Pyrophosphatase Inorganic (yeast)

Product Information

PPase is prepared from an E. coli strain containing Saccharomyces cerevisiae ppa gene. It is a homodimer consisting of two equal subunits of molecular weight 32-35kDa. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates.

Source: Recombinant E.coli
Concentration: 0.1U/μL
Unit Definition: One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25℃ in 100mM Tris-HCl, [pH 7.2], 2mM MgCl₂ and 2mM PPi in a reaction volume of 0.5mL)
Storage Buffer: 20mM Tris-HCl PH8.0, 1mM DTT, 0.1mM EDTA, 50% Glycerol.

Key points of operation

When in vitro transcription, higher yields of RNA may be obtained by adding inorganic pyrophosphatase to a final concentration of 4units/mL.

Security Information

Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.

Reference

1. Cooperman, B.S., The mechanism of action of yeast inorganic pyrophosphatase, Meth. Enzymol., 87, 526-548, 1982.
2. Cunningham, P.R. and Ofengand, J., Use of inorganic pyrophosphatase to improve the yield of in vitro transcription reactions catalyzed by T7 RNA polymerase, Biotechniques, 9, 713-714, 1990.