DMT-dT-CE-Phosphoramidite

5'-O-(4, 4'-Dimethoxytrityl)-thymidine-3'-cyanoethyl Phosphoramidite

Catalog No.	PD4-002
CAS No.	98796-51-1
Purity	HPLC≥98.0%
Molecular Formula	C₄₀H₄₉N₄O₈P
Molecular Weight	744.81
Storage Condition	-20℃

DMT-dT-CE-Phosphoramidite

5'-O-(4, 4'-Dimethoxytrityl)-thymidine-3'-cyanoethyl Phosphoramidite

Grade

N (Normal)

Amount/Package: 0.25g / 30mL screwed bottle-28

0.25g / 30mL screwed bottle-28

0.5g / 30mL screwed bottle-28

1g / 30mL screwed bottle-28

5g / 240mL screwed bottle

10g / 240mL screwed bottle

25g / 250mL HDPE bottle

100g / 250mL HDPE bottle

200g / 500mL HDPE bottle

Product variants

SKU: PD4-002-025A | suitable for MerMade

Collections: Diagnostics DNA Phosphoramidites Oligo Synthesis Phosphoramidites Phosphoramidites for Diagnostics Synthesis Blocks

Price

$4.00

Shipping calculated at checkout.

This variant is unavailable. Contact us if you have particular requirements.

This variant only support customization. Click the Inquiry button below to submit your custom request to us.

Price/Order Inquiry

Shipping notes

Products on this site:

Currently ship to United States & Canada.

For shipping worldwide, please contact us.

Processment: within 2 business days, if available.

US: 2-8 days (from CA by FedEx).

Canada: 5-10 days (excludes customs delays).

Details

DMT-dT-CE-Phosphoramidite is a standard DNA phosphoramidite monomer used for solid-phase oligonucleotide synthesis^[1]^[2]. It contains a 5’-dimethoxytrityl (DMT) protecting group for 5’-hydroxyl protection, a cyanoethyl (CE) protecting group on phosphite, and a thymine.

This phosphoramidite enables the incorporation of deoxythymidine (dT) residues into synthetic DNA-containing oligonucleotides with high coupling efficiency and sequence fidelity.

Applications

Therapeutic Oligonucleotide

As a core component for DNA-based aptamers, antisense oligonucleotides, or hybrid oligonucleotides (e.g., DNA–RNA chimeras).

Hybridization probes

Used to generate DNA probes for microarrays, in situ hybridization, or diagnostic assays.

Molecular biology tools

For assembling standard or modified DNA sequences used in PCR, qPCR, cloning, sequencing primers, CRISPR guide templates, and NGS barcodes.

Features and Benefits

High Coupling Efficiency

High coupling efficiency and reproducibility in solid-phase DNA synthesis

Wide Compatibility

Compatible with standard phosphoramidite chemistry and most commercial DNA synthesizers

Excellent Purity

Produces oligonucleotides with excellent purity and predictable deprotection behavior

Flexible Scale

Suitable for both research-scale and GMP-grade oligonucleotide production

Security Information

Storage: Store in a dry, inert atmosphere at -20 °C.
Recommended deprotection: 8 h at 55 °C in concentrated ammonia, or 10 min at 65 °C in AMA (ammonia/methylamine, 1:1 v/v).
Compatibility: Can be used alongside modified phosphoramidites (e.g., 2′-OMe, 2′-MOE, LNA) to synthesize chimeric oligonucleotides.

Reference

[1] Beaucage, S. L., and Marvin H. Caruthers. "Deoxynucleoside phosphoramidites—a new class of key intermediates for deoxypolynucleotide synthesis." Tetrahedron letters 22, no. 20 (1981): 1859-1862.
[2] Caruthers, M. H., A. D. Barone, S. L. Beaucage, D. R. Dodds, E. F. Fisher, L. J. McBride, M. Matteucci, Z. Stabinsky, and J-Y. Tang. "[15] Chemical synthesis of deoxyoligonucleotides by the phosphoramidite method." In Methods in enzymology, vol. 154, pp. 287-313. Academi c Press, 1987.

Specifications

Catalog No.

PD4-002
PD4-002
PD4-002
PD4-002
PD4-002
PD4-002
PD4-002
PD4-002
CAS No.

98796-51-1
98796-51-1
98796-51-1
98796-51-1
98796-51-1
98796-51-1
98796-51-1
98796-51-1
SMILES

O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
Molecular Formula

C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
Molecular Weight

744.81
744.81
744.81
744.81
744.81
744.81
744.81
744.81
Appearance

White to off-white powder
White to off-white powder
White to off-white powder
White to off-white powder
White to off-white powder
White to off-white powder
White to off-white powder
White to off-white powder
Purity

HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
Storage Condition

-20℃
-20℃
-20℃
-20℃
-20℃
-20℃
-20℃
-20℃
Moisture Content

K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w

Documentation

COA Contact Us

This phosphoramidite enables the incorporation of deoxythymidine (dT) residues into synthetic DNA-containing oligonucleotides with high coupling efficiency and sequence fidelity.

Applications

Therapeutic Oligonucleotide

As a core component for DNA-based aptamers, antisense oligonucleotides, or hybrid oligonucleotides (e.g., DNA–RNA chimeras).

Hybridization probes

Used to generate DNA probes for microarrays, in situ hybridization, or diagnostic assays.

Molecular biology tools

For assembling standard or modified DNA sequences used in PCR, qPCR, cloning, sequencing primers, CRISPR guide templates, and NGS barcodes.

Features and Benefits

High Coupling Efficiency

High coupling efficiency and reproducibility in solid-phase DNA synthesis

Wide Compatibility

Compatible with standard phosphoramidite chemistry and most commercial DNA synthesizers

Excellent Purity

Produces oligonucleotides with excellent purity and predictable deprotection behavior

Flexible Scale

Suitable for both research-scale and GMP-grade oligonucleotide production

Security Information

Storage: Store in a dry, inert atmosphere at -20 °C.
Recommended deprotection: 8 h at 55 °C in concentrated ammonia, or 10 min at 65 °C in AMA (ammonia/methylamine, 1:1 v/v).
Compatibility: Can be used alongside modified phosphoramidites (e.g., 2′-OMe, 2′-MOE, LNA) to synthesize chimeric oligonucleotides.

Reference

Catalog No.

PD4-002
PD4-002
PD4-002
PD4-002
PD4-002
PD4-002
PD4-002
PD4-002
CAS No.

98796-51-1
98796-51-1
98796-51-1
98796-51-1
98796-51-1
98796-51-1
98796-51-1
98796-51-1
SMILES

O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
O=C1C(C)=CN([C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2)C(N1)=O
Molecular Formula

C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
C₄₀H₄₉N₄O₈P
Molecular Weight

744.81
744.81
744.81
744.81
744.81
744.81
744.81
744.81
Appearance

White to off-white powder
White to off-white powder
White to off-white powder
White to off-white powder
White to off-white powder
White to off-white powder
White to off-white powder
White to off-white powder
Purity

HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
HPLC≥98.0%
Storage Condition

-20℃
-20℃
-20℃
-20℃
-20℃
-20℃
-20℃
-20℃
Moisture Content

K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w
K.F.≤0.20% w/w

Why choose Hongene?

Trusted Partner in Nucleic Acid

Integrated Supply & Commercial Scale

With 26+ years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

Proprietary Technology & IP

Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

Rigorous Quality

We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

Manufacturing Scalability

Hongene operates 1.67 million sq. ft Oligonucleotide Manufacturing Facility, with advanced equipments including multiple OligoPilot™ and OligoProcess™ synthesizers (10-1800 mmol). 48 flexible production lines enable one-stop seamless scaling-up of API production from gram-level to tons and acheive high purity of 98%, meeting NMPA, FDA, and EMA standards.

Global Business Network

Our products and services reach over 40 countries and regions, supporting around 3,000 clients worldwide.

Global Business Distribution

40+

Countries & Regions

3000≈

Global Clients

54000+L

Annual NTP Production

58+t

Annual Amidites Production