DMT-2'-O-TBDMS-G(iBu)-CE-Phosphoramidite

N2-isobutyryl-5'-O-(4, 4'-dimethoxytrityl)-2'-O-(t-butyl-dimethylsilyl)-guanosine-3'-cyanoethyl Phosphoramidite
Catalog No. PR2-007
CAS No. 147201-04-5
Purity HPLC≥98.0%
Molecular Formula C50H68N7O9PSi
Molecular Weight 970.18
Storage Condition -20℃

DMT-2'-O-TBDMS-G(iBu)-CE-Phosphoramidite

N2-isobutyryl-5'-O-(4, 4'-dimethoxytrityl)-2'-O-(t-butyl-dimethylsilyl)-guanosine-3'-cyanoethyl Phosphoramidite
Grade
Amount/Package: 0.25g / 30mL screwed bottle-28
SKU: PR2-007-025A  |  suitable for  MerMade
Collections:  Diagnostics Oligo Synthesis Phosphoramidites Phosphoramidites for Diagnostics RNA (2'-O-TBDMS) Phosphoramidites Synthesis Blocks
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$20.00

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Details

DMT-2'-O-TBDMS-G(iBu)-CE-Phosphoramidite is a protected ribonucleoside building block used for solid-phase RNA oligonucleotide synthesis. It contains a 5'-dimethoxytrityl (DMT) protecting group for 5'-hydroxyl protection, a 2'-O-tert-butyldimethylsilyl (TBDMS) protecting group for 2'-hydroxyl protection, a cyanoethyl (CE) protecting group on phosphite, and a isobutyryl (iBu) base-protecting group on guanine.

TBDMS offers excellent stability for protecting 2'-hydroxyl of ribonucleosides during acid detritylation and oxidation steps.

TBDMS deprotection can be achieved using fluoride reagents (e.g., tetrabutylammonium fluoride (TBAF) and triethylamine trihydrofluoride (TEA·3HF)), which can cleave the silyl group without damaging the RNA backbone.

Applications

Synthetic RNA oligonucleotides

Monomer for preparing functional RNA sequences used in biochemical assays and binding studies,[1] such as RNA aptamers and ribozymes.

Mixed backbone design

Enables the synthesis of RNA-DNA chimeras, e.g., chimeric RNA/DNA oligonucleotide-based gene therapy.[2][3]

Features and Benefits

Stable during acidic detritylation and oxidative steps
Compatibility with other ribonucleoside phosphoramidites
High coupling efficiency under standard RNA synthesis conditions
Flexible deprotection schemes, i.e., compatible with TEA·3HF, or other fluoride sources for TBDMS removal

Other Notes

  • Storage: Store in a dry, inert atmosphere at -20 °C.
  • Coupling: 12 minutes coupling time recommended
  • Compatibility: Can be used alongside modified phosphoramidites (e.g., 2′-OMe, 2′-MOE, Locked-NA) to synthesize chimeric oligonucleotides.

Reference

[1] Sproat, Brian S. "RNA synthesis using 2′-O-(tert-butyldimethylsilyl) protection." In Oligonucleotide Synthesis, pp. 17-31. Totowa, NJ: Humana Press, 2005. [2] Lai, Li-Wen, and Yeong-Hau H. Lien. "Chimeric RNA/DNA oligonucleotide-based gene therapy." Kidney International 61, no. 1 (2002): S47-S51. [3] Li, Nan-Sheng, John K. Frederiksen, and Joseph A. Piccirilli. "Synthesis, properties, and applications of oligonucleotides containing an RNA dinucleotide phosphorothiolate linkage." Accounts of chemical research 44, no. 12 (2011): 1257-1269.

Specifications
  • Catalog No.
    PR2-007
  • CAS No.
    147201-04-5
  • SMILES
    C[Si](C)(C(C)(C)C)O[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C2=CC=C(OC)C=C2)(C3=CC=CC=C3)C4=CC=C(OC)C=C4)O[C@H]1N(C=N5)C6=C5C(NC(NC(C(C)C)=O)=N6)=O
  • Molecular Formula
    C50H68N7O9PSi
  • Molecular Weight
    970.18
  • Appearance
    White to faint yellow powder
  • Purity
    HPLC≥98.0%
  • Storage Condition
    -20℃
  • Moisture Content
    K.F.≤0.20% w/w

Documentation
COA Contact Us

DMT-2'-O-TBDMS-G(iBu)-CE-Phosphoramidite is a protected ribonucleoside building block used for solid-phase RNA oligonucleotide synthesis. It contains a 5'-dimethoxytrityl (DMT) protecting group for 5'-hydroxyl protection, a 2'-O-tert-butyldimethylsilyl (TBDMS) protecting group for 2'-hydroxyl protection, a cyanoethyl (CE) protecting group on phosphite, and a isobutyryl (iBu) base-protecting group on guanine.

TBDMS offers excellent stability for protecting 2'-hydroxyl of ribonucleosides during acid detritylation and oxidation steps.

TBDMS deprotection can be achieved using fluoride reagents (e.g., tetrabutylammonium fluoride (TBAF) and triethylamine trihydrofluoride (TEA·3HF)), which can cleave the silyl group without damaging the RNA backbone.

Applications

Synthetic RNA oligonucleotides

Monomer for preparing functional RNA sequences used in biochemical assays and binding studies,[1] such as RNA aptamers and ribozymes.

Mixed backbone design

Enables the synthesis of RNA-DNA chimeras, e.g., chimeric RNA/DNA oligonucleotide-based gene therapy.[2][3]

Features and Benefits

Stable during acidic detritylation and oxidative steps
Compatibility with other ribonucleoside phosphoramidites
High coupling efficiency under standard RNA synthesis conditions
Flexible deprotection schemes, i.e., compatible with TEA·3HF, or other fluoride sources for TBDMS removal

Other Notes

  • Storage: Store in a dry, inert atmosphere at -20 °C.
  • Coupling: 12 minutes coupling time recommended
  • Compatibility: Can be used alongside modified phosphoramidites (e.g., 2′-OMe, 2′-MOE, Locked-NA) to synthesize chimeric oligonucleotides.

Reference

[1] Sproat, Brian S. "RNA synthesis using 2′-O-(tert-butyldimethylsilyl) protection." In Oligonucleotide Synthesis, pp. 17-31. Totowa, NJ: Humana Press, 2005. [2] Lai, Li-Wen, and Yeong-Hau H. Lien. "Chimeric RNA/DNA oligonucleotide-based gene therapy." Kidney International 61, no. 1 (2002): S47-S51. [3] Li, Nan-Sheng, John K. Frederiksen, and Joseph A. Piccirilli. "Synthesis, properties, and applications of oligonucleotides containing an RNA dinucleotide phosphorothiolate linkage." Accounts of chemical research 44, no. 12 (2011): 1257-1269.

Why choose Hongene?

Trusted Partner in Nucleic Acid

Integrated Supply & Commercial Scale

With 26+ years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

Proprietary Technology & IP

Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

Rigorous Quality

We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

Manufacturing Scalability

Hongene operates 1.67 million sq. ft Oligonucleotide Manufacturing Facility, with advanced equipments including multiple OligoPilot™ and OligoProcess™ synthesizers (10-1800 mmol). 48 flexible production lines enable one-stop seamless scaling-up of API production from gram-level to tons and acheive high purity of 98%, meeting NMPA, FDA, and EMA standards.

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Our products and services reach over 40 countries and regions, supporting around 3,000 clients worldwide.

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