General structure of a seccinate
What are Succinates?
Succinates, in the context of oligonucleotide synthesis, refer to succinyl-based linker molecules that are covalently attached to solid supports—such as controlled pore glass (CPG), polystyrene, or other polymeric matrices—to serve as the initial anchoring point for synthesis. These molecules feature a terminal carboxyl group that is activated to form a stable ester or amide bond with the first nucleotide of the growing oligonucleotide chain, enabling efficient and reliable solid-phase synthesis.
Synthesis and Functionalization
Our succinate-functionalized supports are manufactured through a controlled and reproducible process:
- Support Activation: The solid support (e.g., CPG) is first chemically modified with amino or hydroxyl functional groups to provide reactive sites.
- Succinylation: Succinic anhydride or its derivatives are reacted with the functionalized support under optimized conditions to form a succinyl monolayer with terminal carboxylic acid groups.
- Activation and Functionalization: The carboxyl groups are activated (e.g., using NHS or carbodiimide chemistry) to allow covalent coupling to the 3'-end of the first nucleoside (or appropriate modifier), resulting in a stable, synthesis-ready support.
- Rigorous QC: Each batch is validated for loading capacity, homogeneity, and stability to ensure consistent performance.
Role in Oligonucleotide Synthesis
Succinate-based linkers are essential in solid-phase oligonucleotide synthesis, where they:
- Serve as a stable, hydrolytically robust anchor between the solid support and the oligonucleotide.
- Allow for efficient chain elongation under standard phosphoramidite chemistry conditions.
- Enable clean and complete cleavage of the synthesized oligonucleotide under specific conditions (e.g., ammonium hydroxide treatment).
