5-Me-DMT-dC(Ac)-CE Phosphoramidite

N4-acetyl-5-methyl-5'-O-(4, 4'-dimethoxytrityl)-2'-deoxycytidine-3'-cyanoethyl Phosphoramidite
Catalog No. PD3-009
Purity HPLC ≥98.0%
Molecular Formula C42H52N5O8P
Molecular Weight 785.86
Storage Condition -20℃

5-Me-DMT-dC(Ac)-CE Phosphoramidite

N4-acetyl-5-methyl-5'-O-(4, 4'-dimethoxytrityl)-2'-deoxycytidine-3'-cyanoethyl Phosphoramidite
Grade
Amount/Package: 0.25g / 30mL screwed bottle-28
SKU: PD3-009-025A  |  suitable for  MerMade
Collections:  DNA Phosphoramidites Oligo Synthesis Phosphoramidites Synthesis Blocks
Unit price:
$49.00
Price
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    Details

    5-Me-DMT-dC(Ac)-CE-Phosphoramidite is a standard DNA phosphoramidite monomer used for solid-phase oligonucleotide synthesis[1][2]. It contains a 5’-dimethoxytrityl (DMT) protecting group for 5’-hydroxyl protection, a cyanoethyl (CE) protecting group on phosphite, and a acetyl (Ac) base-protecting group on 5-methylcytosine.

    This phosphoramidite enables the incorporation of deoxy-5-methylcytidine (5-Me-dC) residues into synthetic DNA-containing oligonucleotides with high coupling efficiency and sequence fidelity.

    Applications

    Therapeutic Oligonucleotide

    As a core component for DNA-based aptamers, antisense oligonucleotides, or hybrid oligonucleotides (e.g., DNA–RNA chimeras).

    Hybridization probes

    Used to generate DNA probes for microarrays, in situ hybridization, or diagnostic assays.

    Molecular biology tools

    For assembling standard or modified DNA sequences used in PCR, qPCR, cloning, sequencing primers, CRISPR guide templates, and NGS barcodes.

    Features and Benefits

    1

    High Coupling Efficiency

    High coupling efficiency and reproducibility in solid-phase DNA synthesis

    2

    Wide Compatibility

    Compatible with standard phosphoramidite chemistry and most commercial DNA synthesizers

    3

    Excellent Purity

    Produces oligonucleotides with excellent purity and predictable deprotection behavior

    4

    Flexible Scale

    Suitable for both research-scale and GMP-grade oligonucleotide production

    Security Information

    • Storage: Store in a dry, inert atmosphere at -20 °C.
    • Recommended deprotection: 8 h at 55 °C in concentrated ammonia, or 10 min at 65 °C in AMA (ammonia/methylamine, 1:1 v/v).
    • Compatibility: Can be used alongside modified phosphoramidites (e.g., 2′-OMe, 2′-MOE, LNA) to synthesize chimeric oligonucleotides.

    Reference

    [1] Beaucage, S. L., and Marvin H. Caruthers. "Deoxynucleoside phosphoramidites—a new class of key intermediates for deoxypolynucleotide synthesis." Tetrahedron letters 22, no. 20 (1981): 1859-1862.
    [2] Caruthers, M. H., A. D. Barone, S. L. Beaucage, D. R. Dodds, E. F. Fisher, L. J. McBride, M. Matteucci, Z. Stabinsky, and J-Y. Tang. "[15] Chemical synthesis of deoxyoligonucleotides by the phosphoramidite method." In Methods in enzymology, vol. 154, pp. 287-313. Academi c Press, 1987.

    Specifications
    • Catalog No.
      PD3-009
    • SMILES
      O=C1N=C(NC(C)=O)C(C)=CN1[C@H]2C[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(C3=CC=C(OC)C=C3)(C4=CC=CC=C4)C5=CC=C(OC)C=C5)O2
    • Molecular Formula
      C42H52N5O8P
    • Molecular Weight
      785.86
    • Appearance
      White, off-white to faint yellow powder, free from visible foreign matter
    • Purity
      HPLC ≥98.0%
    • Storage Condition
      -20℃
    • Moisture Content
      K.F.≤0.20% w/w

    Documentation
    COA Contact Us

    5-Me-DMT-dC(Ac)-CE-Phosphoramidite is a standard DNA phosphoramidite monomer used for solid-phase oligonucleotide synthesis[1][2]. It contains a 5’-dimethoxytrityl (DMT) protecting group for 5’-hydroxyl protection, a cyanoethyl (CE) protecting group on phosphite, and a acetyl (Ac) base-protecting group on 5-methylcytosine.

    This phosphoramidite enables the incorporation of deoxy-5-methylcytidine (5-Me-dC) residues into synthetic DNA-containing oligonucleotides with high coupling efficiency and sequence fidelity.

    Applications

    Therapeutic Oligonucleotide

    As a core component for DNA-based aptamers, antisense oligonucleotides, or hybrid oligonucleotides (e.g., DNA–RNA chimeras).

    Hybridization probes

    Used to generate DNA probes for microarrays, in situ hybridization, or diagnostic assays.

    Molecular biology tools

    For assembling standard or modified DNA sequences used in PCR, qPCR, cloning, sequencing primers, CRISPR guide templates, and NGS barcodes.

    Features and Benefits

    1

    High Coupling Efficiency

    High coupling efficiency and reproducibility in solid-phase DNA synthesis

    2

    Wide Compatibility

    Compatible with standard phosphoramidite chemistry and most commercial DNA synthesizers

    3

    Excellent Purity

    Produces oligonucleotides with excellent purity and predictable deprotection behavior

    4

    Flexible Scale

    Suitable for both research-scale and GMP-grade oligonucleotide production

    Security Information

    • Storage: Store in a dry, inert atmosphere at -20 °C.
    • Recommended deprotection: 8 h at 55 °C in concentrated ammonia, or 10 min at 65 °C in AMA (ammonia/methylamine, 1:1 v/v).
    • Compatibility: Can be used alongside modified phosphoramidites (e.g., 2′-OMe, 2′-MOE, LNA) to synthesize chimeric oligonucleotides.

    Reference

    [1] Beaucage, S. L., and Marvin H. Caruthers. "Deoxynucleoside phosphoramidites—a new class of key intermediates for deoxypolynucleotide synthesis." Tetrahedron letters 22, no. 20 (1981): 1859-1862.
    [2] Caruthers, M. H., A. D. Barone, S. L. Beaucage, D. R. Dodds, E. F. Fisher, L. J. McBride, M. Matteucci, Z. Stabinsky, and J-Y. Tang. "[15] Chemical synthesis of deoxyoligonucleotides by the phosphoramidite method." In Methods in enzymology, vol. 154, pp. 287-313. Academi c Press, 1987.

    Why choose Hongene?

    Trusted Partner in Nucleic Acid

    Enjoy enterprise‑grade quality even with small batch orders.

    Integrated Supply & Commercial Scale

    With nearly 30 years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.

    Proprietary Technology & IP

    Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.

    Rigorous Quality

    We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.

    Manufacturing Scalability

    Hongene operates a 1.67-million-square-foot oligonucleotide manufacturing facility featuring advanced equipment, including multiple OligoPilot™ and OligoProcess™ synthesizers ranging from 10 to 1,800 mmol. Its 48 flexible production lines enable seamless, one-stop scale-up of API production from gram-level quantities to metric tons, achieving purity of ≥98% while supporting compliance with NMPA, FDA, and EMA regulatory requirements.

    Global Business Network

    Our products and services reach over 40 countries and regions, supporting around 3,000 clients worldwide.

    Global Business Distribution
    40+
    Countries & Regions
    ≈3000
    Global Clients
    54000L+
    Annual NTP Production
    58t+
    Annual Amidites Production

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