5-Me-CTP, 100mM solution

5-methyl-cytidine-5'-triphosphate, sodium salt solution
Catalog No. R3-029
CAS No. 327174-86-7 (free acid)
Purity HPLC≥98.0%
Molecular Formula C10H15N3Na3O14P3
Molecular Weight 563.10
Storage Condition -20℃

5-Me-CTP, 100mM solution

5-methyl-cytidine-5'-triphosphate, sodium salt solution
Grade
Amount/Package: 0.1mL / centrifuge tube
SKU: R3-029-UL100-P
Collections:  Modified Triphosphates mRNA Nucleotides Nucleotides for mRNA Other Raw Materials Synthesis Blocks
Unit price:
$22.50

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Details

5-Methylcytidine-5’-triphosphate (5mCTP) is a chemically modified CTP analog in which a methyl group is introduced at the 5-position of the cytosine base.

Incorporation of m5C into mRNA has been shown to reduce innate immune recognition while improving transcript stability and translational efficiency.

The 100 mM trisodium salt solution provides 5mCTP in a highly soluble, ready-to-use format, suitable for both research-scale experiments and reproducible manufacturing workflows.

Applications

Modified RNA Synthesis

In vitro transcription (IVT) of chemically modified mRNA to modulate immunogenicity and improve expression profiles.[1]

Epitranscriptomic Method Development and Validation

Generation of m5C-containing RNA standards for the development, calibration, and validation of transcriptome-wide m5C detection methods.[2]

Features and Benefits

High purity, nuclease-free, and Ultra-low endotoxin: suitable for sensitive biochemical assays.
Ready-to-use: 100 mM solution eliminates the need for weighing and dissolution.
Stable disodium salt form: ensures consistent pH and long-term storage.

Reference

[1] Son, Sora, Minsa Park, Jin Kim, and Kyuri Lee. "ACE mRNA (additional chimeric element incorporated IVT mRNA) for enhancing protein expression by modulating immunogenicity." Advanced Science 11, no. 18 (2024): 2307541. [2] Edelheit, Sarit, Schraga Schwartz, Maxwell R. Mumbach, Omri Wurtzel, and Rotem Sorek. "Transcriptome-wide mapping of 5-methylcytidine RNA modifications in bacteria, archaea, and yeast reveals m5C within archaeal mRNAs." PLoS genetics 9, no. 6 (2013): e1003602.

Specifications
  • Catalog No.
    R3-029
  • CAS No.
    327174-86-7 (free acid)
  • SMILES
    NC(C(C)=CN1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O)O2)=NC1=O
  • Molecular Formula
    C10H15N3Na3O14P3
  • Molecular Weight
    563.10
  • Appearance
    Clear colorless solution
  • Purity
    HPLC≥98.0%
  • Storage Condition
    -20℃
  • Quality Control
    This lot of NTP has been performance tested in transcription reaction. This preparation is free of DNase and RNase contamination.

Documentation
COA Contact Us

5-Methylcytidine-5’-triphosphate (5mCTP) is a chemically modified CTP analog in which a methyl group is introduced at the 5-position of the cytosine base.

Incorporation of m5C into mRNA has been shown to reduce innate immune recognition while improving transcript stability and translational efficiency.

The 100 mM trisodium salt solution provides 5mCTP in a highly soluble, ready-to-use format, suitable for both research-scale experiments and reproducible manufacturing workflows.

Applications

Modified RNA Synthesis

In vitro transcription (IVT) of chemically modified mRNA to modulate immunogenicity and improve expression profiles.[1]

Epitranscriptomic Method Development and Validation

Generation of m5C-containing RNA standards for the development, calibration, and validation of transcriptome-wide m5C detection methods.[2]

Features and Benefits

High purity, nuclease-free, and Ultra-low endotoxin: suitable for sensitive biochemical assays.
Ready-to-use: 100 mM solution eliminates the need for weighing and dissolution.
Stable disodium salt form: ensures consistent pH and long-term storage.

Reference

[1] Son, Sora, Minsa Park, Jin Kim, and Kyuri Lee. "ACE mRNA (additional chimeric element incorporated IVT mRNA) for enhancing protein expression by modulating immunogenicity." Advanced Science 11, no. 18 (2024): 2307541. [2] Edelheit, Sarit, Schraga Schwartz, Maxwell R. Mumbach, Omri Wurtzel, and Rotem Sorek. "Transcriptome-wide mapping of 5-methylcytidine RNA modifications in bacteria, archaea, and yeast reveals m5C within archaeal mRNAs." PLoS genetics 9, no. 6 (2013): e1003602.

Why choose Hongene?

Trusted Partner in Nucleic Acid

Integrated Supply & Commercial Scale

With 26+ years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

Proprietary Technology & IP

Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

Rigorous Quality

We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

Manufacturing Scalability

Hongene operates 1.67 million sq. ft Oligonucleotide Manufacturing Facility, with advanced equipments including multiple OligoPilot™ and OligoProcess™ synthesizers (10-1800 mmol). 48 flexible production lines enable one-stop seamless scaling-up of API production from gram-level to tons and acheive high purity of 98%, meeting NMPA, FDA, and EMA standards.

Global Business Network

Our products and services reach over 40 countries and regions, supporting around 3,000 clients worldwide.

Global Business Distribution
40+
Countries & Regions
3000≈
Global Clients
54000+L
Annual NTP Production
58+t
Annual Amidites Production

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