N1-Me-pUTP, 200mM Tris Salt Solution

N1-methyl-pseudouridine 5'-triphosphate, tris salt solution

Catalog No.	R5-064
Purity	HPLC≥97.0%
Molecular Formula	C₁₀H₁₇N₂O₁₅P₃ (free acid)
Molecular Weight	498.17 (free acid)
Storage Condition	-20℃

N1-Me-pUTP, 200mM Tris Salt Solution

N1-methyl-pseudouridine 5'-triphosphate, tris salt solution

Grade

M (Molecular Biology)+S (Endotoxin)

Amount/Package: 0.1mL / centrifuge tube

0.1mL / centrifuge tube

1mL / centrifuge tube

5mL / nalgene bottle

Product variants

SKU: R5-064-UL100-P

Collections: Modified Triphosphates mRNA Nucleotides Nucleotides for mRNA Other Raw Materials Synthesis Blocks

Price

$65.00

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Shipping notes

Products on this site:

Currently ship to United States & Canada.

For shipping worldwide, please contact us.

Processment: within 2 business days, if available.

US: 2-8 days (from CA by FedEx).

Canada: 5-10 days (excludes customs delays).

Details

N1-methyl-pseudouridine-5’-triphosphate (m¹ΨTP) is a chemically modified uridine nucleotide designed for enzymatic incorporation into RNA during IVT using RNA polymerases such as T7 RNA polymerase.

m¹ΨTP is an N1-methylated derivative of pseudouridine that preserves base-pairing properties while altering innate immune recognition. When substituted for uridine in IVT, m¹Ψ reduces activation of innate immune sensors and enhances translational efficiency of the resulting mRNA.

This product is supplied as a 200 mM aqueous solution in the Tris salt form, providing a stable, mildly alkaline environment (typically pH 7.5-8.5) that enhances compatibility with biochemical and enzymatic assays.

Applications

IVT & RNA Engineering

Used in IVT systems to generate high-quality and modified mRNA with improved performance.^[1][2]

mRNA Therapeutics & Vaccines

Used for the production of therapeutic mRNA, where m¹Ψ provides stronger suppression of innate immune responses and supports robust protein expression in vivo.^[3][4]

Features

High purity, nuclease-free, and extra-low endotoxin: suitable for sensitive biochemical assays.

Ready-to-use: 200 mM solution eliminates the need for weighing and dissolution.

Stable disodium salt form: ensures consistent pH and long-term storage.

Reference

[1] Svitkin, Yuri V., Yi Min Cheng, Tirtha Chakraborty, Vladimir Presnyak, Matthias John, and Nahum Sonenberg. "N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density." Nucleic acids research 45, no. 10 (2017): 6023-6036. [2] Chen, Tien-Hao, Vladimir Potapov, Nan Dai, Jennifer L. Ong, and Bijoyita Roy. "N 1-methyl-pseudouridine is incorporated with higher fidelity than pseudouridine in synthetic RNAs." Scientific Reports 12, no. 1 (2022): 13017. [3] Morais, Pedro, Hironori Adachi, and Yi-Tao Yu. "The critical contribution of pseudouridine to mRNA COVID-19 vaccines." Frontiers in cell and developmental biology 9 (2021): 789427. [4] 4.Rubio-Casillas, Alberto, David Cowley, Mikolaj Raszek, Vladimir N. Uversky, and Elrashdy M. Redwan. "N1-methyl-pseudouridine (m1Ψ): Friend or foe of cancer?." International Journal of Biological Macromolecules 267 (2024): 131427.

Specifications

Catalog No.

R5-064
R5-064
R5-064
SMILES

OCC(N)(CO)CO.O=C(N(C=C1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(OP(OP([O-])([O-])=O)([O-])=O)([O-])=O)O2)C)NC1=O
OCC(N)(CO)CO.O=C(N(C=C1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(OP(OP([O-])([O-])=O)([O-])=O)([O-])=O)O2)C)NC1=O
OCC(N)(CO)CO.O=C(N(C=C1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(OP(OP([O-])([O-])=O)([O-])=O)([O-])=O)O2)C)NC1=O
Molecular Formula

C₁₀H₁₇N₂O₁₅P₃ (free acid)
C₁₀H₁₇N₂O₁₅P₃ (free acid)
C₁₀H₁₇N₂O₁₅P₃ (free acid)
Molecular Weight

498.17 (free acid)
498.17 (free acid)
498.17 (free acid)
Appearance

Clear colorless to yellow solution
Clear colorless to yellow solution
Clear colorless to yellow solution
Purity

HPLC≥97.0%
HPLC≥97.0%
HPLC≥97.0%
Storage Condition

-20℃
-20℃
-20℃
Bacterial Indotocxin Test

<1 EU/ml
<1 EU/ml
<1 EU/ml
Quality Control

This lot of NTP has been performance tested in transcription reaction. This preparation is free of DNase and RNase contamination.
This lot of NTP has been performance tested in transcription reaction. This preparation is free of DNase and RNase contamination.
This lot of NTP has been performance tested in transcription reaction. This preparation is free of DNase and RNase contamination.

Documentation

COA Contact Us

Applications

IVT & RNA Engineering

Used in IVT systems to generate high-quality and modified mRNA with improved performance.^[1][2]

mRNA Therapeutics & Vaccines

Used for the production of therapeutic mRNA, where m¹Ψ provides stronger suppression of innate immune responses and supports robust protein expression in vivo.^[3][4]

Features

High purity, nuclease-free, and extra-low endotoxin: suitable for sensitive biochemical assays.

Ready-to-use: 200 mM solution eliminates the need for weighing and dissolution.

Stable disodium salt form: ensures consistent pH and long-term storage.

Reference

Catalog No.

R5-064
R5-064
R5-064
SMILES

OCC(N)(CO)CO.O=C(N(C=C1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(OP(OP([O-])([O-])=O)([O-])=O)([O-])=O)O2)C)NC1=O
OCC(N)(CO)CO.O=C(N(C=C1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(OP(OP([O-])([O-])=O)([O-])=O)([O-])=O)O2)C)NC1=O
OCC(N)(CO)CO.O=C(N(C=C1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(OP(OP([O-])([O-])=O)([O-])=O)([O-])=O)O2)C)NC1=O
Molecular Formula

C₁₀H₁₇N₂O₁₅P₃ (free acid)
C₁₀H₁₇N₂O₁₅P₃ (free acid)
C₁₀H₁₇N₂O₁₅P₃ (free acid)
Molecular Weight

498.17 (free acid)
498.17 (free acid)
498.17 (free acid)
Appearance

Clear colorless to yellow solution
Clear colorless to yellow solution
Clear colorless to yellow solution
Purity

HPLC≥97.0%
HPLC≥97.0%
HPLC≥97.0%
Storage Condition

-20℃
-20℃
-20℃
Bacterial Indotocxin Test

<1 EU/ml
<1 EU/ml
<1 EU/ml
Quality Control

This lot of NTP has been performance tested in transcription reaction. This preparation is free of DNase and RNase contamination.
This lot of NTP has been performance tested in transcription reaction. This preparation is free of DNase and RNase contamination.
This lot of NTP has been performance tested in transcription reaction. This preparation is free of DNase and RNase contamination.

Why choose Hongene?

Trusted Partner in Nucleic Acid

Integrated Supply & Commercial Scale

With 26+ years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

Proprietary Technology & IP

Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

Rigorous Quality

We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

Manufacturing Scalability

Hongene operates 1.67 million sq. ft Oligonucleotide Manufacturing Facility, with advanced equipments including multiple OligoPilot™ and OligoProcess™ synthesizers (10-1800 mmol). 48 flexible production lines enable one-stop seamless scaling-up of API production from gram-level to tons and acheive high purity of 98%, meeting NMPA, FDA, and EMA standards.

Global Business Network

Our products and services reach over 40 countries and regions, supporting around 3,000 clients worldwide.

Global Business Distribution

40+

Countries & Regions

3000≈

Global Clients

54000+L

Annual NTP Production

58+t

Annual Amidites Production