An example phosphoramidite structure consists of a 5' - protected DMTrO group, a sugar ring, a phosphoramidite moiety (containing diisopropylamino, etc.), and base - related parts.
What are Phosphoramidites? Phosphoramidites are specialized chemical compounds that serve as the essential building blocks in automated solid-phase oligonucleotide synthesis. These molecules consist of a nucleoside (adenosine, guanosine, cytidine, thymidine, or uridine, often with protective groups and sugar modifications) attached to a reactive phosphoramidite group, typically with a dialkylamine moiety (e.g., diisopropylamine) that acts as the leaving group during coupling reactions.
Synthesis and Key Characteristics Our phosphoramidites are manufactured through a meticulous multi-step process:
Nucleoside Preparation: Starting nucleosides are carefully protected at exocyclic amines (with groups like acetyl, benzoyl, or isobutyryl) and at the 5'-hydroxyl position (typically with DMTr or MMTr groups) to prevent undesired reactions during synthesis.
Phosphitylation: The protected nucleoside reacts with a phosphitylating reagent (e.g., 2-cyanoethyl N,N-diisopropylchlorophosphoramidite) under controlled anhydrous conditions to form the phosphoramidite.
Purification and Stabilization: The crude product undergoes rigorous purification through chromatography and/or crystallization. Stabilizers may be added to enhance shelf life.
Quality Control: Each batch undergoes comprehensive analysis including HPLC for purity assessment, ³¹P NMR to confirm phosphoramidite structure and purity, and moisture analysis to ensure stability.
Application in Oligonucleotide Synthesis Phosphoramidites are designed for use in automated synthesizers following a four-step cycle:
Detritylation: Acidic removal of the 5'-protecting group (DMTr)
Coupling: Activation with weak acid (e.g., tetrazole) enables nucleophilic attack by the free 5'-OH of the growing chain, forming a phosphite triester linkage
Capping: Acetylation of unreacted chains prevents deletion sequences
Oxidation: Conversion of the phosphite triester to a more stable phosphate triester
Our Advantages
High Purity & Structural Integrity
Every batch of none-customization amidites achieves a typical purity up to ≥98%, as verified by advanced analytical techniques including HPLC and ³¹P NMR spectroscopy.
Exceptional Coupling Efficiency
Our phosphoramidites consistently achieve excellent coupling efficiencies, enabling synthesis of long and complex oligonucleotides with high yields and minimal failure sequences.
Comprehensive Portfolio
We offer standard and modified phosphoramidites (including 2'-O-methyl, 2'-fluoro, LNA, and many other modifications) to meet diverse research and therapeutic development needs.
Powering Innovation Across Life Sciences
Our phosphoramidites are trusted by scientists worldwide to synthesize oligonucleotides for a vast range of cutting-edge applications
Therapeutic Development
Synthesize critical components for siRNA, antisense oligonucleotides (ASOs), and aptamers for novel drug discovery and development. Our high-purity phosphoramidites ensure the reliability and efficacy of therapeutic oligonucleotides.
Diagnostics & PCR
Produce highly specific PCR primers, probes, and capture sequences for molecular diagnostics and genetic testing. Achieve exceptional accuracy and reproducibility with our consistent quality.
Research Tools
Enable foundational research in genomics, including gene synthesis, mutagenesis studies, and NGS library preparation. Our products support academic and industrial research with reliable performance.
Next-Generation Sequencing
Manufacture of primers, adapters, and other oligonucleotides used in NGS library preparation and target enrichment.
CRISPR/Cas Systems
Production of guide RNAs (gRNAs) and related components for genome editing applications.
Nanotechnology
Fabrication of DNA origami and other nucleic acid nanostructures for material science and drug delivery applications.
