DMT-2'-O-TBDMS-G(iBu)-CE Phosphoramidite

N2-isobutyryl-5'-O-(4, 4'-dimethoxytrityl)-2'-O-(t-butyl-dimethylsilyl)-guanosine-3'-cyanoethyl Phosphoramidite
Catalog No. PR2-007
CAS No. 147201-04-5
Purity HPLC ≥98.0%
Molecular Formula C50H68N7O9PSi
Molecular Weight 970.18
Storage Condition -20℃

DMT-2'-O-TBDMS-G(iBu)-CE Phosphoramidite

N2-isobutyryl-5'-O-(4, 4'-dimethoxytrityl)-2'-O-(t-butyl-dimethylsilyl)-guanosine-3'-cyanoethyl Phosphoramidite
Grade
Amount/Package: 0.25g / 30mL screwed bottle-28
SKU: PR2-007-025A  |  suitable for  MerMade
Collections:  Diagnostics Oligo Synthesis Phosphoramidites Phosphoramidites for Diagnostics RNA (2'-O-TBDMS) Phosphoramidites Synthesis Blocks
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    Details

    DMT-2'-O-TBDMS-G(iBu)-CE-Phosphoramidite is a protected ribonucleoside building block used for solid-phase RNA oligonucleotide synthesis. It contains a 5'-dimethoxytrityl (DMT) protecting group for 5'-hydroxyl protection, a 2'-O-tert-butyldimethylsilyl (TBDMS) protecting group for 2'-hydroxyl protection, a cyanoethyl (CE) protecting group on phosphite, and a isobutyryl (iBu) base-protecting group on guanine.

    TBDMS offers excellent stability for protecting 2'-hydroxyl of ribonucleosides during acid detritylation and oxidation steps.

    TBDMS deprotection can be achieved using fluoride reagents (e.g., tetrabutylammonium fluoride (TBAF) and triethylamine trihydrofluoride (TEA·3HF)), which can cleave the silyl group without damaging the RNA backbone.

    Applications

    Synthetic RNA oligonucleotides

    Monomer for preparing functional RNA sequences used in biochemical assays and binding studies,[1] such as RNA aptamers and ribozymes.

    Mixed backbone design

    Enables the synthesis of RNA-DNA chimeras, e.g., chimeric RNA/DNA oligonucleotide-based gene therapy.[2][3]

    Features and Benefits

    Stable during acidic detritylation and oxidative steps
    Compatibility with other ribonucleoside phosphoramidites
    High coupling efficiency under standard RNA synthesis conditions
    Flexible deprotection schemes, i.e., compatible with TEA·3HF, or other fluoride sources for TBDMS removal

    Other Notes

    • Storage: Store in a dry, inert atmosphere at -20 °C.
    • Coupling: 12 minutes coupling time recommended
    • Compatibility: Can be used alongside modified phosphoramidites (e.g., 2′-OMe, 2′-MOE, Locked-NA) to synthesize chimeric oligonucleotides.

    Reference

    [1] Sproat, Brian S. "RNA synthesis using 2′-O-(tert-butyldimethylsilyl) protection." In Oligonucleotide Synthesis, pp. 17-31. Totowa, NJ: Humana Press, 2005. [2] Lai, Li-Wen, and Yeong-Hau H. Lien. "Chimeric RNA/DNA oligonucleotide-based gene therapy." Kidney International 61, no. 1 (2002): S47-S51. [3] Li, Nan-Sheng, John K. Frederiksen, and Joseph A. Piccirilli. "Synthesis, properties, and applications of oligonucleotides containing an RNA dinucleotide phosphorothiolate linkage." Accounts of chemical research 44, no. 12 (2011): 1257-1269.

    Specifications
    • Catalog No.
      PR2-007
    • CAS No.
      147201-04-5
    • SMILES
      C[Si](C)(C(C)(C)C)O[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C2=CC=C(OC)C=C2)(C3=CC=CC=C3)C4=CC=C(OC)C=C4)O[C@H]1N(C=N5)C6=C5C(NC(NC(C(C)C)=O)=N6)=O
    • Molecular Formula
      C50H68N7O9PSi
    • Molecular Weight
      970.18
    • Appearance
      White to light yellow powder, free from visible foreign matter
    • Purity
      HPLC ≥98.0%
    • Storage Condition
      -20℃
    • Moisture Content
      K.F.≤0.20% w/w

    Documentation
    COA Contact Us

    DMT-2'-O-TBDMS-G(iBu)-CE-Phosphoramidite is a protected ribonucleoside building block used for solid-phase RNA oligonucleotide synthesis. It contains a 5'-dimethoxytrityl (DMT) protecting group for 5'-hydroxyl protection, a 2'-O-tert-butyldimethylsilyl (TBDMS) protecting group for 2'-hydroxyl protection, a cyanoethyl (CE) protecting group on phosphite, and a isobutyryl (iBu) base-protecting group on guanine.

    TBDMS offers excellent stability for protecting 2'-hydroxyl of ribonucleosides during acid detritylation and oxidation steps.

    TBDMS deprotection can be achieved using fluoride reagents (e.g., tetrabutylammonium fluoride (TBAF) and triethylamine trihydrofluoride (TEA·3HF)), which can cleave the silyl group without damaging the RNA backbone.

    Applications

    Synthetic RNA oligonucleotides

    Monomer for preparing functional RNA sequences used in biochemical assays and binding studies,[1] such as RNA aptamers and ribozymes.

    Mixed backbone design

    Enables the synthesis of RNA-DNA chimeras, e.g., chimeric RNA/DNA oligonucleotide-based gene therapy.[2][3]

    Features and Benefits

    Stable during acidic detritylation and oxidative steps
    Compatibility with other ribonucleoside phosphoramidites
    High coupling efficiency under standard RNA synthesis conditions
    Flexible deprotection schemes, i.e., compatible with TEA·3HF, or other fluoride sources for TBDMS removal

    Other Notes

    • Storage: Store in a dry, inert atmosphere at -20 °C.
    • Coupling: 12 minutes coupling time recommended
    • Compatibility: Can be used alongside modified phosphoramidites (e.g., 2′-OMe, 2′-MOE, Locked-NA) to synthesize chimeric oligonucleotides.

    Reference

    [1] Sproat, Brian S. "RNA synthesis using 2′-O-(tert-butyldimethylsilyl) protection." In Oligonucleotide Synthesis, pp. 17-31. Totowa, NJ: Humana Press, 2005. [2] Lai, Li-Wen, and Yeong-Hau H. Lien. "Chimeric RNA/DNA oligonucleotide-based gene therapy." Kidney International 61, no. 1 (2002): S47-S51. [3] Li, Nan-Sheng, John K. Frederiksen, and Joseph A. Piccirilli. "Synthesis, properties, and applications of oligonucleotides containing an RNA dinucleotide phosphorothiolate linkage." Accounts of chemical research 44, no. 12 (2011): 1257-1269.

    Why choose Hongene?

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    Integrated Supply & Commercial Scale

    With nearly 30 years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

    Proprietary Technology & IP

    Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

    Rigorous Quality

    We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

    Manufacturing Scalability

    Hongene operates a 1.67-million-square-foot oligonucleotide manufacturing facility featuring advanced equipment, including multiple OligoPilot™ and OligoProcess™ synthesizers ranging from 10 to 1,800 mmol. Its 48 flexible production lines enable seamless, one-stop scale-up of API production from gram-level quantities to metric tons, achieving purity of ≥98% while supporting compliance with NMPA, FDA, and EMA regulatory requirements.

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