Pyrophosphatase Inorganic (yeast)
| Catalog No. | ON-025 |
| Purity | ＞95% by SDS-PAGE |
| Storage Condition | -20℃ |
| Source | Recombinant E.coli |
Pyrophosphatase Inorganic (yeast)
Pyrophosphatase Inorganic (yeast) - M (Molecular Biology) / Non-GMP / 0.1kU / centrifuge tube is backordered and will ship as soon as it is back in stock.
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Details
Details
Product Information
PPase is prepared from an E. coli strain containing Saccharomyces cerevisiae ppa gene. It is a homodimer consisting of two equal subunits of molecular weight 32-35kDa. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates.
Source: Recombinant E.coli
Concentration: 0.1U/μL
Unit Definition: One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25℃ in 100mM Tris-HCl, [pH 7.2], 2mM MgCl2 and 2mM PPi in a reaction volume of 0.5mL)
Storage Buffer: 20mM Tris-HCl PH8.0, 1mM DTT, 0.1mM EDTA, 50% Glycerol.
Key points of operation
When in vitro transcription, higher yields of RNA may be obtained by adding inorganic pyrophosphatase to a final concentration of 4units/mL.
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
Reference
1. Cooperman, B.S., The mechanism of action of yeast inorganic pyrophosphatase, Meth. Enzymol., 87, 526-548, 1982.
2. Cunningham, P.R. and Ofengand, J., Use of inorganic pyrophosphatase to improve the yield of in vitro transcription reactions catalyzed by T7 RNA polymerase, Biotechniques, 9, 713-714, 1990.
Specifications
Specifications
-
Catalog No.ON-025ON-025-G
-
SourceRecombinant E.coliRecombinant E.coli
-
Purity＞95% by SDS-PAGE＞95% by SDS-PAGE
-
Storage Condition-20℃-20℃
Documentation
Documentation
Product Information
PPase is prepared from an E. coli strain containing Saccharomyces cerevisiae ppa gene. It is a homodimer consisting of two equal subunits of molecular weight 32-35kDa. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates.
Source: Recombinant E.coli
Concentration: 0.1U/μL
Unit Definition: One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25℃ in 100mM Tris-HCl, [pH 7.2], 2mM MgCl2 and 2mM PPi in a reaction volume of 0.5mL)
Storage Buffer: 20mM Tris-HCl PH8.0, 1mM DTT, 0.1mM EDTA, 50% Glycerol.
Key points of operation
When in vitro transcription, higher yields of RNA may be obtained by adding inorganic pyrophosphatase to a final concentration of 4units/mL.
Security Information
Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
Reference
1. Cooperman, B.S., The mechanism of action of yeast inorganic pyrophosphatase, Meth. Enzymol., 87, 526-548, 1982.
2. Cunningham, P.R. and Ofengand, J., Use of inorganic pyrophosphatase to improve the yield of in vitro transcription reactions catalyzed by T7 RNA polymerase, Biotechniques, 9, 713-714, 1990.
-
Catalog No.ON-025ON-025-G
-
SourceRecombinant E.coliRecombinant E.coli
-
Purity＞95% by SDS-PAGE＞95% by SDS-PAGE
-
Storage Condition-20℃-20℃
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