pH7.5, 25℃), 500mM NaCl, 10mM DTT
10X MnCl2, Cat#ON-079, 200mM MnCl2
10X EDTA, Cat#ON-080, 500mM EDTA
Key points of operation
Recommended protocol:
- In ice, combine the following, in order:
Component
Amount
10X RNase III Reaction Buffer
10μL
dsRNA
X μL (10μg)
RNase III
10-20 units
10X MnCl2
10μL
Total Volume
100μL
- Mix and incubate for 20 minutes at 37℃.
- Add 10μL 10X EDTA to stop the reaction.
Security Information
Storage Conditions:
Long term (Infrequent use): -70℃
Daily/Weekly use: -20℃*
Do not store in a frost-free freezer.
Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.
* RNase III stores at -20℃ may result in the formation of haze. If this happens, keep the tube at room temperature until tube temperature is up to 4~10℃. Then mix gently to clarify the solution. Haze should disappear. And there is negligible loss of activity.
Quality Assurance: Free of Endonuclease, Exonuclease, and Nickase activities. Pass functional testing
Physical Purity: ＞90% by SDS-PAGE.
Reference
1. Hugh D. Robertson, Robent E. Webster, and Norton D. Zinder. The Journal of Biological Chemistry. 243, 82-91(1968).
2. Neelam Srivastava and Rai Ajit K Srivastava. Biochemistry and Molecular Biology International. 39, 171-180(1996).
Specifications
Specifications
-
Catalog No.ON-024
-
SourceRecombinant E.coli
-
Purity＞90% by SDS-PAGE
-
Storage Condition-70℃
Documentation
Documentation
Product Information
Product description: RNase III, E.coli is a homodimeric, divalent metal ion dependent nuclease. RNase III cleaves long double-stranded RNA (dsRNA) into short 12-30 base dsRNAs containing a 5'-PO4, 3'-OH, and a dinucleotide 3' overhang.
Source: Recombinant E.coli
Concentration: 0.5U/μL
Unit Definition: One unit is the amount of enzyme required to digest 1μg of dsRNA to siRNA in 20 minutes at 37℃ in a total reaction volume of 50μL.
Storage Buffer: 500mM NaCl, 20mM Tris-HCl (pH 8.0, 25℃), 0.5mM EDTA, 0.5mM DTT, 50% Glyercol.
Companion Product:
10X RNaseIII Reaction Buffer, Cat#ON-078, 500mM Tris-HCl (pH7.5, 25℃), 500mM NaCl, 10mM DTT
10X MnCl2, Cat#ON-079, 200mM MnCl2
10X EDTA, Cat#ON-080, 500mM EDTA
Key points of operation
Recommended protocol:
- In ice, combine the following, in order:
Component
Amount
ddH2O
X μL
10X RNase III Reaction Buffer
10μL
dsRNA
X μL (10μg)
RNase III
10-20 units
10X MnCl2
10μL
Total Volume
100μL
- Mix and incubate for 20 minutes at 37℃.
- Add 10μL 10X EDTA to stop the reaction.
Security Information
Storage Conditions:
Long term (Infrequent use): -70℃
Daily/Weekly use: -20℃*
Do not store in a frost-free freezer.
Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.
* RNase III stores at -20℃ may result in the formation of haze. If this happens, keep the tube at room temperature until tube temperature is up to 4~10℃. Then mix gently to clarify the solution. Haze should disappear. And there is negligible loss of activity.
Quality Assurance: Free of Endonuclease, Exonuclease, and Nickase activities. Pass functional testing
Physical Purity: ＞90% by SDS-PAGE.
Reference
1. Hugh D. Robertson, Robent E. Webster, and Norton D. Zinder. The Journal of Biological Chemistry. 243, 82-91(1968).
2. Neelam Srivastava and Rai Ajit K Srivastava. Biochemistry and Molecular Biology International. 39, 171-180(1996).
-
Catalog No.ON-024
-
SourceRecombinant E.coli
-
Purity＞90% by SDS-PAGE
-
Storage Condition-70℃
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