T3 DNA ligase
| Catalog No. | ON-1188 |
| Purity | ＞95% by SDS-PAGE |
| Storage Condition | -20℃ |
| Source | Recombinant E.coli |
T3 DNA ligase
T3 DNA ligase - M (Molecular Biology) / Non-GMP is backordered and will ship as soon as it is back in stock.
Couldn't load pickup availability
Shipping notes
Shipping notes
Details
Details
Product Information
T3 DNA Ligase is an ATP-dependent dsDNA ligase derived from bacteriophage T3 and recombinantly expressed in E. coli. It catalyzes the formation of phosphodiester bonds between adjacent 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA (dsDNA), with higher efficiency for A/T overhangs than C/G overhangs. The enzyme efficiently ligates dsDNA with either sticky or blunt ends, repairs nicks in dsDNA, and seals nicks in DNA/RNA hybrids containing an intact DNA strand. Consequently, T3 DNA Ligase can be used to generate DNA-RNA chimeras and to ligate RNA fragments using a DNA template.
Similar to T4 DNA Ligase, the addition of PEG 6000 significantly enhances blunt-end ligation efficiency. T3 DNA Ligase exhibits twice the salt tolerance of T4 DNA Ligase, retaining >95% activity in 1.0 M NaCl or KCl, making it an ideal choice for ligation under high-salt conditions.
Specifications
Specifications
-
Catalog No.ON-1188
-
SourceRecombinant E.coli
-
Purity＞95% by SDS-PAGE
-
Storage Condition-20℃
Documentation
Documentation
Product Information
T3 DNA Ligase is an ATP-dependent dsDNA ligase derived from bacteriophage T3 and recombinantly expressed in E. coli. It catalyzes the formation of phosphodiester bonds between adjacent 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA (dsDNA), with higher efficiency for A/T overhangs than C/G overhangs. The enzyme efficiently ligates dsDNA with either sticky or blunt ends, repairs nicks in dsDNA, and seals nicks in DNA/RNA hybrids containing an intact DNA strand. Consequently, T3 DNA Ligase can be used to generate DNA-RNA chimeras and to ligate RNA fragments using a DNA template.
Similar to T4 DNA Ligase, the addition of PEG 6000 significantly enhances blunt-end ligation efficiency. T3 DNA Ligase exhibits twice the salt tolerance of T4 DNA Ligase, retaining >95% activity in 1.0 M NaCl or KCl, making it an ideal choice for ligation under high-salt conditions.
-
Catalog No.ON-1188
-
SourceRecombinant E.coli
-
Purity＞95% by SDS-PAGE
-
Storage Condition-20℃
Why choose Hongene?
Trusted Partner in Nucleic Acid
Enjoy enterprise‑grade quality even with small batch orders.
Integrated Supply & Commercial Scale
With nearly 30 years of expertise, we control a secure supply chain for RNA raw materials and provide reliable GMP-grade oligo synthesis from research to commercial kilogram-scale production.
Proprietary Technology & IP
Our proprietary Chemoenzymatic Ligation Platform combines chemical andenzymatic methods, enabling high-putity, cost-effective, and large-scale production of RNA-based therapeutics.
Rigorous Quality
We implement multiple stringent QC steps, maintain ISO certifications, and ensure >99% batch-to-batch consistency, reducing scale-up and PPQ risks.
Manufacturing Scalability
Hongene operates a 1.67-million-square-foot oligonucleotide manufacturing facility featuring advanced equipment, including multiple OligoPilot™ and OligoProcess™ synthesizers ranging from 10 to 1,800 mmol. Its 48 flexible production lines enable seamless, one-stop scale-up of API production from gram-level quantities to metric tons, achieving purity of ≥98% while supporting compliance with NMPA, FDA, and EMA regulatory requirements.
Global Business Network
Our products and services reach over 40 countries and regions, supporting around 3,000 clients worldwide.
