Pyrophosphatase Inorganic (yeast)

Catalog No. ON-025
Storage Condition -20℃
Source Recombinant E.coli
Purity ＞95% by SDS-PAGE

Pyrophosphatase Inorganic (yeast)

Grade
GMP level
Amount/Package: 0.1kU / centrifuge tube
SKU: ON-025-U100-P
Unit price:
$120.00

Price

$120.00
Shipping calculated at checkout.
This variant is unavailable. Contact us if you have particular requirements.
Checkout

This variant only support customization. Click the Inquiry button below to submit your custom request to us.

Price/Order Inquiry

Shipping notes

Products in shop.hongene.com currently can only ship to the United States and Canada. For other regions, please visit contact us page to leave us a message or contact our regional sales.

Processing Time: within 2 business days, if available.

Delivery Time:
  • US: 3-8 business days (FedEx Standard).
  • Canada: 5-10 business days (excludes customs delays).

Note: Delays due to force majeure like customs, natural disasters, or strikes are beyond our liability.

Excerpt from Shipping Policy

Details

Product Information

Product description: PPase is prepared from an E. coli strain containing Saccharomyces cerevisiae ppa gene. It is a homodimer consisting of two equal subunits of molecular weight 32-35kDa. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates.

Source: Recombinant E.coli

Concentration: 0.1U/μL

Unit Definition: One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25℃ in 100mM Tris-HCl, [pH 7.2], 2mM MgCl2 and 2mM PPi in a reaction volume of 0.5mL)

Storage Buffer: 20mM Tris-HCl PH8.0, 1mM DTT, 0.1mM EDTA, 50% Glycerol.

Key points of operation

When in vitro transcription, higher yields of RNA may be obtained by adding inorganic pyrophosphatase to a final concentration of 4units/mL.

Security Information

Storage Conditions: -20℃

Quality Assurance: Free of endonuclease, exonuclease and RNase activities.

Physical Purity: ＞95% by SDS-PAGE.

Reference

1. Cooperman, B.S., The mechanism of action of yeast inorganic pyrophosphatase, Meth. Enzymol., 87, 526-548, 1982.

2. Cunningham, P.R. and Ofengand, J., Use of inorganic pyrophosphatase to improve the yield of in vitro transcription reactions catalyzed by T7 RNA polymerase, Biotechniques, 9, 713-714, 1990.

Specifications
  • Catalog No.
    ON-025
  • Source
    Recombinant E.coli
  • Purity
    ＞95% by SDS-PAGE
  • Storage Condition
    -20℃

Documentation
COA Contact Us

Product Information

Product description: PPase is prepared from an E. coli strain containing Saccharomyces cerevisiae ppa gene. It is a homodimer consisting of two equal subunits of molecular weight 32-35kDa. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates.

Source: Recombinant E.coli

Concentration: 0.1U/μL

Unit Definition: One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25℃ in 100mM Tris-HCl, [pH 7.2], 2mM MgCl2 and 2mM PPi in a reaction volume of 0.5mL)

Storage Buffer: 20mM Tris-HCl PH8.0, 1mM DTT, 0.1mM EDTA, 50% Glycerol.

Key points of operation

When in vitro transcription, higher yields of RNA may be obtained by adding inorganic pyrophosphatase to a final concentration of 4units/mL.

Security Information

Storage Conditions: -20℃

Quality Assurance: Free of endonuclease, exonuclease and RNase activities.

Physical Purity: ＞95% by SDS-PAGE.

Reference

1. Cooperman, B.S., The mechanism of action of yeast inorganic pyrophosphatase, Meth. Enzymol., 87, 526-548, 1982.

2. Cunningham, P.R. and Ofengand, J., Use of inorganic pyrophosphatase to improve the yield of in vitro transcription reactions catalyzed by T7 RNA polymerase, Biotechniques, 9, 713-714, 1990.

Related Products

Back to top

My Wishlist