BspQI

Product Information

Product description: BspQI is a thermostable Type IIS restriction endonuclease (REase) with the recognition sequence 5'-GCTCTTCN1/N4-3'

Source: Recombinant E.coli

Concentration: 10U/μL

Unit Definition: One unit is defined as the amount of enzyme required to digest 1μg of λDNA in 1X Cut Buffer following 1 hour incubation at 50°C in a total reaction volume of 50μL.

Storage Buffer: 20mM Tris-HCl, 0.1mM EDTA, 500mM KCl, 1mM DTT, 0.1%Triton X-100, 50%(v/v) glycerol, pH7.0, 25℃.

Companion Product: 10X Cut Buffer with BSA, Cat#ON-187, 500mM Tris-HCl, 1000mM NaCl, 100mM MgCl₂, 1mg/mL BSA, pH7.9, 25℃.

Key points of operation

Restriction Digest Protocol:

1. On ice, combine the following reaction components in the order given:

2. Incubate at 50℃, 60min.
3. Heat inactivity enzyme by incubating at 80℃, 2min.

Methylation Sensitivity: Not CpG methylation-sensitive, not dam methylation-sensitive, not dcm methylation-sensitive.

Enzyme stability: BspQI half-life period at 50℃ is 2 hours in 1X Cut Buffer.

Security Information

Storage Conditions: -20℃

Quality Assurance: Free of endonuclease, exonuclease and RNase activities.

Physical Purity: ＞95% by SDS-PAGE.