DMT-2'-F-dG(dmf)-CE Phosphoramidite

2'-fluoro-N2-dimethylformamidine-5'-O-(4, 4'-dimethoxytrityl)-2'-deoxyguanosine-3'-CEPhosphoramidite
Catalog No. PD2-001
Purity HPLC ≥98.0%
Molecular Formula C43H52FN8O7P
Molecular Weight 842.89
Storage Condition -20℃

DMT-2'-F-dG(dmf)-CE Phosphoramidite

2'-fluoro-N2-dimethylformamidine-5'-O-(4, 4'-dimethoxytrityl)-2'-deoxyguanosine-3'-CEPhosphoramidite
Grade
Amount/Package: 0.25g / 30mL screwed bottle-28
SKU: PD2-001-025A  |  suitable for  MerMade
Collections:  2'-Fluoro Phosphoramidites Oligo Synthesis Phosphoramidites Synthesis Blocks
Unit price:
$63.00

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    Details

    DMT-2’-F-dG(dmf)-CE-Phosphoramidite is a 2'-fluoro (2'-F) phosphoramidite monomer used for solid-phase oligonucleotide synthesis.

    It contains a 2'-F modification on the sugar of deoxyguanosine (dG) with dimethylformamidyl (dmf) base protection, a 5’-dimethoxytrityl (DMT) group for 5'-hydroxyl protection, and a cyanoethyl (CE) protecting group on phosphite.

    Synthesis of RNA oligonucleotides using 2'-F phosphoramidite increases the nuclease resistance and binding affinity of duplexes formed with complementary sequences.

    Applications

    Therapeutic oligonucleotides

    Commonly used in chemically modified small interfering RNA (siRNA) to enhance stability, potency, and in-vivo performance.[1][2]

    Probing and diagnostics

    For constructing nuclease-resistant and high-affinity probes (e.g, aptamers) to study biomolecule interactions,[3][4] which can be related to diseases and cancers.[5][6]

    Features and Benefits

    High purity: Manufactured to ≥98% purity with tightly controlled impurity specifications.
    Compatibility: Compatible with standard phosphoramidite chemistry and most commercial synthesizers.
    Excellent coupling efficiency and yield.

    Other Notes

    • Diluent: Anhydrous Acetonitrile.
    • Storage: Store in a dry and inert atmosphere at -20 °C.
    • Coupling: 6 minute coupling time recommended.

    Reference

    [1] Manoharan, M., Akinc, A., Pandey, R.K., Qin, J., Hadwiger, P., John, M., Mills, K., Charisse, K., Maier, M.A., Nechev, L. and Greene, E.M., 2011. Unique gene‐silencing and structural properties of 2′‐fluoro‐modified siRNAs. Angewandte Chemie, 123(10), pp.2332-2336. [2] Dowler, Thomas, Denis Bergeron, Anna-Lisa Tedeschi, Luc Paquet, Nicolay Ferrari, and Masad J. Damha. "Improvements in siRNA properties mediated by 2′-deoxy-2′-fluoro-β-D-arabinonucleic acid (FANA)." Nucleic acids research 34, no. 6 (2006): 1669-1675. [3] Kratschmer, Christina, and Matthew Levy. "Effect of chemical modifications on aptamer stability in serum." Nucleic acid therapeutics 27, no. 6 (2017): 335-344. [4] Hervas-Stubbs, Sandra, Mario M. Soldevilla, Helena Villanueva, Uxua Mancheño, Maurizio Bendandi, and Fernando Pastor. "Identification of TIM3 2′-fluoro oligonucleotide aptamer by HT-SELEX for cancer immunotherapy." Oncotarget 7, no. 4 (2015): 4522. [5] Gruenke, Paige R., Khalid K. Alam, Kamal Singh, and Donald H. Burke. "2′-fluoro-modified pyrimidines enhance affinity of RNA oligonucleotides to HIV-1 reverse transcriptase." RNA 26, no. 11 (2020): 1667-1679. [6] Lozano, Teresa, Mario Martínez Soldevilla, Noelia Casares, Helena Villanueva, Maurizio Bendandi, Juan Jose Lasarte, and Fernando Pastor. "Targeting inhibition of Foxp3 by a CD28 2′-Fluro oligonucleotide aptamer conjugated to P60-peptide enhances active cancer immunotherapy." Biomaterials 91 (2016): 73-80.

    Specifications
    • Catalog No.
      PD2-001
    • SMILES
      F[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C2=CC=C(OC)C=C2)(C3=CC=CC=C3)C4=CC=C(OC)C=C4)O[C@H]1N(C=N5)C6=C5C(NC(/N=C/N(C)C)=N6)=O
    • Molecular Formula
      C43H52FN8O7P
    • Molecular Weight
      842.89
    • Appearance
      White to off white powder, free from visible foreign matter
    • Purity
      HPLC ≥98.0%
    • Storage Condition
      -20℃
    • Moisture Content
      K.F.≤0.30% w/w

    Documentation
    COA Contact Us

    DMT-2’-F-dG(dmf)-CE-Phosphoramidite is a 2'-fluoro (2'-F) phosphoramidite monomer used for solid-phase oligonucleotide synthesis.

    It contains a 2'-F modification on the sugar of deoxyguanosine (dG) with dimethylformamidyl (dmf) base protection, a 5’-dimethoxytrityl (DMT) group for 5'-hydroxyl protection, and a cyanoethyl (CE) protecting group on phosphite.

    Synthesis of RNA oligonucleotides using 2'-F phosphoramidite increases the nuclease resistance and binding affinity of duplexes formed with complementary sequences.

    Applications

    Therapeutic oligonucleotides

    Commonly used in chemically modified small interfering RNA (siRNA) to enhance stability, potency, and in-vivo performance.[1][2]

    Probing and diagnostics

    For constructing nuclease-resistant and high-affinity probes (e.g, aptamers) to study biomolecule interactions,[3][4] which can be related to diseases and cancers.[5][6]

    Features and Benefits

    High purity: Manufactured to ≥98% purity with tightly controlled impurity specifications.
    Compatibility: Compatible with standard phosphoramidite chemistry and most commercial synthesizers.
    Excellent coupling efficiency and yield.

    Other Notes

    • Diluent: Anhydrous Acetonitrile.
    • Storage: Store in a dry and inert atmosphere at -20 °C.
    • Coupling: 6 minute coupling time recommended.

    Reference

    [1] Manoharan, M., Akinc, A., Pandey, R.K., Qin, J., Hadwiger, P., John, M., Mills, K., Charisse, K., Maier, M.A., Nechev, L. and Greene, E.M., 2011. Unique gene‐silencing and structural properties of 2′‐fluoro‐modified siRNAs. Angewandte Chemie, 123(10), pp.2332-2336. [2] Dowler, Thomas, Denis Bergeron, Anna-Lisa Tedeschi, Luc Paquet, Nicolay Ferrari, and Masad J. Damha. "Improvements in siRNA properties mediated by 2′-deoxy-2′-fluoro-β-D-arabinonucleic acid (FANA)." Nucleic acids research 34, no. 6 (2006): 1669-1675. [3] Kratschmer, Christina, and Matthew Levy. "Effect of chemical modifications on aptamer stability in serum." Nucleic acid therapeutics 27, no. 6 (2017): 335-344. [4] Hervas-Stubbs, Sandra, Mario M. Soldevilla, Helena Villanueva, Uxua Mancheño, Maurizio Bendandi, and Fernando Pastor. "Identification of TIM3 2′-fluoro oligonucleotide aptamer by HT-SELEX for cancer immunotherapy." Oncotarget 7, no. 4 (2015): 4522. [5] Gruenke, Paige R., Khalid K. Alam, Kamal Singh, and Donald H. Burke. "2′-fluoro-modified pyrimidines enhance affinity of RNA oligonucleotides to HIV-1 reverse transcriptase." RNA 26, no. 11 (2020): 1667-1679. [6] Lozano, Teresa, Mario Martínez Soldevilla, Noelia Casares, Helena Villanueva, Maurizio Bendandi, Juan Jose Lasarte, and Fernando Pastor. "Targeting inhibition of Foxp3 by a CD28 2′-Fluro oligonucleotide aptamer conjugated to P60-peptide enhances active cancer immunotherapy." Biomaterials 91 (2016): 73-80.

    Why choose Hongene?

    Trusted Partner in Nucleic Acid

    Enjoy enterprise‑grade quality even with small batch orders.

    Integrated Supply & Commercial Scale

    With nearly 30 years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

    Proprietary Technology & IP

    Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

    Rigorous Quality

    We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

    Manufacturing Scalability

    Hongene operates a 1.67-million-square-foot oligonucleotide manufacturing facility featuring advanced equipment, including multiple OligoPilot™ and OligoProcess™ synthesizers ranging from 10 to 1,800 mmol. Its 48 flexible production lines enable seamless, one-stop scale-up of API production from gram-level quantities to metric tons, achieving purity of ≥98% while supporting compliance with NMPA, FDA, and EMA regulatory requirements.

    Global Business Network

    Our products and services reach over 40 countries and regions, supporting around 3,000 clients worldwide.

    Global Business Distribution
    40+
    Countries & Regions
    ≈3000
    Global Clients
    54000L+
    Annual NTP Production
    58t+
    Annual Amidites Production

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