DNA
1μg
BspQI
| Catalog No. | ON-124 |
| Storage Condition | -20℃ |
| Source | Recombinant E.coli |
| Purity | ＞95% by SDS-PAGE |
BspQI
Catalog No: ON-124
BspQI - M (Molecular Biology) / Non-GMP / 10kU / centrifuge tube is backordered and will ship as soon as it is back in stock.
SKU:
ON-124-KU010-P
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Shipping notes
Shipping notes
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Delivery Time:- US: 3-8 business days (FedEx Standard).
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Excerpt from Shipping Policy
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Details
Details
Product Information
Product description: BspQI is a thermostable Type IIS restriction endonuclease (REase) with the recognition sequence 5'-GCTCTTCN1/N4-3'
Source: Recombinant E.coli
Concentration: 10U/μL
Unit Definition: One unit is defined as the amount of enzyme required to digest 1μg of λDNA in 1X Cut Buffer following 1 hour incubation at 50°C in a total reaction volume of 50μL.
Storage Buffer: 20mM Tris-HCl, 0.1mM EDTA, 500mM KCl, 1mM DTT, 0.1%Triton X-100, 50%(v/v) glycerol, pH7.0, 25℃.
Companion Product: 10X Cut Buffer with BSA, Cat#ON-187, 500mM Tris-HCl, 1000mM NaCl, 100mM MgCl2, 1mg/mL BSA, pH7.9, 25℃.
Key points of operation
Restriction Digest Protocol:
- On ice, combine the following reaction components in the order given:
Component
Amount
10X Cut Buffer
5μL
BspQI
10 units
Total Reaction Volume
50μL
- Incubate at 50℃, 60min.
- Heat inactivity enzyme by incubating at 80℃, 2min.
Methylation Sensitivity: Not CpG methylation-sensitive, not dam methylation-sensitive, not dcm methylation-sensitive.
Enzyme stability: BspQI half-life period at 50℃ is 2 hours in 1X Cut Buffer.
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
Specifications
Specifications
-
Catalog No.ON-124ON-124-G
-
SourceRecombinant E.coliRecombinant E.coli
-
Purity＞95% by SDS-PAGE＞95% by SDS-PAGE
-
Storage Condition-20℃-20℃
Documentation
Documentation
Product Information
Product description: BspQI is a thermostable Type IIS restriction endonuclease (REase) with the recognition sequence 5'-GCTCTTCN1/N4-3'
Source: Recombinant E.coli
Concentration: 10U/μL
Unit Definition: One unit is defined as the amount of enzyme required to digest 1μg of λDNA in 1X Cut Buffer following 1 hour incubation at 50°C in a total reaction volume of 50μL.
Storage Buffer: 20mM Tris-HCl, 0.1mM EDTA, 500mM KCl, 1mM DTT, 0.1%Triton X-100, 50%(v/v) glycerol, pH7.0, 25℃.
Companion Product: 10X Cut Buffer with BSA, Cat#ON-187, 500mM Tris-HCl, 1000mM NaCl, 100mM MgCl2, 1mg/mL BSA, pH7.9, 25℃.
Key points of operation
Restriction Digest Protocol:
- On ice, combine the following reaction components in the order given:
Component
Amount
DNA
1μg
10X Cut Buffer
5μL
BspQI
10 units
Total Reaction Volume
50μL
- Incubate at 50℃, 60min.
- Heat inactivity enzyme by incubating at 80℃, 2min.
Methylation Sensitivity: Not CpG methylation-sensitive, not dam methylation-sensitive, not dcm methylation-sensitive.
Enzyme stability: BspQI half-life period at 50℃ is 2 hours in 1X Cut Buffer.
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
-
Catalog No.ON-124ON-124-G
-
SourceRecombinant E.coliRecombinant E.coli
-
Purity＞95% by SDS-PAGE＞95% by SDS-PAGE
-
Storage Condition-20℃-20℃
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